Tissue-culture rapid propagation method for succulents

A technology for rapid propagation of succulents and tissue culture, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long breeding cycle, large site requirements, and high labor costs, and achieve short cultivation time and easy operation , a wide range of effects

Inactive Publication Date: 2016-06-08
四川大巴山生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Small potted succulents are favored by consumers because they are easy to cultivate, have various overall shapes, colors and leaf shapes, and have high ornamental value. Some succ...

Method used

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  • Tissue-culture rapid propagation method for succulents
  • Tissue-culture rapid propagation method for succulents

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Experimental program
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Embodiment 1

[0029] A method for tissue culture and rapid propagation of succulents described in this embodiment comprises the following steps:

[0030] (1) Selection and sterilization of explants: cut young leaves or stems of succulent plants, soak them in detergent solution for 10-30 minutes, then scrub the axillary buds with a brush, rinse them under running water overnight; On a sterile operating table, treat with 75% alcohol for 5-15 seconds, rinse with sterile water for 3-4 times, then treat with 0.1% mercuric chloride for 8-12 minutes, rinse with sterile water for 5-7 times, and dry That's it. Wherein, the time of mercuric chloride treatment is determined according to the degree of tenderness of the explant;

[0031] (2) Primary culture: Inoculate the explants obtained in step (1) on the primary culture medium for primary culture until the explants differentiate and sprout. The composition of the primary culture medium is: MS basic medium supplemented with 0.05 mg / LNAA, 4.0mg / L6-B...

Embodiment 2

[0036] The tissue culture rapid propagation method of a kind of succulent plant described in the present embodiment, differs from embodiment 1 in that:

[0037] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 0.01mg / LNAA, 2.0mg / L6-BA, 0.1g / L hydrolyzed casein, 10g / L sucrose, and 2g / L agar;

[0038] In step (3), the composition of the proliferation medium is: MS basic medium, supplemented with 0.5mg / LKT, 0.1mg / L2, 4-D, 1.0mg / LGA3, 10g / L sucrose, 2g / L agar;

[0039] In step (4), the composition of the rooting medium is: 1 / 4MS basic medium supplemented with 0.08mg / L NAA, 50g / L sucrose, 10g / L agar, and 0.5mg / L activated carbon.

[0040] In the present embodiment, when the multiplication culture process was carried out to 15 days, new shoots germinated at the growth point, and the length of the new shoots reached 5 cm in 30 days, and the new shoots were strong; the rooting culture process began to take root when the rooting culture process ...

Embodiment 3

[0042] The tissue culture rapid propagation method of a kind of succulent plant described in the present embodiment, differs from embodiment 1 in that:

[0043] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 0.08mg / LNAA, 5.0mg / L6-BA, 0.8g / L hydrolyzed casein, 50g / L sucrose, and 10g / L agar;

[0044] In step (3), the composition of the proliferation medium is: MS basic medium, supplemented with 2.0mg / LKT, 0.4mg / L2, 4-D, 5.0mg / LGA3, 50g / L sucrose, 10g / L agar;

[0045] In step (4), the composition of the rooting medium is: 1 / 4MS basic medium supplemented with 0.01mg / L NAA, 10g / L sucrose, 2g / L agar, and 0.1mg / L activated carbon.

[0046] In the present embodiment, when the multiplication culture process was carried out to 15 days, new shoots germinated at the growth point, and the length of the new shoots reached 5 cm in 28 days, and the new shoots were strong; the rooting culture process began to take root when the rooting culture process...

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Abstract

The invention discloses a tissue-culture rapid propagation method for succulents. The method comprises the following steps: (1) selecting and sterilizing an explant: cutting tender leaves or stems of the succulents, and washing the cut leaves or stems cleanly; carrying out sterilizing treatment by adopting spirit and mercury bichloride, so as to obtain the explant; (2) carrying out primary culture: inoculating the explant obtained in the step (1) to a primary culture medium for primary culture until buds are differentiated from the explant; (3) carrying out propagation culture: cutting off the buds differentiated from the explant in the step (2), and inoculating the buds to a propagation culture medium for propagation culture until the length of a new bud germinated from each growth point is 3cm to 5cm and the multiplication coefficient is greater than 2; (4) carrying out rooting culture: separating new buds germinated in the step (3) into single plants, and inoculating the single plants to a rooting culture medium for rooting culture until each single plant grows 2 to 3 new roots and the length of each new root is greater than 3cm. According to the method, the culture time is short, the propagation frequency is high, young seedlings are regular, and the labor cost is reduced, so that the method can be used for carrying out industrialized production.

Description

technical field [0001] The invention relates to the technical field of biological group seedling cultivation, in particular to a method for tissue culture and rapid propagation of succulents. Background technique [0002] Succulentsrefers to a certain part of the plant's vegetative organs, such as stems or leaves or roots a few species have two parts, a type of plant that has developed parenchyma to store water, and appears plump and succulent in appearance. Most of them grow in arid areas or areas that are dry for a period of time in a year. For a long time every year, the roots cannot absorb water, and they only rely on the water stored in their bodies to maintain their lives. [0003] Small potted succulent plants are favored by consumers because they are easy to cultivate, have various overall shapes, colors and leaf shapes, and have high ornamental value. Some succulent plant manufacturers rely on leaf cuttings for propagation, which is not efficient, has low reproduct...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王小辉杨小霞朱晓菲沈香兰马良良马建华
Owner 四川大巴山生态农业开发有限公司
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