Minisize China rose induction method
A technique for inducing rose flowers and flower buds, applied in the field of cell engineering, which can solve the problems of reduced ornamental quality, difference in flowering rate in test tubes, and impact on flowering, and achieve the effects of increased ornamental value, bright color, and petite body
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[0051] The present invention will be further explained below in conjunction with implementation cases:
[0052] In the above-mentioned method for in vitro rapid propagation of miniature rose stems, the medium used in each step is prepared with distilled water according to a conventional method. among them:
[0053] Bud induction and propagation medium:
[0054] YJ1: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 70g / L;
[0055] YJ2: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 65g / L;
[0056] YJ3: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 55g / L;
[0057] YJ4: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 40g / L;
[0058] YJ5: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 30g / L;
[0059] YJ6: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 15g / L.
[0060] Flower bud induction culture medium:
[0061] MS+TDZ1.0mg / L+NAA0.1mg / L+activated charcoal 0.6g / L+sucrose 30g / L+agar 7g / L.
[0062] 1. The composition of the culture medium
[0063] (1) MS medium composition: KNO 3 1900mg·L -1 , NH 4 NO 3 1650mg·L -1 , CaCl 2 ·2H 2 O440mg·L -1 , MgSO 4 ·7H 2 O370mg·L -1 , KH 2 PO...
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