Winged euonymus stem adventitious bud inducing method

A technology of bud induction and stem segment, applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient, heavy workload and high cost

Inactive Publication Date: 2016-08-17
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to find a simple and feasible method for obtaining adventitious buds of U. Provide technical support for the establishment of the in vitro rapid propagation system of Euonymus euonymus and the mass production and supply of Euonymus seedlings

Method used

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  • Winged euonymus stem adventitious bud inducing method
  • Winged euonymus stem adventitious bud inducing method
  • Winged euonymus stem adventitious bud inducing method

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Experimental program
Comparison scheme
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Embodiment Construction

[0036] 1. Selection of explants:

[0037] Select euonymus plants that grow healthily in the wild, and at about 14:00 in the afternoon on a sunny day in mid-May, select a number of branches that have 3-5 internodes and grow well in the current year from plants that are 2-3 years old, and place them in a self-contained garden. Take it back to the laboratory in the bag.

[0038] 2. Pretreatment of explants:

[0039] (1) Cut off the leaves of the taken branches and cut them into 2cm stems;

[0040] (2) Put the cut stems in a beaker, cover them with gauze and put them under running water, rinse with the maximum water flow for 10 minutes to remove surface dirt;

[0041] (3) Soak the washed stems in washing powder water for 30 minutes, during which time brush the axillary buds of the stems with a brush to clean deeply;

[0042] (4) Remove the stem section from the washing powder water and transfer it to another beaker, place the gauze on it and rinse it under running water for 2 h...

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Abstract

The invention provides a winged euonymus stem adventitious bud inducing method. The winged euonymus stem adventitious bud inducing method comprises the following steps that an annual branch good in growth vigor with the length of 3-5 internodes is selected, and a stem with leaves pruned off is obtained; a washing powder solution and flow water are used for washing the stem clean; an explant is sterilized on an ultra-clean worktable with a series of sterilization methods such as ultraviolet lamp irradiation, and 75% alcohol and Tween in combination with mercury bichloride; the stem is vertically inserted into a culture medium with the base part of the stem facing downwards; the culture temperature is set to be 24+/-1 DEG C, the humidity is set to be 50-60%, the light period is 16 h of illumination, and the light intensity is set to be 2000-3000 lx; growth conditions are recorded. According to the winged euonymus stem adventitious bud inducing method, with the stem of winged euonymus serving as the explant, a winged euonymus stem sterile system is successfully established, and adventitious buds re successfully induced; besides, the inducing rate of adventitious buds reaches 70.67%, the average number of buds of each explant reaches 5.6, the propagation coefficient in the subsequent propagation process reaches 23.51, and the average bud length is 1.28 cm. The production cycle is short, and the propagation coefficient is high.

Description

technical field [0001] The invention relates to a plant tissue culture technique method, in particular to a method for inducing adventitious buds from euonymus stem segments. Background technique [0002] Euonymus alatus (Thunb.) Sieb. is a deciduous shrub of the genus Euonymus L. in the family Celastracea. It has a dense plant shape, purple-red autumn leaves, and orange-red arils. It is of great ornamental value and is an important wild foliage and fruit shrub. At the same time, the root and skin of Euonymus lanceolata can be used as medicine, which has the effects of breaking blood, killing insects, stimulating menstruation, dispelling fatigue and relieving pain, regulating blood lipids, lowering blood sugar, and delaying atherosclerosis. In recent years, it has been clinically used in the treatment of diabetes in my country. Significant results have been obtained. Euonymus is also mostly used to fight tumors in South Korea and Japan, and has good medicinal value. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 陆秀君赵丽蒙刘广林张晓林张丽杰孙晓梅王玉涛
Owner SHENYANG AGRI UNIV
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