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Rapid propagation method of Pinellia ternata stems

A fast, explanted technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low reproductive efficiency of Pinellia chinensis, and achieve the effects of improved reproductive efficiency, low cost, and obvious production advantages

Active Publication Date: 2016-08-17
湖北道地药材科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, it can be seen that the reproductive efficiency of pinellia is low in the natural state.

Method used

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  • Rapid propagation method of Pinellia ternata stems
  • Rapid propagation method of Pinellia ternata stems
  • Rapid propagation method of Pinellia ternata stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for rapidly propagating the stems of Pinellia chinensis, the steps of which are:

[0026] A﹑Selection and induction of explants: When using petioles as explants, the petioles should be used as explants when the leaves have not yet unfolded. Significantly increased. The induction process of the explants is as follows: remove the petiole explants selected according to the above requirements, rinse them with tap water for 10-30 minutes, then move the above-mentioned petiole explants to a sterilized beaker in the ultra-clean workbench, add 75% (V / V, volume ratio) alcohol, soak for 28 or 29 or 30 or 31 or 32S, pour off the alcohol, wash 2 or 3 or 4 times with sterile water, then add 0.1% (M / V, volume mass Than) bromogeramine soaked for 2 or 3 or 4min, poured off the bromethamine, washed 4 or 5 or 6 times with sterile water, then added 0.2% (M / V, volume to mass ratio) mercuric chloride solution and 1-2 drops of Tween, Soak for 8 or 9 or 10 or 11 or 12 minutes, duri...

Embodiment 2

[0031] A method for rapidly propagating the stems of Pinellia chinensis, the steps of which are:

[0032] A. Selection and induction of explants:

[0033] a, the selection of explants, in order to determine suitable explants, select pinellia tuber, bulbil, petiole and leaf of different development stages as explants, test its pollution rate and induction efficiency. The explant disinfection method is as follows: first rinse with tap water for 15 minutes. In the ultra-clean workbench, transfer the rinsed petiole and leaves to a sterilized bottle, add 75% alcohol to soak for 30 seconds, rinse with sterile water for 3 times, then add 0.01% bromogeramine solution to soak for 5 minutes, and use sterile Rinse with water for 5 times, then soak in 0.1% mercury liter for 10 minutes, and rinse with sterile water for 5 times. The test medium is: MS+TDZ0.5mg / L+NAA0.01mg / LpH5.8. The petiole is cut to a length of about 1 cm, the tuber is cut into a size of about 0.5×0.5×0.5 cm, the bulbi...

Embodiment 3

[0046] A method for rapidly propagating the stems of Pinellia chinensis, the steps of which are:

[0047] A. Proliferation of spheroid buds: In order to proliferate spheroid buds, and at the same time, make the buds disperse and be easy to separate, a series of culture media with different types and concentrations of mitogens are designed. The basic composition of the culture medium is MS, and the amount of sucrose added is 30g / L , the pH value is 5.8. Cut off the bud points on the above-mentioned induced spheroids, about 2-3 bud points as a unit, and inoculate them on the proliferation medium. Inoculate 3 spheroids with 2-3 buds per bottle of medium and inoculate 5 bottles of each medium.

[0048] B, culture condition is identical with embodiment 1 and embodiment 2. After culturing for 25 days, observe the effect of bud growth and dispersion, as shown in Table 3. It can be seen that with the increase of the concentration of 6-BA and TDZ, the bud proliferation efficiency is...

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Abstract

The invention discloses a rapid propagation method of Pinellia ternata stems. The method comprises the steps of: A, selection and induction of explants: selecting expanded petiole as explants, flushing by tap water, adding alcohol, soaking, draining alcohol, washing with sterile water, soaked with bromogeramine, draining the bromogeramine, washing with sterile water, adding mercury bichloride and Tween, soaking, washing with sterile water, inoculating to an induction medium, and culturing to form spheroids with buds; B, proliferation of spheroid buds: inducing to obtain spheroids with buds, cutting the buds in an ultra-clean bench, wherein the cut spheroids contain buds, and conducting proliferation culture; and C, the formation of seed stem: after proliferation culture, taking off single buds, inoculating into a seed stem medium in the ultra-clean bench, and prompting seed stem increase. The method is feasible and easy to operate, can effectively reduce the effects of endophyte on Pinellia ternata sterile culture, improve the efficiency of tissue culture and rapid propagation, and reach high growth efficiency.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and more specifically relates to a method for producing jingpinia seed stems by using plant tissue culture technology. This method is mainly used for the rapid production of Jingpinia seed stems. Background technique [0002] Pinellia ternata (Pinellia ternata) is a perennial herbaceous plant of the Araceae Pinellia genus, with a plant height of 15-30 cm; tubers are nearly spherical, with a diameter of 1-2 cm; leaves come from the top of the tubers, and the petioles are 6-23 cm long. The annual leaves are single Leaves, egg-shaped and heart-shaped; after 2-3 years, the leaves are three compound leaves. Pinellia is a commonly used traditional Chinese medicinal material. Among the 588 kinds of traditional Chinese medicine prescriptions, the frequency of Pinellia ranks 22nd. It has the effects of lowering adverse flow and relieving vomiting, drying damp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李先良李居宁
Owner 湖北道地药材科技有限公司
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