A seedling raising method for inducing synchronous maturation of isolated reproductive trays of Sargassum leizhou

A technology for reproductive care and sargassum, which is applied in the fields of botanical equipment and methods, seaweed cultivation, climate change adaptation, etc. It can solve the problems of difficulty in determining the number of female and male algal bodies, uneven light exposure of larvae, and difficulty in large-scale seedling raising. , to achieve the effect of improving fertilization rate and emergence rate, conducive to simultaneous maturation and low cost

Inactive Publication Date: 2016-09-28
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to determine the number of female and male algae recovered by this method, and it is difficult to raise seedlings on a large scale. At the same time, the temporary breeding time is relatively long, the water consumption is large, the management is inconvenient, the larvae are unevenly exposed to light, and they are easy to be polluted.
Application No. 201310296487.8 "A Method for Tissue Culture and Seedling Cultivation of Sargassum varsonii" solves the problem of asexual reproduction and seedling cultivation of Sargassum varmannii

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A seedling raising method for inducing the synchronous maturation of Sargassum leizhou isolated reproductive trays, comprising the following steps:

[0024] (1) Collection and transportation of Sargassum Leizhou algae: In winter or spring when Leizhou Sargassum is immature, select male and female algae that are robust, less miscellaneous, more side branches, and uniform in maturity during high tide and low tide. Put it into the sorting box and transport it to the destination by wet transport method.

[0025] (2) Temporary cultivation of algae: For the Sargassum algae transported back, the female and male algae are temporarily raised in seawater filtered with a 50 μm filter. Temporary cultivation conditions: water temperature 20°C, salinity 26, light intensity 60 μmol m -2 the s -1 In between, inflate the water to make the water boil and roll.

[0026] (3) Pretreatment of algae: Clean the surface of algae with filtered seawater to remove miscellaneous algae and attach...

Embodiment 2

[0031] A method for inducing maturation of the reproductive receptacle of Sargassum Leizhou, comprising the steps of:

[0032] (1) Collection and transportation of Sargassum Leizhou algae: In winter or spring when Leizhou Sargassum is immature, select male and female algae that are robust, less miscellaneous, more side branches, and uniform in maturity during high tide and low tide. Put it into the sorting box and transport it to the destination by wet transport method.

[0033] (2) Temporary cultivation of algae: For Sargassum algae transported back, the female and male algae are temporarily raised in seawater filtered with a 50 μm filter. Temporary cultivation conditions: water temperature 22°C, salinity 28, light intensity 10μmol m -2 the s -1 In between, inflate the water to make the water boil and roll.

[0034] (3) Pretreatment of algae: Clean the surface of algae with filtered seawater to remove miscellaneous algae and attachments.

[0035] (4) Separation of the rep...

Embodiment 3

[0039] A method for inducing maturation of the reproductive receptacle of Sargassum Leizhou, comprising the steps of:

[0040] (1) Collection and transportation of Sargassum Leizhou algae: In winter or spring when Leizhou Sargassum is immature, select male and female algae that are robust, less miscellaneous, more side branches, and uniform in maturity during high tide and low tide. Put it into the sorting box and transport it to the destination by wet transport method.

[0041] (2) Temporary cultivation of algae: For Sargassum algae transported back, the female and male algae are temporarily raised in seawater filtered with a 50 μm filter. Temporary cultivation conditions: water temperature 22°C, salinity 28, light intensity 50 μmol m -2 the s -1 In between, inflate the water to make the water boil and roll.

[0042] (3) Pretreatment of algae: Clean the surface of algae with filtered seawater to remove miscellaneous algae and attachments.

[0043] (4) Separation of the re...

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Abstract

The invention relates to a seedling raising method for inducing synchronous maturation of isolated reproductive trays of Sargassum leizhou, belonging to the technical field of algae breeding. Collect non-ovulated and non-ovulated Sargassum leizhou algae, separate and obtain in vitro reproductive receptacle with female receptacle length 4~5mm and male receptacle length 7~15mm, at temperature 23~30℃, salinity 34, light intensity 80μmol· m‑2s‑1, photoperiod 12L:12D to promote maturation; collect fertilized eggs that have naturally fallen off and have just grown papillary rhizomes, at a temperature of 23°C, a salinity of 30~34, and a light intensity of 80 μmol·m‑ 2s-1, under the condition of photoperiod 12L:12D, static culture for 15 days to obtain seedlings with a length of 5.0~5.7mm. The present invention changes the traditional method of harvesting seedlings together with the algae, and separates the reproductive trays into individual or single clusters to promote maturation and cultivation, which is more targeted and is conducive to the simultaneous maturation of the reproductive trays and the concentrated discharge of a large number of sperm and eggs. The female and male reproductive receptacles can reach 100% maturity, which reduces the pollution from algae and the waste of algae resources during seedling cultivation, improves the fertilization rate and emergence rate, and the seedlings grow fast and have a high survival rate, and the method is simple.

Description

technical field [0001] The invention relates to a seedling raising method for inducing the synchronous maturation of isolated reproductive trays of Sargassum leizhou, in particular to a method for separating immature reproductive trays of Sargassum leizhou and cultivating them under certain conditions to realize the simultaneous maturation of male and female reproductive trays of Sargassum leizhou. The invention discloses a method for raising seedlings by collecting fertilized eggs, belonging to the technical field of algae breeding. Background technique [0002] Leizhou Sargassum Sargassum leizhouense Tseng et Lu (1987) is a unique species of brown algae Sargassum in my country. It is large in size and fast growing. It is mainly distributed in the waters of Guangdong and Hainan in my country, and grows on rocks in the low tide zone and subtidal zone. Leizhou Sargassum is currently mainly fed to abalone, or used to extract raw materials such as alginate, mannitol and iod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 赵素芬孙会强
Owner GUANGDONG OCEAN UNIVERSITY
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