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A kind of cultivating method of P. nigella

A culturing method and technology for black-skinned chickens, which are applied in the field of culturing A. black-skinned chickens, can solve problems such as inability to realize artificial culture, and achieve the effects of short cycle, low production cost and high output.

Active Publication Date: 2019-02-12
金乡县联盛菌业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is far from being able to satisfy the huge market demand by collecting wild P. nigricans, and there is an urgent need to carry out large-scale artificial cultivation to it, and large-scale artificial cultivation cannot be realized so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.1 Preparation of mother species

[0026] 1.1.1 Culture medium composition

[0027] Wheatgrass juice 1000ml, agar 15g, sucrose 25g.

[0028] 1.1.2 Preparation of medium

[0029] Take 200g of wheat straw, add 1000ml of distilled water, simmer for 30-40min, filter out the juice after cooling, add 25g of sucrose and 15g of agar to the juice, heat to dissolve, add distilled water to make up to 1000ml, the pH of the solution is 6-7, pack it separately That is, the mother seed medium is obtained.

[0030] 1.1.3 Preparation process of mother species

[0031] a. under aseptic conditions, spore separation is performed on the sporocarp of P. nigeri to obtain sporocarp mycelium;

[0032] b. Transfer the fruiting mycelium into a test tube filled with culture medium, and culture at 19-21° C. for 10-15 days to obtain mature female species.

[0033] 1.2 Preparation of original species

[0034] 1.2.1 Culture medium composition

[0035] Wheat straw 65%, chestnut shell 32%, limes...

Embodiment 2

[0053] 2.1 Preparation of mother species

[0054] 2.1.1 Culture medium composition

[0055] Wheatgrass juice 1000ml, agar 15g, sucrose 25g.

[0056] 2.1.2 Preparation of medium

[0057] Take 200g of wheat straw, add 1000ml of distilled water, simmer for 30-40min, filter out the juice after cooling, add 25g of sucrose and 15g of agar to the juice, heat to dissolve, add distilled water to make up to 1000ml, the pH of the solution is 6-7, pack it separately That is, the mother seed medium is obtained.

[0058] 2.1.3 Preparation process of mother species

[0059] a. under aseptic conditions, spore separation is performed on the sporocarp of P. nigeri to obtain sporocarp mycelium;

[0060] b. Transfer the fruiting mycelium into a test tube filled with culture medium, and culture at 19-21° C. for 10-15 days to obtain mature female species.

[0061] 2.2 Preparation of original species

[0062] 2.2.1 Culture medium composition

[0063] Sorghum straw 8%, wheat straw 61%, chestnu...

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PUM

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Abstract

The invention discloses a culture method of black termitomyces albuminosus. The culture method comprises following steps of preparation of stock culture, preparation of stock seeds, preparation of production seeds and artificial cultivation. The salient characteristic of the method is that stock culture is prepared by hyphae of fruiting bodies of black termitomyces albuminosus; the culture medium used during culture is prepared by wheat straw juice, agar and cane sugar in a method; raw material is cheap and available; production cost is low; the period is short; output is high; and therefore, large-scale artificial cultivation of black termitomyces albuminosus is achieved so that the market needs of people for black termitomyces albuminosus are satisfied.

Description

technical field [0001] The invention relates to the field of fungus cultivation, in particular to a method for cultivating Gallus firjiri. Background technique [0002] P. nigeri is a nutrient-rich Edible fungus , it is rich in a variety of amino acids, and is one of the edible fungi that can be used for both medicine and food. It not only has the functions of benefiting the stomach, clearing the mind, curing hemorrhoids and lowering blood fat, but also has a unique taste and freshness, far surpassing monosodium glutamate In fact, its content of sodium glutamate is relatively high, and its taste is very fresh no matter it is fried, fried, boiled, deep-fried, steamed or made into soup. Although the nutritional value of P. nigriscens is very high, P. nigris in the natural state is only produced in the southwest and southeast regions of my country, and the requirements for the growth environment are extremely harsh. It only grows on the edge of termite nests. The nest is a cul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00
Inventor 张恩建
Owner 金乡县联盛菌业科技有限公司
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