A kind of miniature Chinese rose induction method

A technique for inducing rose and flower buds, applied in the field of cell engineering, can solve the problems of reduced ornamental quality, difference in in vitro flowering rate, affecting flowering, etc., and achieves the effects of increased ornamental value, bright color and petite body.

Inactive Publication Date: 2016-01-20
JILIN NORMAL UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

A large number of test results show that for negative plants, shading can speed up their growth and improve their ornamental quality; while positive plants need sufficient light for effective photosynthesis, so that the plants have more flowers and better flower quality. In general, shading is not conducive to the formation of anthocyanins, and the content of anthocyanins is significantly reduced, resulting in lighter flower colors and lower ornamental quality. The type of photoperiod affects the test-tube flowering of many plants
Some plants have large differences in tube flowering rate between short-day and long-day treatments

Method used

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  • A kind of miniature Chinese rose induction method
  • A kind of miniature Chinese rose induction method
  • A kind of miniature Chinese rose induction method

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Embodiment 1

[0051] The present invention will be further described below in conjunction with implementation case:

[0052] In the in vitro rapid propagation method of a miniature rose stem section described above, the culture medium adopted in each step is prepared by conventional methods with distilled water. in:

[0053] Bud induction and proliferation medium:

[0054] YJ1: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 70g / L;

[0055] YJ2: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 65g / L;

[0056] YJ3: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 55g / L;

[0057] YJ4: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 40g / L;

[0058] YJ5: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 30g / L;

[0059] YJ6: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 15g / L.

[0060] Flower bud induction culture medium:

[0061] MS+TDZ1.0mg / L+NAA0.1mg / L+activated carbon 0.6g / L+sucrose 30g / L+agar 7g / L.

[0062] 1. The composition of the culture medium

[0063] (1) The composition of MS medium is: KNO 3 1900mg·L -1 , NH 4 NO 3 1650mg·L -1 , CaCl 2 2H 2 O440mg·L -...

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Abstract

The invention belongs to the field of cell engineering technology, relates to a minisize China rose induction method, and discloses an efficient rapid minisize China rose bud induction method including obtaining of sterile seedlings, China rose bud induction culture, flower culture and other steps. According to the method, pot cultured minisize China rose is used as a plant material, in a stem segment in vitro regeneration system, a most suitable medium for minisize China rose blossoming induction is finally determined as MS + TDZ1.0mg / L + saccharose 40g / L + agar 7.2g / L, test-tube plantlet blossoming is successfully induced, the blossoming rate is increased to 87.1%, a minisize China rose test-tube plantlet little in posture and beautiful and bright in color is successfully cultivated, and the method has important theoretical and practical significance to the China rose asexual breeding.

Description

technical field [0001] The invention belongs to the technical field of cell engineering, and relates to a method for inducing miniature rose flowers, in particular to a method for inducing flowering of miniature rose plantlets in test tubes. Background technique [0002] Rosachinesis Minima belongs to the woody plant of Rosaceae Rosaceae. It is a new species of rose family. "Diamond Rose" is a popular miniature flower germplasm resource, with broad application market prospects and huge potential. At present, countries with large production and sales of potted flowers such as the United States, Denmark, and Canada have in-depth and systematic research on the growth and development of miniature roses, quality control of potted flowers, flowering regulation, product packaging, and post-harvest storage and transportation. Today, the United States and Japan have entered the stage of micro rose factory seedling cultivation, while China has just started. [0003] Flower is the ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周晓馥徐洪伟武慧
Owner JILIN NORMAL UNIV
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