A kind of induction method of Sargassum regeneration plant

Abstract

The invention discloses an induction method of gulfweed regeneration plant. The induction method of the gulfweed regeneration plant comprises the following steps: preparing a sterile explant, inoculating the explant to an induction culture medium and inducing callus to form at an induction temperature of 15-20 DEG C with a light intensity of 4000-5000Lx and a light period of 8 hours; processing gulfweed rhizoid in 0.2% povidone for 3 minutes, and then sterilizing in a 3% sodium hypochlorite solution for 5 minutes, sterilizing in 0.1% mercury bichloride for 3 minutes so as to obtain a sterile survival explant; adding 0.1-1mg of ZT, 1-2mg of IAA (Indole Acetic Acid), 1-3g of ascorbic acid, 30g of cane sugar and 7g of agar in a 1LPES culture medium, sterilizing at a high temperature and a high pressure for 10-20 minutes to bud the gulfweed.

Description

technical field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Sargassum. Background technique

[0002] Sargassum is a large-scale economical brown algae with a maximum length of more than 7m. The natural algae field formed by Sargassum contains rich biological resources. It is a good breeding place for fish, shrimp and crabs, and is the ecology with the highest marine productivity. one of the systems. Sargassum is rich in carbohydrates, lipids, proteins, vitamins, minerals and a variety of trace elements necessary for the human body, and its development and utilization prospects are very broad. Sargassum is a perennial seaweed. Due to its fast growth rate, large biomass and important ecological service functions in shallow sea ecological environments, it can be used as a "blue carbon sink", algae farm reconstruction, ecosystem environmental restoration, artificial fish reefs and It is one of the...