Preservation method of fertilized eggs and embryos of sargassum fusiforme

A preservation method and fertilized egg technology, applied to plant cells, cells modified by introducing foreign genetic material, fusion cells, etc., can solve problems such as difficulties in actual production, and achieve the effects of saving trouble, delaying the development process, and low fertilization rate

Inactive Publication Date: 2010-06-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this method will bring a lot of difficulties to actual production when it is necessary to comp...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0017] 1) Collection of eggs and sperms released simultaneously from Hijiki: the male and female parents of Hijiki were cultured under the conditions of light intensity of 50,000 lux, light cycle of 16 hours, and temperature of 24 degrees, and the water was changed once within 24 hours, and cultured for 8 Days, so that the parents realize the synchronous discharge of eggs and sperm, and collect them within 24 hours after the parents release the eggs and sperm synchronously; the male to female ratio of the male and female parents of Sargassum hijiki is 10:1.

[0018] 2) Preservation: fertilize the collected eggs and sperm that are released synchronously, and the ratio of the eggs and sperm that are released synchronously is 1:100. Within 16 hours after fertilization, complete the collection of fertilized eggs and embryos at a low temperature of 8°C, and at the same time store the collected fertilized eggs and embryos in a nutrient solution at a low temperature of 8°C and 0 lux; ...

Embodiment 3

[0020] 1) Collection of eggs and sperms released simultaneously from Hijiki: culture the male and female parents of Hijiki under the conditions of light intensity of 30,000 lux, light cycle of 12 hours, and temperature of 20 degrees, and change the water once within 24 hours, and cultivate for 10 days. Days, so that the parents realize the synchronous discharge of eggs and sperm, and collect them within 20 hours after the parents synchronously release eggs and sperm; the male and female ratio of the male and female parents of Sargassum hijiki is 10:1.

[0021] 2) Preservation: Fertilize the collected eggs and sperm that are released synchronously, and the ratio of the eggs and sperm that are released synchronously is 1:50. Within 10 hours after fertilization, complete the collection of fertilized eggs and embryos at a low temperature of 1°C, and at the same time store the collected fertilized eggs and embryos in a nutrient solution at a low temperature of 1°C and 200 lux; the c...

Embodiment 4

[0023] 1) Collection of eggs and sperms released simultaneously from Hijiki: the male and female parents of Hijiki were cultured under the conditions of light intensity of 40,000 lux, light cycle of 14 hours, and temperature of 22 degrees, and the water was changed once within 24 hours, and cultured for 6 Days, so that the parents realize the synchronous discharge of eggs and sperm, and collect them within 24 hours after the parents release the eggs and sperm synchronously; the male to female ratio of the male and female parents of Sargassum hijiki is 10:1.

[0024] 2) Preservation: Fertilize the collected eggs and sperm that are released synchronously, and the ratio of the eggs and sperm that are released synchronously is 1:10-100. Complete the collection of fertilized eggs and embryos at a low temperature of 5°C within 14 hours after fertilization, and simultaneously store the collected fertilized eggs and embryos in a nutrient solution at a low temperature of 5°C and 50 lux;...

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Abstract

The invention relates to the rearing of economic brown algae, in particular to a preservation method of fertilized eggs and embryos of sargassum fusiforme, which specifically comprises the following steps: culturing male and female parents of sargassum fusiforme under the conditions that the light intensity is 10000-50000 lux, the photoperiod is 12-16 hours, and the temperature is 18-24 DEG C, replacing water within 24 hours once, and culturing for 7-10 days, so that the parents synchronously discharge eggs and sperms; and collecting the fertilized eggs within 12 to 24 hours after the parents synchronously discharge the eggs and the sperms, completing the collection of the fertilized eggs and the embryos at a temperature of 0 to 8 DEG C within 8-16 hours after fertilization, and then preserving. The invention utilizes the low-temperature method to culture the eggs in an appropriate culture medium within 8-16 hours after the sargassum fusiforme eggs are fertilized, and then preserves the fertilized eggs and the embryos, thereby substantially delaying growth process, so that the cross-regional transport is completed before rhizoids are not formed so as to carry out seed rearing.

Description

technical field [0001] The invention relates to the cultivation of economical brown algae, in particular to a method for preserving fertilized eggs and embryos of hijiki. Background technique [0002] Hijiki is a dioecious economic brown algae with low fertilization rate and asynchronous development. At present, the method of fully artificial seed cultivation is usually adopted. This method uses mature parents to grow vegetables, or individuals taken from natural populations. Either artificially cultivated individuals, or the synchronous maturation of male and female parents realized by biotechnology in the artificial cultivation system, collect fertilized eggs, and complete the process of planting seedlings. These technical methods need to finish the cloth seedling process rapidly after collecting fertilized eggs, so that fertilized eggs can be attached to the attachment base when rhizoids are produced. Therefore, when it is necessary to complete cross-region seedling cult...

Claims

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Application Information

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IPC IPC(8): C12N5/14C12N5/04
Inventor 逄少军张志怀张玉荣
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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