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Rapid seedling propagation method for Chinese aralis

A technology of alder tree and seedlings, applied in the field of biotechnology application, can solve the problems of vegetation destruction, low survival rate of cuttings and seedlings, low natural reproduction rate of alder tree, etc.

Inactive Publication Date: 2015-01-07
HUZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aralia has a low natural reproductive rate and poor regenerative ability
Although Aralia can flower and set seeds, and can carry out sexual reproduction, the variation rate of seed reproduction is relatively large, the commerciality is reduced, and the reproduction rate is very low
In production, asexual reproduction is mainly used, and cuttings are propagated by branches or root segments, but this often results in waste of resources and damage to vegetation, and the survival rate of cutting seedlings is low and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] After washing the dormant buds of Aralia chinensis, soak them in 70% ethanol for 1 min, then sterilize them with 0.1% mercuric acid for 5 min, rinse the surface of the explants with sterile water, and cut them with a sterile scalpel on the ultra-clean workbench. The shoot tips were cut into small pieces with a thickness of about 0.5-1cm, placed on the medium MS+1.5mg / L 6-BA+0.01mg / L NAA for cultivation, and then the induced adventitious buds were inoculated on 1 / 2MS+0.5 On the rooting medium of mg / L IBA+0.01mg / L NAA, after about 4 weeks, about 1 to 3 new roots will grow and can be transplanted.

example 2

[0015] After washing the twig buds of Aralia chinensis, soak them in 70% ethanol for 1 minute, then disinfect them with 0.1% mercuric acid for 8 minutes, rinse the surface of the explants with sterile water, and use a sterile scalpel on the ultra-clean workbench. Cut the shoot tips into small pieces with a thickness of about 0.5-1cm, place them on the medium MS+1.0mg / L 6-BA+0.2mg / L IBA for culture, and then inoculate the induced adventitious buds on 1 / 2MS+0.5 mg / L IBM+0.2mg / L NAA+2g / L activated carbon rooting medium for rooting. After about 4 weeks, the seedlings will grow about 1 to 3 new roots and can be transplanted.

example 3

[0017] After washing the young buds of Aralia chinensis, soak them in 70% ethanol for 30 seconds, then disinfect them with 0.1% mercuric acid for 10 minutes, rinse the surface of the explants with sterile water, and cut them with a sterile scalpel on the ultra-clean workbench. The shoot tips were cut into small pieces with a thickness of about 0.5-1cm, placed on the medium MS+2.0mg / L6-BA+0.5mg / L 2-4D for cultivation, and then the induced adventitious buds were inoculated on 1 / 2MS+0.2 The rooting medium of mg / L 6-BA+0.5mg / L NAA is used for rooting. After about a month, the seedlings will grow about 1 to 3 new roots and can be transplanted.

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PUM

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Abstract

The invention relates to the field of biotechnology application and discloses a rapid seedling propagation method for Chinese aralis. The method comprises the following specific steps: 1, washing a top dormant bud of Chinese aralis, which is taken as an explants, by using running water, soaking by using 70 percent alcohol, disinfecting in a 0.1 percent mercury bichloride solution, and finally thoroughly rinsing by using sterile water; 2, cutting the disinfected explant into small blocks of 0.5-1cm, inoculating to a prepared induction medium for cultivation, and differentiating adventitious buds, wherein the culture conditions are as follows: the light intensity is 1500-20001x, the temperature is 25+ / -1 DEG C and the illumination is 12 hours; 3, transferring the induced adventitious buds to a subculture medium for performing enrichment culture, so that bud seedlings are healthy and strong; and 4, inoculating the differentiated adventitious buds into a rooting medium, and transplanting when the adventitious roots grow out, wherein the survival rate of the transplanted seedlings can be over 80 percent. The method disclosed by the invention is easy and feasible to operate, high in propagation speed and suitable for industrialized seedling production.

Description

Technical field: [0001] The invention relates to a biotechnology application field, and relates to a method for rapid propagation of aralia seedlings by utilizing plant tissue in vitro culture technology. Background technique: [0002] Aralia is a deciduous shrub or a small tree of Araliaceae. Branchlets sparsely spiny, covered with yellow tomentose. 2 to 3 pinnate compound leaves alternate; pinnae with 5 to 13 leaflets, and a pair of leaflets at the base; leaflets ovate, broadly ovate or ovate-elliptic, apex acuminate or short acuminate, base round Or subcordate margin finely serrate. The umbels form terminal large panicles, the main axis is more than 30cm long, and the primary branches are arranged in racemes on the main axis, the pedicel is obvious, and the flowers are white; the fruit is spherical, with 5 edges, black or purple black when ripe. The flowering period is June-August, and the fruiting period is September-October. Produced in all parts of the country, bor...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈丽萍鱼南洋张志友韩明丽赵根李艳冬谭宏胡亮
Owner HUZHOU ACAD OF AGRI SCI
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