Termitomyces albuminosus with black skin producing process
A production method, the technology of Gallus pilosula, is applied in the direction of botany equipment and method, fertilizer mixture, gardening, etc., to achieve the effect of reducing impact, simple production process and low production cost
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Embodiment 1
[0050] 1.1 Preparation of the female species (combined culture with the fruiting bodies and rhizoids of the black-skinned fungus collected in Chuxiong, Yunnan in 2004):
[0051] 1.1.1 Medium formula:
[0052] Potato juice 1000ml, agar 30g, glucose 20g.
[0053] 1.1.2 Preparation steps:
[0054] a. Prepare culture medium according to the above formula
[0055] Put 200g of potatoes into 1000ml of water to heat, boil and continue to heat for 20 minutes, filter while it is hot, add water to the filtrate to make up 1000ml, then add the formula amount of agar and glucose. After the agar and glucose are completely melted, divide into test tubes to obtain the mother culture medium;
[0056] B, in the triangular flask under aseptic condition, do spore separation to the sporocarp of P. nigeri, separate to obtain sporocarp mycelium; do tissue separation to the rhizoid of P. nigricans, separate to obtain pseudorhizomycelium body;
[0057] c. Transfer the fruiting mycelium to a test t...
Embodiment 2
[0081] 2.1 Preparation of female species (collected from Chuxiong Prefecture, Yunnan Province in the autumn of 2002, and preserved in a refrigerator at 3-5°C with conventional liquid paraffin, and the rhizoid rhizomes of Gallium fruticosa collected from Chuxiong Prefecture, Yunnan Province in 2004 For combined cultivation):
[0082] 2.1.1 Medium formula:
[0083] Jujube juice 1000ml, agar 25g, glucose 25g
[0084] 2.1.2 Preparation steps:
[0085] a. Prepare culture medium according to the above formula
[0086] Put 250g of jujube branches into 1000ml water and heat, boil and continue heating for 25 minutes, filter while it is hot, add water to the filtrate to make up 1000ml, then put agar and glucose. After the agar and glucose are completely melted, divide into test tubes to obtain the mother seed medium. ;
[0087] B, in the triangular flask under aseptic condition, do spore separation to the sporocarp of P. nigeri, separate to obtain sporocarp mycelium; do tissue sepa...
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