Cultured Ganoderma yunnanense fruiting bodies and culture method thereof

A white ganoderma lucidum seed, artificial cultivation technology, applied in botanical equipment and methods, fertilizer mixtures, horticulture and other directions, can solve problems such as destruction of wild resources, difficulty in collection, etc., and achieve the effects of low cost, easy realization, and sustainable utilization.

Active Publication Date: 2012-07-11
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yunnan white ganoderma is the top grade of ganoderma. The amount of Yunnan white ganoderma that can be collected in Yunnan Province is only a few hundred kilograms each year, and it is becoming more and more difficult to collect. Rare things are more

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of mother species:

[0022] (1) Using the spores and fruiting bodies of Ganoderma lucidum obtained in the wild, the fruiting strains of Ganoderma lucidum were obtained through single-spore hybridization technology, and then systematically selected through the separation of fruiting body tissues. After 3 to 5 years of natural systematic breeding, the Breed into the original mother species of Yunnan Bailingzhi with strong stability, high metabolic capacity and high safety;

[0023] (2) To prepare and expand the parent species, add PDA medium to test tubes or other glass containers, sterilize and cool down, insert the original parent species of Ganoderma lucidum for cultivation, and cultivate the mycelia at 15-28°C for 10-15 days Overgrown on the surface of the medium to become a mature mother species, which can be used to reproduce the original species;

[0024] 2. Preparation of original species and cultivated species:

[0025] (1) The composition of med...

Embodiment 2

[0036] 1. The preparation of the mother species is the same as in Example 1.

[0037] 2. Preparation of original species and cultivated species:

[0038] (1) The composition of medium raw materials is (weight percentage, the same below)

[0039] 50% corn stalks, 40% sawdust, 8% bran, 1% calcium carbonate, and 1% sugar are used as raw materials, add water to make the water content 60% to 68%, and use lime powder to adjust the pH value to 7.0 to 8.5 to obtain the original species or cultivation kind of culture medium;

[0040] (2) Same as Example 1.

[0041] 3. Annual cultivation of Yunnan Bailingzhi:

[0042] (1) Preparation of medium:

[0043] 55% sawdust, 15% corn cob, 10% wheat bran, 7% rice bran, 7% corn flour, 2% fruiting body stimulator, 1% sugar, 1% superphosphate, 1% calcium carbonate, 0.5 potassium dihydrogen phosphate %, calcium sulfate 0.5% to form a solid material, add water to make the water content 60% to 65%, and use lime powder to adjust the pH value to 7.0 t...

Embodiment 3

[0047] 1. The preparation of the mother species is the same as in Example 1.

[0048] 2. Preparation of original species and cultivated species:

[0049] (1) The composition of medium raw materials is (weight percentage, the same below)

[0050] Straw 40%, sawdust 50%, bran 8%, calcium carbonate 1%, sugar 1% as raw materials, add water to make the water content 60% ~ 68%, use lime powder to adjust the pH value to 7.0 ~ 8.5, to obtain the original species or cultivation kind of culture medium;

[0051] (2) Same as Example 1.

[0052] 3. Annual cultivation of Yunnan Bailingzhi:

[0053] (1) Preparation of medium:

[0054] 10-30 cm long section wood 85%, wheat bran 5%, rice bran 2%, corn flour 2%, fruiting body stimulator 2%, sugar 1%, superphosphate 1%, calcium carbonate 1%, dihydrogen phosphate Potassium 0.5% and calcium sulfate 0.5% form a solid material, add water to make the water content 50% to 55%, and use lime powder to adjust the pH value to 7.0 to 8.5 to obtain a c...

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PUM

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Abstract

The invention relates to cultured Ganoderma yunnanense fruiting bodies and a culture method of the fruiting bodies. The culture method comprises the following steps: screening Ganoderma yunnanense spores and fruiting bodies obtained from field by single spore hybridization technique to obtain Ganoderma yunnanense fruiting strains; performing systematic breeding through tissue isolation of fruiting bodies; performing natural systematic breeding for 3-5 years to obtain Ganoderma yunnanense original mother cultures with strong stability, high metabolic capacity and high safety; and preparing mother cultures, preparing stock seeds and cultivated species, and culturing for one year with log, sawdust, wooddust and crop straws and other leftovers of agriculture and forestry industry as main culture raw materials, and a fruiting body activating agent, wheat bran, rice bran, corn meal, sugar and mineral elements as a culture medium in natural environment conditions while manually controlling temperature, humidity, air and illumination to obtain Ganoderma yunnanense fruiting bodies. The method of the invention is low in cost, easy to implement, can be widely applied for large-scale production of Ganoderma yunnanense, and realizes sustainable utilization of natural resources of Ganoderma yunnanense.

Description

technical field [0001] The invention relates to the technical field of domestication and cultivation of rare wild fungi, in particular to an artificially domesticated and cultivated Yunnan ganoderma lucidum fruiting body and a cultivation method thereof. Background technique [0002] Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine in the motherland, and is known as the "immortal grass". Ancient and modern pharmacological and clinical studies have proved that Ganoderma lucidum does have the effect of preventing and curing diseases, and prolonging life. The "Shen Nong's Materia Medica" in the Eastern Han Dynasty and the "Compendium of Materia Medica" by Li Shizhen, a famous medical scientist in the Ming Dynasty, all have detailed and extremely positive records on the efficacy of Ganoderma lucidum. As a traditional Chinese medicinal material with thousands of years of medicinal history, Ganoderma lucidum has high medicinal value. Modern p...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCY02A40/28
Inventor 田果廷赵永昌李树红柴红梅
Owner 云南省农业科学院生物技术与种质资源研究所
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