A kind of miniature Chinese rose induction method
A technique for inducing rose and flower buds, applied in the field of cell engineering, can solve the problems of reduced ornamental quality, difference in in vitro flowering rate, affecting flowering, etc., and achieves the effects of increased ornamental value, bright color and petite body.
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[0051] The present invention will be further described below in conjunction with implementation case:
[0052] In the in vitro rapid propagation method of a miniature rose stem section described above, the culture medium adopted in each step is prepared by conventional methods with distilled water. in:
[0053] Bud induction and proliferation medium:
[0054] YJ1: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 70g / L;
[0055] YJ2: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 65g / L;
[0056] YJ3: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 55g / L;
[0057] YJ4: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 40g / L;
[0058] YJ5: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 30g / L;
[0059] YJ6: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 15g / L.
[0060] Flower bud induction culture medium:
[0061] MS+TDZ1.0mg / L+NAA0.1mg / L+activated carbon 0.6g / L+sucrose 30g / L+agar 7g / L.
[0062] 1. The composition of the culture medium
[0063] (1) The composition of MS medium is: KNO 3 1900mg·L -1 , NH 4 NO 3 1650mg·L -1 , CaCl 2 2H 2 O440mg·L -...
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