In-vitro preservation method for sargassum fusiforme rhizoid germplasm resources

A technology of in vitro preservation and vegetable preparation, which is applied in botany equipment and methods, horticulture, agriculture, etc., can solve the problems that there are no successful cases of long-term preservation, and achieve the effects of long preservation time, resource saving and simple operation

Active Publication Date: 2022-02-18
WENZHOU UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research on the preservation methods of indoor germplasm resources of Hijiki mainly includes ultra-low temperature and low-temperature preservation of fertilized eggs...

Method used

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  • In-vitro preservation method for sargassum fusiforme rhizoid germplasm resources
  • In-vitro preservation method for sargassum fusiforme rhizoid germplasm resources
  • In-vitro preservation method for sargassum fusiforme rhizoid germplasm resources

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Embodiment 1

[0023] A method for preserving germplasm resources by rhizomes of Hijiki is characterized in that it comprises the following steps:

[0024] (1) Screening and cleaning of hijiki rhizomes:

[0025] After the Hijiki is harvested, take the rhizome attached to the seedling rope, remove the algae, molluscs, sludge and other attachments attached to the rhizome, and wash it with seawater after filtration and high-pressure steam sterilization for 3-4 times. Choose healthy and undamaged hijiki rhizomes as preservation materials.

[0026] (2) Pretreatment of hijiki rhizome:

[0027] Soak the selected hijiki rhizomes in a solution of 0.38% NaClO by volume fraction and 0.5% KI by mass fraction for 3 minutes for disinfection, and then rinse them quickly with sterilized seawater for 3-4 times. The rhizome of Hijiki was placed at a temperature of 20 °C and a light intensity of 84 μmol photons m -2 ·s -1 1. Under the condition that the light-dark cycle ratio is 12L:12D and the seawater sa...

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Abstract

The invention discloses an in-vitro preservation method for sargassum fusiforme germplasm resources, and mainly relates to a low-temperature and low-salinity preservation method taking sargassum fusiforme rhizoid as a germplasm preservation material. The method is characterized in that long-time in-vitro preservation of the sargassum fusiforme rhizoid under the conditions that the temperature is 8-10 DEG C and the salinity is 19-20%, and the growth rate and the germination rate of the sargassum fusiforme rhizoid in the preservation period are effectively inhibited. The preservation method is easy to operate, resources are saved, and survival and germination of the sargassum fusiforme rhizoid are not affected; besides, normal growth, germination and seedling formation of the sargassum fusiforme rhizoid can be recovered under the proper culture condition, so that the key problem that sargassum fusiforme indoor germplasm resources are difficult to preserve is effectively solved, the preservation time can reach 4 months or above, the rhizoid germination rate can reach 400% or above after recovery culture, and the seedling rate can reach 150% or above.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for preserving germplasm resources in vitro by rhizomes of hijiki. Background technique [0002] Sargassum fusiforme is a large brown algae with high nutritional, medicinal, ecological and economic value. my country is one of the main countries in the world where hijiki is cultivated, and hijiki cultivation has important economic and ecological significance. [0003] In the entire life cycle of Hijiki, except for the breeding period of artificial sexual reproduction, which is carried out in nursery ponds, the rest of the life cycle is completed in sea areas, so there is a risk of loss of excellent germplasm resources under extreme climate conditions , such as typhoon loss and high temperature rot, etc.; there is also the risk of loss of traits of excellent lines due to genetic drift caused by sexual reproduction and breeding. Based on the above reasons, the long-te...

Claims

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Application Information

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IPC IPC(8): A01G33/00
CPCA01G33/00Y02A40/80
Inventor 吴明江陈斌斌徐丽丽林立东马增岭罗琳左晓洁
Owner WENZHOU UNIVERSITY
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