Cultivation method of collybia albuminosa mother strain

A culture method, the technology of the chicken fir fungus, applied in the cultivation field of the female species of the black-skinned chicken fir, can solve the problems of low yield, poor quality, slow mycelium growth rate, etc., to improve the quality of the female species, improve the quality, and promote The effect of mycelial growth

Inactive Publication Date: 2018-04-24
四川山水美地农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the problems existing in the prior art, the object of the present invention is to provide a kind of cultivation method of the female species of Gallus fir fruticosa that can promote the growth of mycelia and...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of cultivating method of the female species of P. nigriti, comprising the following steps:

[0018] Step 1): Under aseptic conditions, the fruiting body of Gallus pilosulae is separated from the tissue, and the fruiting body mycelium is separated;

[0019] Step 2): performing tissue separation on the rhizoids of Gallinarum nigritiformis, and separating the rhizoid mycelia;

[0020] Step 3): Transfer the fruiting mycelium to a test tube with a culture medium, culture at 23°C, insert the rhizoid mycelium when the mycelium grows to 1.3-1.8 cm, and continue culturing for 6 days to mature Mother species;

[0021] The medium components are: 1000ml of potato juice, 20g of agar, 20g of glucose, 3g of organic acid salt, and 20ml of symbiotic bacteria liquid.

[0022] The preparation method of the medium is as follows: put 100 g of potatoes into 1000 ml of water to heat, continue heating for 25 minutes after boiling, filter while it is hot, add water to the filtrate to m...

Embodiment 2

[0025] A kind of cultivating method of the female species of P. nigriti, comprising the following steps:

[0026] Step 1): Under aseptic conditions, the fruiting body of Gallus pilosulae is separated from the tissue, and the fruiting body mycelium is separated;

[0027] Step 2): performing tissue separation on the rhizoids of Gallinarum nigritiformis, and separating the rhizoid mycelia;

[0028] Step 3): transfer the mycelium of the fruiting body to a test tube with a culture medium, cultivate it at 25°C, insert the mycelium of the rhizome when the mycelium grows to 1.3-1.8cm, and continue culturing for 5 days to obtain mature mother species;

[0029] The medium components are: 1000ml of potato juice, 20g of agar, 20g of glucose, 5g of organic acid salt, and 25ml of symbiotic bacteria liquid.

[0030] The preparation method of the medium is as follows: put 100 g of potatoes into 1000 ml of water to heat, continue heating for 30 minutes after boiling, filter while it is hot, ...

Embodiment 3

[0033] A kind of cultivating method of the female species of P. nigriti, comprising the following steps:

[0034] Step 1): Under aseptic conditions, the fruiting body of Gallus pilosulae is separated from the tissue, and the fruiting body mycelium is separated;

[0035] Step 2): performing tissue separation on the rhizoids of Gallinarum nigritiformis, and separating the rhizoid mycelia;

[0036] Step 3): Transfer the fruiting body mycelium to the Erlenmeyer flask containing the culture medium and shake it at a temperature of 23°C for cultivation. When a small amount of mycelial balls are suspended in the liquid, insert the rhizoid mycelium and continue the cultivation After 5 days, a large number of small mycelium balls were suspended in the liquid to obtain the liquid mother species.

[0037] The medium components are: 1000ml of potato juice, 20g of agar, 20g of glucose, 4g of organic acid salt, and 15ml of symbiotic bacteria solution.

[0038] The preparation method of the...

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PUM

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Abstract

A cultivation method of a collybia albuminosa mother strain comprises the following steps that firstly, fruiting bodies of collybia albuminosa are subjected to tissue separation under aseptic conditions, and fruiting body mycelia are obtained after separation; secondly, rhizoid of collybia albuminosa is subjected to tissue separation, and rhizoid mycelia are obtained after separation; thirdly, thefruiting body mycelia are transferred into a test tube containing a culture medium for cultivation, after the mycelia grow to 1.3-1.8 cm, the mycelia are inoculated into the rhizoid mycelia, and cultivation continues to obtain the mature mother strain; or the fruiting body mycelia are transferred into a triangular flask containing the culture medium for shake cultivation, the rhizoid mycelia areinoculated when a small quantity of mycelium pellets suspend in liquid, cultivation continues, and the liquid mother strain is obtained after a large quantity of small mycelium pellets suspend in theliquid. One of the two modes is selected for cultivation. The mycelia biomass of the mother strain cultivated through the cultivation method is 2.3 g/100 ml or above, the mycelia are dense and strong,and the color of the mycelia is a little amber.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for cultivating the female species of Gallinarum nigricans. Background technique [0002] Gallium fir fungus has high edible and medicinal value, and it is also one of the traditional medicinal fungi in my country. It is a famous wild edible fungus because of its tender and mellow aroma, tender meat, white as jade, unique taste, edible both raw and cooked, dual-purpose food and medicine, and rich nutrition. Now medical research has found that the Gallus pilosula contains ergosterol substances and active ingredients for treating diabetes, which have obvious effects on lowering blood sugar and inhibit the growth of human cancer cells. Therefore, from ancient times to the present, the Gallus fir fungus is a best-selling species in the market, and the most popular with the black-skinned Gallus fir fungus. [0003] Although P. nigritiformis is ...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
Inventor 吴玲周善龙
Owner 四川山水美地农业投资有限公司
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