Minisize China rose induction method

A technique for inducing rose flowers and flower buds, applied in the field of cell engineering, which can solve the problems of reduced ornamental quality, difference in flowering rate in test tubes, and impact on flowering, and achieve the effects of increased ornamental value, bright color, and petite body

Inactive Publication Date: 2014-10-29
JILIN NORMAL UNIV
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Problems solved by technology

A large number of test results show that for negative plants, shading can speed up their growth and improve their ornamental quality; while positive plants need sufficient light for effective photosynthesis, so that the plants have more flowers and better flower quality. In general, shading is not conducive to the f

Method used

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  • Minisize China rose induction method
  • Minisize China rose induction method
  • Minisize China rose induction method

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Embodiment 1

[0051] The present invention will be further explained below in conjunction with implementation cases:

[0052] In the above-mentioned method for in vitro rapid propagation of miniature rose stems, the medium used in each step is prepared with distilled water according to a conventional method. among them:

[0053] Bud induction and propagation medium:

[0054] YJ1: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 70g / L;

[0055] YJ2: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 65g / L;

[0056] YJ3: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 55g / L;

[0057] YJ4: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 40g / L;

[0058] YJ5: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 30g / L;

[0059] YJ6: MS+TDZ1.0mg / L+agar 7.2g / L+sucrose 15g / L.

[0060] Flower bud induction culture medium:

[0061] MS+TDZ1.0mg / L+NAA0.1mg / L+activated charcoal 0.6g / L+sucrose 30g / L+agar 7g / L.

[0062] 1. The composition of the culture medium

[0063] (1) MS medium composition: KNO 3 1900mg·L -1 , NH 4 NO 3 1650mg·L -1 , CaCl 2 ·2H 2 O440mg·L -1 , MgSO 4 ·7H 2 O370mg·L -1 , KH 2 PO...

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Abstract

The invention belongs to the field of cell engineering technology, relates to a minisize China rose induction method, and discloses an efficient rapid minisize China rose bud induction method including obtaining of sterile seedlings, China rose bud induction culture, flower culture and other steps. According to the method, pot cultured minisize China rose is used as a plant material, in a stem segment in vitro regeneration system, a most suitable medium for minisize China rose blossoming induction is finally determined as MS + TDZ1.0mg / L + saccharose 40g / L + agar 7.2g / L, test-tube plantlet blossoming is successfully induced, the blossoming rate is increased to 87.1%, a minisize China rose test-tube plantlet little in posture and beautiful and bright in color is successfully cultivated, and the method has important theoretical and practical significance to the China rose asexual breeding.

Description

Technical field [0001] The invention belongs to the technical field of cell engineering and relates to a method for inducing miniature rose flowers, in particular to a method for inducing the flowering of miniature rose test tube seedlings. Background technique [0002] Rosa chinesis Minima (Rosa chinesis Minima) is a woody plant in the Rosaceae Rosa genus. It is a new species of the rose family. It has a short plant type, dense flowering, rich flower color, slightly scented flowers, and long flowering period. It is also called because of its unique character. "Diamond Rose" is a currently popular micro-flower germplasm resource with broad application market prospects and huge potential. At present, countries with large pot flower production and sales, such as the United States, Denmark, and Canada, have conducted in-depth and systematic research on the growth and development of miniature roses, pot flower quality control, flowering period regulation, product packaging and post-h...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周晓馥徐洪伟武慧
Owner JILIN NORMAL UNIV
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