New biological material for preventing and controlling plants from plant diseases and insect pests

A technology of pests and diseases and new materials, applied in pest control, plant growth regulators, plant growth regulators, etc., can solve the problems of reduced activity of polymers and effects affected by molecular weight

Inactive Publication Date: 2017-07-25
成都特普生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These reports also pointed out that the effect of polysaccharides is greatly affected by molecular weight. When the degree of polymerization of monosaccharides exceeds 3, the activity of the polymer decreases significantly with the increase of degree of polymerization and molecular weight.

Method used

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  • New biological material for preventing and controlling plants from plant diseases and insect pests
  • New biological material for preventing and controlling plants from plant diseases and insect pests

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0018] The combination was prepared with 25 g of soluble starch, 5 g of calcium carbonate, 2 g of potassium dihydrogen phosphate, and 5 g of ammonium sulfate, plus 4.0 mL of corn oil, with a natural pH, and 1000 mL of water to make a liquid medium, and sterilized at 121°C for 20 min. Use an inoculation loop to transfer the purified conidia of Trichoderma harzianum to a test tube containing sterile water, stir to disperse the conidia from the mycelium, filter the mycelium with sterile gauze, and then measure the concentration of the spores. Adjust to 10 7 cfu / mL, according to the inoculum size of 1%, inoculated into the liquid fermentation medium, cultured at 28°C and 150r / min for 48 hours, at this time, the pH of the culture solution dropped to 2.1, and 10g of sterilized chitosan, 5g of lecithin and 5g of sodium hydrogen phosphate, continue to cultivate for 72 hours, filter and collect the reaction solution, and obtain aminoaminopolyglycolate with a polymerization degree of 50...

Embodiment approach 2

[0019] Embodiment 2 The effect of amino amino polysaccharide ester on preventing and treating plant virus disease

[0020] The aminoamino polysaccharide ester prepared in Embodiment 1 is used for controlling capsicum. The capsicum with virus disease has occurred was selected for the test, and the aminoamino polyglycoside was divided into three concentration dilutions as treatments, 50 plants for each treatment, and repeated 4 times. The test was set as 5 treatments: treatment 1: aminoaminoglycan ester diluted 100 times of medicinal solution; treatment 2: aminoaminoglycan ester diluted 300 times of medicinal solution; treatment 3: aminoaminoglycan ester diluted 500 times of medicinal solution; Treatment 4: virus nemesis; treatment 5: water control. Spray once every 5 days, and investigate the incidence rate after using it three times. The results are shown in the table, the control plants were seriously ill, the chemical pesticide virus killer could partially inhibit the viru...

Embodiment approach 3

[0024] Embodiment 3 The effect of amino amino polysaccharide ester on controlling aphids

[0025] The aminoaminoglycan ester prepared in Embodiment 1 was used for the aphid control test. Leek was used as the test crop. Potted leeks were planted in greenhouses, and the number of insects per plant was investigated. Amino polyglycosides were diluted in three concentrations as the treatment. The chemical pesticide imidacloprid was treated and water was used as the control. 10 pots per treatment, 5 plants per pot, repeated 4 times . The test was set as 5 treatments in total: treatment 1: 500 times of dilution of the aminoaminoglycan ester; treatment 2: 300 times of dilution of the aminoaminoglycan ester; treatment 3: 100 times of dilution of the aminoglycan ester; Treatment 4: imidacloprid was diluted 500 times; treatment 5: water control. Investigate the number of insect populations 24 hours after spraying. The results are shown in Table 2. The clean water control plants had a...

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Abstract

The invention relates to a new biological material for preventing and controlling plants from plant diseases and insect pests, particularly to a preparation method of a biological pesticide using a new biological material amino polysaccharide ester as a main component, and belongs to the technical field of biological pesticides and biochemistry. According to the present invention, the new biological material with advantages of efficient prevention and control of plant diseases and insect pests of crops and crop growth promoting is prepared through the biological reaction action of microorganisms or biological enzymes, and the positive significance is provided for the reduction of the dependence of agricultural production on chemical pesticides.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to new biological materials used for preventing and controlling plant diseases and insect pests and promoting plant growth. Background technique [0002] With the requirements of economic development and sustainable environmental development, many countries have introduced policy measures to reduce the use of chemical pesticides and fertilizers. Finding new agricultural disease control measures that conform to the sustainable development of agriculture has become a hot spot in current agricultural production research. Due to the advantages of no pollution and no residue, biological pesticide products have attracted people's attention. With the development of the chemical industry and the expansion of the use of pesticides and fertilizers, the number and variety of chemical pesticides are increasing. Now the world's total output of chemical pesticides (in terms of active ingredients) exce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/16A01P1/00A01P3/00A01P5/00A01P7/04A01P21/00C08B37/00C12P19/26C12R1/885
CPCA01N57/16C08B37/00C12P7/6481C12P19/26Y02A50/30
Inventor 黄永
Owner 成都特普生物科技股份有限公司
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