Asexual propagation method of liverwort

A land money and tissue block technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of troublesome movement, large space occupation, low breeding efficiency, etc., and achieve the effect of saving space and cost

Inactive Publication Date: 2012-07-11
LIYANG AOGU INFORMATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this method needs to prepare flowerpots, soil, etc., on the one hand, it takes up a lot

Method used

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  • Asexual propagation method of liverwort
  • Asexual propagation method of liverwort
  • Asexual propagation method of liverwort

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Prepare MS liquid medium. The specific formula is as follows:

[0032] Potassium nitrate 1900mg / l, ammonium nitrate 1650mg / l, potassium dihydrogen phosphate 170mg / l, magnesium sulfate 370mg / l, calcium chloride 440mg / l, potassium iodide 0.83mg / l, boric acid 6.2mg / l, manganese sulfate 22.3mg / l l, Zinc sulfate 8.6mg / l, sodium molybdate 0.25mg / l, copper sulfate 0.025mg / l, cobalt chloride 0.025mg / l, disodium EDTA 37.3mg / l, ferrous sulfate 27.8mg / l l, inositol 100mg / l, glycine 2mg / l, thiamine hydrochloride 0.1mg / l, pyridoxine hydrochloride 0.5mg / l, niacin 0.5mg / l.

[0033] (2) Obtain cultured tissue blocks

[0034] From the ground money (M.polymorpha). The thallus of Dictonias was cut into pieces of 2 × 2 mm. Use a coarse-grained soil sieve to screen out too small tissue pieces.

[0035] (3) Sterilization of tissue blocks

[0036] First, wash the cut tissue pieces with tap water, then soak them in 70% alcohol for 1 minute on the ultra-clean bench, then sterilize the...

Embodiment 2

[0042] (1) Prepare B5 liquid medium. The specific formula is as follows:

[0043] Potassium nitrate 2500mg / l, magnesium sulfate 250mg / l, calcium chloride 150mg / l, ammonium sulfate 134mg / l, potassium iodide 0.75mg / l, boric acid 3.0mg / l, manganese sulfate 10mg / l, zinc sulfate 2.0mg / l, Sodium molybdate 0.25mg / l, cobalt chloride 0.025mg / l, copper sulfate 0.025mg / l, disodium EDTA 37.3mg / l, ferrous sulfate 27.8mg / l, inositol 100mg / l, cigarette smoke Acid 1.0mg / l, pyridoxine hydrochloride 1.0mg / l, ammonium sulfate hydrochloride 10mg / l.

[0044] (2) Obtain cultured tissue blocks

[0045]It is based on the ground money (M. cuneiloba). Cut the fronds of the ground money into 2×2mm tissue pieces. Use a coarse-meshed soil sieve to screen out tissue pieces that are too small.

[0046] (3) Sterilization of tissue blocks

[0047] First, wash the chopped tissue pieces with tap water, then soak them in 70% alcohol on the ultra-clean bench for 1 minute, and then sterilize them with 0.1% m...

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Abstract

The invention provides a novel method for propagating liverwort. According to the method, the liverwort is propagated by using a liquid culture medium; a plastic bracket is arranged in the liquid culture medium, so that tissue blocks of the liverwort cannot be immersed in the culture medium; and the tissue blocks of the liverwort are managed respectively before or after the rhizoids grow, and therefore, the aim of the rapid and efficient propagation of the liverwort is achieved.

Description

technical field [0001] The present invention relates to a breeding method of A. chinensis, in particular to a vegetative breeding method of A. chinensis. Background technique [0002] Diqian belongs to bryophytes, from the perspective of plant taxonomy, it belongs to Bryophyta, Moss, Diqian, Diqianaceae, Diqian. [0003] Diqian is used as medicine as a whole, has a mild taste and is cool in nature, and enters the spleen and lung meridians. It has the functions of dispelling heat, astringent, nourishing qi, clearing tonic and improving eyesight. It can treat fire eye night blindness, scald burns, lung heat cough and chronic dysentery prolapse of the rectum. Scientists from Weizmann Research in Israel have found that a component contained in the earth money can inhibit the activity of acetylcholinesterase in the human brain, which can have a curative effect on Alzheimer's disease. [0004] When used as food, it has significant therapeutic effects: [0005] 1. Reduce lipids...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杜春林
Owner LIYANG AOGU INFORMATION TECH
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