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Blueberry tissue culture method

A tissue culture and blueberry technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as unclear hormone ratio, difficult cultivation success, unreasonable disinfection of single stem segments, etc.

Inactive Publication Date: 2016-12-07
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of blueberry tissue culture method, has solved the hormone ratio in the culture medium that is used for blueberry tissue culture is not clear, is used for the single stem section disinfection of tissue culture unreasonable, and then causes the blueberry tissue survival rate to be low, Cultivate difficult-to-success problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take the blueberry tender shoots that were born in the current year, and cut the 3cm bud stem section;

[0035] (2) Disinfect the above-mentioned stem section with buds with a concentration of 70 volume % alcohol for 10 seconds, then disinfect it with mercuric chloride of 0.08 volume % for 6 minutes, rinse and set aside;

[0036] (3) above-mentioned band bud stem segment is cut into the single stem segment with bud of 1cm long;

[0037] (4) Insert the lower end of the above-mentioned single stem segment into the primary medium (the primary medium is MS medium) for primary culture; wherein, the primary medium also contains zeatin and indolebutyric acid, and relative to 1L The first-generation culture medium, the content of the zeatin is 0.8mg, the content of the indole butyric acid is 0.38mg, sucrose and agar are also added in the first-generation culture medium, relative to the first-generation culture medium of 1L, the content of the sucrose Content is 28g, and the ...

Embodiment 2

[0043] (1) Take the blueberry tender shoots that were born in the current year, and cut the 4cm bud stem section;

[0044] (2) Disinfect the above-mentioned stem segment with buds with a concentration of 75 volume % alcohol for 30 seconds, then disinfect it with mercuric chloride of 0.12 volume % for 8 minutes, rinse and set aside;

[0045] (3) the above-mentioned band bud stem section is cut into the long single stem section with bud of 2cm;

[0046] (4) Insert the lower end of the above-mentioned single stem segment into the primary medium (the primary medium is MS medium) for primary culture; wherein, the primary medium also contains zeatin and indolebutyric acid, and relative to 1L The first-generation culture medium, the content of the zeatin is 1.2mg, the content of the indolebutyric acid is 0.42mg, sucrose and agar are also added in the first-generation culture medium, relative to the first-generation culture medium of 1L, the content of the sucrose Content is 32g, and...

Embodiment 3

[0052] (1) Take the blueberry tender shoots that were born in the current year, and cut the 4cm bud stem section;

[0053] (2) Disinfect the above-mentioned stem section with buds with a concentration of 72 volume % alcohol for 20 seconds, then use a concentration of 0.1 volume % mercury sterilized for 7 minutes, rinse and set aside;

[0054] (3) the above-mentioned band bud stem section is cut into the long single stem section with bud of 2cm;

[0055] (4) Insert the lower end of the above-mentioned single stem segment into the primary medium (the primary medium is MS medium) for primary culture; wherein, the primary medium also contains zeatin and indolebutyric acid, and relative to 1L The first-generation culture medium, the content of the zeatin is 1 mg, the content of the indole butyric acid is 0.4 mg, sucrose and agar are also added in the first-generation culture medium, relative to the first-generation culture medium of 1L, the content of the sucrose 30g, the content ...

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Abstract

The invention discloses a blueberry tissue culture method. The blueberry tissue culture method comprises the steps that annual blueberry shoots are taken, 3-4 cm stem segments with buds are cut, are sterilized with ethyl alcohol with the concentration of 70-75% by volume for 10-30 seconds, then are sterilized with mercury bichloride with the concentration of 0.08-0.12% by volume for 6-8 minutes and are flushed for standby application; the stem segments with buds are cut into single stem segments with buds and with the length of 1-2 cm; the lower ends of the single stem segments are inserted into a primary culture medium for primary culture, and then multiplication culture and rooting culture are performed; by clearly and reasonably setting the hormone contents in the primary culture medium, a multiplication culture medium and a rooting culture medium and conducting reasonable sterilization processing before single stem segment inoculation, blueberry tissue culture is high in survival rate and is easy to succeed.

Description

technical field [0001] The present invention relates to tissue culture, in particular to a blueberry tissue culture method. Background technique [0002] Blueberry (Vaccinium spp.) is a shrub fruit tree of the Rhododendronaceae Vaccinium genus. Blueberry fruit is listed by the United Nations Health Organization as the fruit with the best nutritional value. Therefore, the social demand for blueberries is increasing day by day, and countries have introduced and cultivated them one after another. my country's blueberry industry has developed rapidly since 2009 and is currently mainly distributed in five major regions: Changbai Mountains and Greater and Lesser Xing'an Mountains, Liaodong Peninsula, Jiaodong Peninsula, Yangtze River Basin, Yunnan-Guizhou-Sichuan and South China. With the continuous increase of cultivated area and yield, relevant applied research and applied basic research have attracted more and more attention. Tissue culture has been carried out more on other...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 肖家欣李晴晴樊基胜张春龙鲁珊珊王艳
Owner ANHUI NORMAL UNIV
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