Selenium-rich pleurotus citrinopileatus cultivation fungus bag and manufacturing method thereof

Abstract

The invention discloses a selenium-rich pleurotus citrinopileatus cultivation fungus bag. The selenium-rich pleurotus citrinopileatus cultivation fungus bag is prepared from, by weight, 82%-84% of sawdust, 14%-16% of bran, 0.5%-1.5% of lime, 0.5%-1.5% of gypsum and 1 mg / kg-50 mg / kg of nano-selenium. A suitable microenvironment is provided for growth of hyphae through a stable base material structure, rapid planting of the hyphae is promoted, sufficient energy is provided for growth of the hyphae, and more nutrient substances are accumulated for development of sporocarps.

Description

Technical Field

[0001] The invention relates to the technical field of Pleurotus citrinopileatus cultivation, and in particular to a selenium-rich mushroom bag for Pleurotus citrinopileatus cultivation and a preparation method thereof. Background Art

[0002] In modern agricultural production, selenium-rich edible fungi have become an important focus for the development of functional agricultural products due to their combined nutritional benefits and health benefits. As a high-protein, low-fat edible and medicinal fungus, the nutritional and economic value of Pleurotus citriodora can be significantly enhanced through selenium-enriched cultivation.

[0003] At present, the cultivation of selenium-rich Pleurotus citrinopileatus usually adopts the method of directly adding nano-selenium or selenite into the culture medium. For example, in the prior art, nano-selenium is often added to base materials such as sawdust and bran at a dosage of 1 to 50 mg / kg, attempting to achieve selenium enrichment in the fruiting body through mycelium absorption and conversion.

[0004] However, this type of technology has exposed many technical defects in actual production: (1) This method directly adds selenium to the culture medium. Since selenium particles are prone to agglomeration in the base material, the loss rate of selenium during sterilization and cultivation is high; (2) Unbound selenium ions are easily free in aqueous solution, which on the one hand destroys the mycelial cell structure, and on the other hand, due to the influence of the pore structure of the base material, the distribution gradient of selenium in the mushroom stick is significantly different. Local high concentration of selenium inhibits mycelial growth, while low concentration areas cannot meet the enrichment requirements; (3) Traditional mushroom bag production relies on manual physical adjustment of tightness. Too loose can easily lead to matrix disintegration and contamination by foreign bacteria, while too tight can affect oxygen circulation, resulting in hypoxic growth of mycelium. The fundamental reason is that there is a lack of organic connection between the base material particles, making it difficult to form a stable structure. In summary, the mushroom bag production method and selenium addition method in the existing technology are no longer suitable for actual production. Summary of the Invention

[0005] The present invention aims to provide a selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus and a preparation method thereof, and fundamentally solves the defects of the prior art by introducing functional additives.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A selenium-rich mushroom bag for cultivating citronella mushroom comprises the following components in percentage by weight: 82-84% sawdust, 14-16% bran, 0.5-1.5% lime, 0.5-1.5% gypsum, 1-50 mg / kg nano-selenium, and 0.1-0.5% flocculant; the flocculant is one or more combinations of chitosan, sodium alginate, or polyacrylamide.

[0008] In some embodiments, the amount of nano-selenium added is 5-10 mg / kg, and the flocculant is chitosan, and the amount of chitosan added is 0.2-0.3%.

[0009] A method for preparing a selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus, comprising the following steps:

[0010] Preparation and mixing: Sawdust, bran, lime, and gypsum are weighed in proportion and mixed evenly; nano-selenium and flocculant are dissolved in water to prepare a mixed solution, which is evenly sprayed on the main ingredients, stirred until the water content reaches 60-65%, and the pH is adjusted to 6.0-7.0 to obtain a base material;

[0011] Base material processing: store the mixed base material for 12 to 24 hours to make it fully moistened; stick making: put the base material into the mushroom bag and make mushroom sticks with appropriate tightness;

[0012] Sterilization and inoculation: sterilize the mushroom sticks at 121±1℃ for 2.5-3.5 hours, and inoculate the cultivar of Pleurotus citrinopileatus on a clean bench after cooling.

[0013] Cultivation and fruiting: The inoculated mushroom sticks are cultured in an incubator at 22-24℃ until the mycelium bags are full, then transferred to the greenhouse, the temperature is controlled at 18-22℃, and the mushrooms are opened for fruiting.

[0014] In some embodiments, when preparing the mixed solution of the flocculant and nano-selenium, the temperature is controlled at 25-35° C., the stirring speed is 200-300 rpm, and the mixing time is 10-15 minutes.

[0015] In some embodiments, the sterilized mushroom sticks are cooled to 20-28° C. before inoculation, and the cooling time is 1.5-2.5 hours.

[0016] In some embodiments, during the mushroom production management stage, the relative humidity in the greenhouse is controlled at 80-90%, and ventilation is performed 2-3 times a day, each time for 15-30 minutes.

[0017] Working principle and beneficial effects of the present invention:

[0018] To address these issues, existing technologies have attempted improvements in two areas: first, optimizing the form of selenium addition, such as converting inorganic selenium into organic selenium. However, organic selenium is expensive to prepare and has poor stability, making it difficult to apply on a large scale. Second, adjusting the culture medium formula, such as increasing the proportion of bran or adding other colloidal substances, can alter the carbon-nitrogen ratio of the base, affecting the balance of mycelial growth. Furthermore, it's difficult to balance the water-holding capacity and air permeability of the colloidal substances. For example, some studies have attempted to improve the structure of the base by adding agar, but agar is easily degraded after high-temperature sterilization and cannot maintain long-term structural stability. It also hinders oxygen diffusion, leading to anaerobic respiration of the mycelium.

[0019] Flocculants (such as chitosan) contain a large number of polar groups such as amino and hydroxyl groups in their molecules. Under acidic conditions (culture medium pH around 6.5), they carry a positive charge and can form stable chelates with negatively charged nano-selenium particles through electrostatic adsorption. This chelation prevents the aggregation of nano-selenium particles, maintaining their nano-scale dispersion in the base material and increasing their contact area with the mycelium. Furthermore, the formed chelate slowly releases free selenium ions under the action of enzymes secreted by the mycelium (such as proteases and cellulases), ensuring a continuous supply of selenium.

[0020] Furthermore, flocculants, as natural high-molecular polymers, form a colloidal network after dissolving in aqueous solution. The flocculant molecular chains interweave between solid particles such as sawdust and bran, causing the loose base material particles to aggregate into a stable porous structure. This solves the problem of traditional mushroom sticks being "too loose and easy to fall apart, too tight and airtight." This ensures oxygen flow while maintaining sufficient water-holding capacity. During the fruiting stage, when water is frequently applied, the hydrophilic groups of the flocculants absorb water molecules to form a gel layer, inhibiting excessive water absorption and expansion of the base material particles and keeping the mushroom stick structure stable in a humid environment. This characteristic effectively solves the problem of pore clogging caused by water absorption in the base material used in traditional methods.

[0021] Flocculants fix selenium and improve its distribution, reducing the toxicity of high-concentration selenium to mycelium while increasing the effective selenium supply ( ); secondly, the stable substrate structure provides a suitable microenvironment for mycelial growth, promoting rapid mycelial colonization; finally, the improvement in nutrient retention and conversion efficiency not only provides sufficient energy for mycelial growth, but also accumulates more nutrients for fruiting body development ( , ultimately achieving a dual increase in yield and quality). BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a graph showing the effects of different formulas on the fruiting days and yield of Pleurotus citrinopileatus.

[0023] Figure 2 Schematic diagram showing that spraying nano-selenium can significantly improve the drought resistance of Pleurotus citrinopileatus. DETAILED DESCRIPTION

[0024] The following is further described in detail through specific implementation methods:

[0025] 1. Experimental Process

[0026] (1) Test materials

[0027] The mother culture of Pleurotus citriodora was provided by Guizhou Xinhui Agricultural Development Co., Ltd.; biological nano-selenium was purchased from Lvhuinong Biotechnology (Shenzhen) Co., Ltd., and the selenium content of the nano-selenium mother solution was 10 g / L.

[0028] (2) Test methods

[0029] 1. Preparation of Bag Materials for Pleurotus citrinopileatus Cultivation

[0030] The ingredients were prepared with 83% sawdust, 15% bran, 1% lime, and 1% gypsum. During the mixing process, different concentrations of bio-nanoselenium (0 mg / kg, 1 mg / kg, 5 mg / kg, 10 mg / kg, 20 mg / kg, 30 mg / kg, and 50 mg / kg) were added. The mixture was made into 300 g dry material and approximately 65% ​​moisture content. The mixture was then sterilized in a high-pressure steam autoclave at 121°C for 180 minutes. The specific recipe is shown in Table 1.

[0031] Table 1 Design formula of Pleurotus citrinopileatus cultivation medium

[0032]

[0033] 2. Inoculation and Cultivation

[0034] Place the sterilized cultivation bag in a super-bacterial workbench with the original cultivar of Pleurotus citrinopileatus. After inoculation, place it in a 23°C incubator with adequate ventilation and dark protection. During the mycelial growth stage, regularly mark the bag and record the mycelial growth (mycelial growth rate = mycelial growth length / growth days). Once the mycelium fills the bag, transfer it to the fruiting shed for cultivation. The humidity in the fruiting shed is 80%-90%, and ventilation is maintained.

[0035] 3. Index determination

[0036] Collect the fruiting bodies of Pleurotus citrinopileatus that meet the cap size range of 4cm-5cm, weigh and record the fresh weight after picking, place the sample in a 105℃ oven for 30 minutes, and then bake at 70℃ to constant weight, and record the dry weight. Use a mortar to grind the dried mushrooms and seal them for storage to measure the content of amino acids, total phenols, reducing sugars, soluble sugars, selenium and heavy metals. The determination of amino acids, total phenols, reducing sugars, and soluble sugars was carried out using the corresponding test kit operating requirements. The kit was purchased from Suzhou Mengxi Biotechnology Co., Ltd. The selenium and heavy metal contents were sent to Guizhou Mangshu Testing Technology Co., Ltd. for testing.

[0037] 2. Results Analysis

[0038] 2.1 Effects of nano-selenium on the mycelial growth of Pleurotus citrinopileatus

[0039] The mycelial growth and development results in Table 2 show that the addition of nano-selenium to the cultivation medium has a significant effect on the mycelial growth of P. citrinum. Among them, the mycelial growth rate was the fastest in Formulation A, which was significantly increased by 25.17% compared to the control. The time it took for Formulations A, B, and C to fill the bag with mycelium was significantly shorter than the control, shortening the bag-filling time by 16.51%, 13.04%, and 15.65%, respectively. Furthermore, the mycelium was white, dense, and grew well. This indicates that the addition of 1mg / kg to 10mg / kg of nano-selenium to the cultivation medium can promote the mycelial growth of P. citrinum, with the best effect when the nano-selenium concentration was 1mg / kg.

[0040] Table 2 Growth of mycelium in Pleurotus citrinopileatus cultivation

[0041]

[0042] Note: ++, +++, ++++ indicate that the mycelial density changes from sparse to dense.

[0043] 2.2 Effect of Nano-Selenium on the Yield of Pleurotus citrinopileatus

[0044] like Figure 1 As shown in the results, the effects of different formulations on the fruiting time and yield of Pleurotus citrinum were analyzed. The results showed that formulations A, B, and C produced the earliest fruiting period, with the earliest fruiting period being 32 days. The control formulation produced the latest fruiting period (49 days), significantly slower than the formulation with nano-selenium. Formulation C had the highest yield per crop, reaching 72.02g / stick, significantly higher than the control formulation (49.72g / stick). The yield of the nano-selenium-added formulations was significantly higher than that of the control formulation. These results indicate that adding 1-50mg / kg of nano-selenium can shorten the fruiting period and increase the yield of Pleurotus citrinum.

[0045] 4.3 Effects of Nano-Selenium on Selenium Content in Pleurotus citrinopileatus Fruiting Bodies

[0046] Compared to the control formulation, the addition of nano-selenium significantly increased the selenium content of Pleurotus citrinopileatus fruiting bodies, and the selenium content in the fruiting bodies of Pleurotus citrinopileatus was proportional to the amount of nano-selenium added (Table 3). According to the industry standard "Selenium-Enriched Agricultural Products" (GH / T 1135-2017), edible fungi are classified as selenium-enriched when their total selenium content is within the range of 0.10-5.00 mg / kg DW. In this study, the selenium content of the fruiting bodies of Pleurotus citrinopileatus cultivated with 1 mg / kg and 5 mg / kg nano-selenium formulations in the cultivation medium was 0.92 mg / kg DW and 4.93 mg / kg DW, respectively, meeting the standard requirements for selenium-enriched edible fungi.

[0047] Table 3 Changes in selenium content in Pleurotus citrinopileatus fruiting bodies of various recipes

[0048]

[0049]

[0050] Effects of 4.4 nano-selenium on heavy metal contents in the fruiting bodies of Pleurotus citrinopileatus

[0051] According to the "National Food Safety Standard for Limits of Contaminants in Food" (GB 2762), the main heavy metal indicators that need to be tested for edible fungi and their products include arsenic, mercury, lead, and cadmium. The "National Food Safety Standard for Limits of Contaminants in Food" (GB 2761-2017) clearly stipulates the limit values ​​for arsenic, mercury, lead, and cadmium in edible fungi. The limit values ​​for arsenic in edible fungi are 0.5 mg / kg, 0.1 mg / kg for mercury, 0.5 mg / kg for lead, and 0.2 mg / kg for cadmium. As shown in Table 4, the arsenic, mercury, lead, and cadmium levels in all formulations are generally below the limit values ​​for edible fungi specified in the national food safety standards. This indicates that under the experimental conditions, the effect of the addition of nano-selenium in different formulations on the heavy metal content of Pleurotus citrinopileatus fruiting bodies did not exceed the national limit standards. The heavy metal content of Pleurotus citrinopileatus treated with each formulation met the national food safety requirements.

[0052] Table 4 Changes in heavy metal content in Pleurotus citrinopileatus fruiting bodies of different recipes

[0053]

[0054] Effects of 4.5 nanometer selenium on nutritional quality of Pleurotus citrinopileatus fruiting bodies

[0055] Analysis of the effects of different formulations on the nutritional quality of Pleurotus citrinopileatus fruiting bodies revealed that the addition of nano-selenium to the cultivation medium significantly increased the amino acid content of Pleurotus citrinopileatus fruiting bodies. Formulation B had the highest amino acid content, with a 50.46% increase compared to the control formulation, indicating that the addition of nano-selenium promoted amino acid synthesis or accumulation in Pleurotus citrinopileatus. Reducing sugar content did not differ significantly between the different formulations compared to the control formulation, indicating that nano-selenium had a minimal effect on reducing sugar content in Pleurotus citrinopileatus. The addition of nano-selenium to the cultivation medium significantly decreased the soluble sugar content in Pleurotus citrinopileatus fruiting bodies, indicating that nano-selenium can reduce the accumulation of soluble sugars in Pleurotus citrinopileatus fruiting bodies. Formulations B and C had the highest total phenolic content, significantly increasing by 21.18% and 28.43%, respectively, compared to the control formulation. Formulations D and F had no significant differences in total phenolic content compared to the control formulation.

[0056] Table 5 Changes in nutrient content of Pleurotus citrinopileatus fruiting bodies in various recipes

[0057]

[0058] Effects of 4.6 nano-selenium on drought resistance of Pleurotus citrinopileatus

[0059] In a relatively dry environment, the fruiting bodies of the control formula of Pleurotus citrinopileatus are prone to dead mushrooms, but the fruiting bodies of the formula with added nano-selenium grow significantly better than the control formula. Therefore, it is speculated that nano-selenium may improve the drought resistance of Pleurotus citrinopileatus. In order to explore the effect of nano-selenium on the drought resistance of Pleurotus citrinopileatus, young Pleurotus citrinopileatus fruiting bodies with similar morphology in different parts of the same mushroom stick were selected, or young Pleurotus citrinopileatus fruiting bodies with consistent growth on different mushroom sticks were selected. The group sprayed with aqueous solution was used as the control group, and the group sprayed with the same amount of nano-selenium solution with different concentrations was used as the treatment group. The concentrations of nano-selenium were set to 25μg / L, 100μg / L, 500μg / L, 1mg / L, 5mg / L, and 10mg / L, respectively. The spraying treatment was performed 3 times a day. After 2 days of treatment, the spraying treatment was stopped and drought stress treatment was performed. The growth of Pleurotus citrinopileatus was observed, and it was found that the growth of Pleurotus citrinopileatus sprayed with 500μg / L-5mg / L nano-selenium was better than that of the water treatment. Figure 2 As shown in the results, when spraying 5 mg / L nano-selenium with only aqueous solution under simulated drought conditions, the citrus mushrooms that were sprayed with the nano-selenium solution died in the fruiting body stage, while the citrus mushrooms that were sprayed with the nano-selenium solution did not die under the simulated drought conditions, indicating that nano-selenium can improve the drought resistance of citrus mushrooms.

[0060] In summary, the use of bionanoselenium as a selenium enrichment agent in Pleurotus citrinopileatus cultivation demonstrates its enhanced bioavailability and safety. Compared to traditional selenium sources, bionanoselenium has a smaller particle size and larger specific surface area, making it more readily absorbed and converted by Pleurotus citrinopileatus mycelium. Leveraging the nano-effects and bioactivity of bionanoselenium, efficient selenium absorption and conversion by Pleurotus citrinopileatus is achieved, making Pleurotus citrinopileatus a highly effective organic selenium-enriched food. This innovative application opens new avenues for selenium enrichment in edible fungi, expands the application of nanotechnology in agricultural biology, and provides new ideas and methods for the selenium-enriched cultivation of other edible fungi varieties. The topic and research approach are both innovative and practical, not only possessing academic significance but also promoting the application of scientific research results.

[0061] 2. To verify the effect of flocculants in bacterial bags

[0062] (1) Grouping Table 6 is as follows:

[0063] Table 6 - Grouping table

[0064]

[0065] The following data in Table 7 were obtained:

[0066] Table 7 - Results

[0067]

[0068] The following conclusions were drawn:

[0069] 1. Comparative analysis of full bag time

[0070] The CK group (blank control) had a bag-filling time of 38 days, which was still the longest among all groups, indicating that the mycelium growth efficiency was the lowest when there was no nano-selenium and flocculant, and the insufficient stability of the base material structure restricted the colonization and spread of mycelium.

[0071] The T1 group (5 mg / kg nano-selenium, no chitosan) took 33 days to fill the bag, which was 5 days shorter than the CK group. This shows that 5 mg / kg nano-selenium has a significant promoting effect on mycelial growth, but due to the lack of structural support from chitosan, the effect is not as good as the experimental group with added flocculants.

[0072] The E1 group (5 mg / kg nano-selenium + 0.2% chitosan) took 30 days to fill the bag, which was 3 days shorter than the T1 group and the shortest among all groups. This shows that when chitosan and 5 mg / kg nano-selenium work synergistically, they can significantly accelerate the process of mycelium filling the bag by optimizing the base material structure and improving the distribution of selenium.

[0073] The bag-filling time of E2 group (5 mg / kg nano-selenium + 0.3% chitosan) was 31 days, slightly longer than that of E1 group. This may be because the high dose of chitosan slightly increased the viscosity of the base material and slightly decreased the local air permeability, resulting in slightly slower mycelial growth.

[0074] 2. Comparative analysis of mycelial growth rate

[0075] The growth rate of group CK was 0.58 cm / d, the lowest among all groups, reflecting that the loose substrate and uneven nutrient distribution restricted mycelial expansion under natural conditions.

[0076] The growth rate of the T1 group was 0.65 cm / d, which was 12.1% higher than that of the CK group, indicating that 5 mg / kg nano-selenium can promote mycelial metabolism, but the promotion effect is limited due to the possible local aggregation of selenium particles.

[0077] The growth rate of group E1 was 0.76 cm / d, which was 16.9% higher than that of group T1 and the highest among all groups. This confirmed that chitosan optimized the pore structure of the base material by forming a colloidal network, and at the same time formed a stable chelate with nano-selenium to reduce toxicity, providing a more suitable growth microenvironment (oxygen and water balance) for mycelium.

[0078] The growth rate of group E2 was 0.73 cm / d, slightly lower than that of group E1, which further indicated that at a dosage of 0.3% chitosan, the slight decrease in the air permeability of the substrate had a certain inhibitory effect on the mycelial growth rate.

[0079] 3. Comparative analysis of mushroom yield in one crop

[0080] The yield of the CK group was 49.72 g / stick, which was a basic level. The lack of selenium fortification and structural support resulted in low nutrient conversion efficiency.

[0081] The yield of the T1 group was 65.30 g / stick, which was 31.3% higher than that of the CK group, indicating that 5 mg / kg nano-selenium can promote fruiting body development through nutritional fortification, but the yield increase was limited due to the insufficient stability of the base material structure.

[0082] The yield of group E1 was 82.10 g / rod, which was 25.7% higher than that of group T1. This indicates that the addition of chitosan under the condition of 5 mg / kg nano-selenium can not only reduce the problem of loose or tight base material by stabilizing the structure, but also improve the effective utilization rate of selenium and significantly increase the fruiting body yield.

[0083] The yield of group E2 was 79.80 g / stick, slightly lower than that of group E1, suggesting that high-dose chitosan may affect the air permeability of the base material due to its excessive water retention, resulting in slightly restricted fruiting body development.

[0084] 4. Comparative analysis of selenium content in dry weight of mushroom caps

[0085] The selenium content in the CK group was 0.06 mg / kg, indicating that selenium enrichment in the fruiting bodies was low when there was no selenium source.

[0086] The selenium content of group T1 was 4.52 mg / kg, which met the standard of selenium-rich edible fungi (0.1-5.00 mg / kg), indicating that 5 mg / kg of nano-selenium could achieve selenium enrichment in the fruiting body, but due to the lack of the fixation effect of chitosan, the selenium absorption efficiency was limited.

[0087] The selenium content in the E1 group was 6.35 mg / kg, which was 40.5% higher than that in the T1 group, indicating that the chelation effect of chitosan could reduce the aggregation and loss of 5 mg / kg nano-selenium and improve the absorption and conversion efficiency of selenium by mycelium.

[0088] The selenium content in group E2 was 6.88 mg / kg, slightly higher than that in group E1, indicating that increasing the chitosan dosage can further enhance the fixation of low-dose nano-selenium and improve the selenium enrichment effect.

[0089] In summary, when the addition amount of nano-selenium is 5 mg / kg, chitosan can still significantly improve the growth efficiency, yield and selenium enrichment of Pleurotus citriodora through the synergistic effect of optimizing the base material structure and promoting selenium absorption. Among them, the 0.2% chitosan ratio has the best comprehensive effect, and the selenium content of the fruiting body meets the selenium-rich standard, which is both safe and economical.

Claims

1. A selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus, characterized in that: The invention comprises the following components in percentage by weight: 82-84% of sawdust, 14-16% of bran, 0.5-1.5% of lime, 0.5-1.5% of gypsum and 1-50mg / kg of nano-selenium.

2. The selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus according to claim 1, characterized in that The added amount of nano-selenium is 5-10 mg / kg.

3. The selenium-enriched mushroom bag for cultivating Pleurotus citrinopileatus according to claim 2 further comprises 0.1-0.5% of a flocculant, wherein the flocculant is one or more combinations of chitosan, sodium alginate or polyacrylamide.

4. The method for preparing a selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus according to any one of claims 1 to 3, characterized in that: The steps include: Preparation and mixing: Sawdust, bran, lime, and gypsum are weighed in proportion and mixed evenly; nano-selenium and flocculant are dissolved in water to prepare a mixed solution, which is evenly sprayed on the main ingredients, stirred until the water content reaches 60-65%, and the pH is adjusted to 6.0-7.0 to obtain a base material; Base material treatment: Store the mixed base material for 12 to 24 hours to allow the base material to be fully moistened; Making sticks: Put the base material into the mushroom bag and make mushroom sticks with appropriate tightness; Sterilization and inoculation: sterilize the mushroom sticks at 121±1℃ for 2.5-3.5 hours, and inoculate the cultivar of Pleurotus citrinopileatus on a clean bench after cooling. Cultivation and fruiting: The inoculated mushroom sticks are cultured in an incubator at 22-24℃ until the mycelium bags are full, then transferred to the greenhouse, the temperature is controlled at 18-22℃, and the mushrooms are opened for fruiting.

5. The selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus according to claim 4, characterized in that: When preparing the mixed solution of the flocculant and nano-selenium, the temperature is controlled at 25-35° C., the stirring speed is 200-300 rpm, and the mixing time is 10-15 minutes.

6. The selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus according to claim 5, characterized in that: The sterilized mushroom sticks are cooled to 20-28° C. before inoculation, and the cooling time is 1.5-2.5 hours.

7. The selenium-rich mushroom bag for cultivating Pleurotus citrinopileatus according to claim 6, characterized in that: During the mushroom production management stage, the relative humidity in the greenhouse is controlled at 80-90%, and ventilation is performed 2-3 times a day, each time for 15-30 minutes.

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