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Iris germanica Jinwawa tissue culture method

A tissue culture and doll technology, which is applied in the field of plant tissue cultivation, can solve the problems such as no report on the tissue culture and plant regeneration technology of golden doll iris, and achieves the effects of fast reproduction, broad market prospects and robust growth.

Inactive Publication Date: 2012-04-04
武汉市林业果树科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, reports on tissue culture and propagation of Iris are limited to several varieties such as Lewis, Sinus, Hybrid Iris, Changchunhuang, etc. There is no report on the tissue culture and plant regeneration technology of Golden Baby Iris.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) After half a month of sunny weather, collect young flower buds with a total length of about 1cm, peel off the outer bracts, keep the innermost layer of bracts, and rinse with running water for 30 minutes for later use.

[0028] 2) The alcohol concentration is 70%, the soaking time is 2s, rinse with sterile water 4 times after pouring out the alcohol, add 0.08% mercuric acid solution, soak for 6 minutes, shake continuously during the period, pour out the mercuric acid solution and rinse with sterile water 5 times.

[0029] 3) After the flower buds are sterilized, blot the surface moisture with sterile filter paper, carefully peel off the innermost bracts with sterile tweezers, cut off the flower buds and ovary with a sterile blade, and cut off the flowers with a thickness of 4mm from the inner bracts in the lower part of the ovary The stalk part was inserted into the sterile induction differentiation medium; the formulation of the induction differentiation medium was ...

Embodiment 2

[0033] 1) After half a month of continuous sunny weather, collect young flower buds with a total length of about 2cm, peel off the outer bracts, keep the innermost layer of bracts, and rinse with running water for 25 minutes for later use.

[0034] 2) The alcohol concentration is 75%, the soaking time is 3s, rinse with sterile water 5 times after pouring out the alcohol, add 0.1% mercuric acid solution, soak for 5 minutes, shake continuously during this period, pour out the mercuric acid solution and rinse with sterile water 6 times.

[0035]3) After the flower buds are sterilized, blot the surface moisture with sterile filter paper, carefully peel off the innermost bracts with sterile tweezers, cut off the flower buds and ovary with a sterile blade, and cut off the flowers with a thickness of 5 mm in the inner bract inserting part of the lower part of the ovary The stalk part was inserted into the sterile induction differentiation medium; the formulation of the induction diff...

Embodiment 3

[0039] 1) After half a month of continuous sunny weather, collect young flower buds with a total length of about 3cm, peel off the outer bracts, keep the innermost layer of bracts, and rinse with running water for 20 minutes for later use.

[0040] 2) The alcohol concentration is 80%, the soaking time is 2s, rinse with sterile water 6 times after pouring out the alcohol, add 0.12% mercuric chloride solution, soak for 4 minutes, shake continuously during this period, pour out the mercuric chloride solution and rinse with sterile water 7 times.

[0041] 3) After the flower buds are sterilized, blot the surface moisture with sterile filter paper, carefully peel off the innermost bracts with sterile tweezers, cut off the flower buds and ovary with a sterile blade, and cut off the 6mm flower of the inner bract inserting part of the lower part of the ovary The stalk part is inserted into the sterile induction differentiation medium; the formula of the induction differentiation mediu...

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PUM

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Abstract

The invention relates to an iris germanica Jinwawa tissue culture method. The iris germanica Jinwawa tissue culture method comprises the following steps of 1, selecting a raw material of an iris germanica Jinwawa seedling growing on the land, cutting off young buds with stalks when the young buds are formed between early-March to mid-April, removing outer bracts of the young buds, and flushing by clear water for next use, 2, carrying out immersion disinfection of the cleaned young buds on a superclean workbench orderly by an alcohol solution and a mercury bichloride solution, and flushing by sterile water, 3, removing inner bracts located at lower parts of the young buds, removing top parts and ovaries of the young buds, and introducing the stalks located at insertion parts of the inner bracts into a medium, 4, culturing for a period of time, and when adventitious buds are formed on the insertion parts of the inner bracts, transferring the adventitious buds to a multiplication medium for culture, and 5, culturing for a period of time, cutting off cluster buds formed from the adventitious buds, and culturing the cut cluster buds in a rooting medium to form strong tissue cultured rooting seedlings. The iris germanica Jinwawa tissue culture method has the advantages of simpleness of disinfection, high survival rate and fast breeding speed.

Description

technical field [0001] The invention belongs to the technical field of plant tissue cultivation, in particular to a tissue cultivation method of German iris golden doll. Background technique [0002] Golden Baby Iris is a species of Iris in the family Iridaceae, the genus German Iris ( Iris germanica A cultivar under L.), German iris is native to Europe and is commonly cultivated in various parts of my country. The golden baby iris was originally a perennial flower introduced from Germany by the Beijing Botanical Garden. The plant height is 30-40 cm. The massive rhizomes are horizontal or inclined, and the leaves are upright, overlapping each other, and light green. The plant diameter is 30-40 cm. The flower stems are pulled out from the leaves, 10-15 flower cymbals per plant, 15-25 cm high, 1-2 flowers per flower cymbal, several bracts at the base of the flower and inflorescence, and 9 in flower diameter cm; petals golden yellow, beard base white, upper part orange. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 董艳芳陈法志郭彩霞童俊戢小梅徐洪亮许林周媛
Owner 武汉市林业果树科学研究所
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