Water culture seedling hardening method and device of beautiful millettia root tissue culture seedlings
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A technology of Niu Dali and tissue culture seedlings, which is applied in the direction of fertilization device, botany equipment and method, cultivation, etc. It can solve the problems affecting the growth of Niu Dali seedlings and the root damage of tissue culture seedlings, so as to reduce the breeding of bacteria and improve the quality of transplanting. The survival rate and the effect of dark green leaves
Inactive Publication Date: 2020-10-13
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Due to the different characteristics of the tissue culture seedlings, attention should be paid to the configuration of the nutrient solution and the method of hydroponic control in the hydroponics of the tissue culture seedlings, so as to avoid damage to the roots of the tissue culture seedlings caused by unreasonable nutrient solution configuration and affect the growth of Niu Dali seedlings
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Embodiment 1
[0047] A method for hydroponic seedling hardening of bovine vigorously tissue-cultured seedlings, using the method attached to the present invention figure 1 The hydroponic seedling hardening device comprises the steps:
[0048] Step 1, preparation before transplanting: clean and disinfect the cultivation tank containing the nutrient solution in the hydroponic tank, put the nutrient solution into the cultivation tank, and the nutrient solution per 10L contains the following components: Ca(NO 3 ) 2 .4H 2 O 720mg, KNO 3 430mg, KH 2 PO 4 110mg, MgSO 4 .7H 2 O 380mg, Na 2 Fe-EDTA 21mg, H 3 BO 3 1.5mg, CuSO 4 .5H 2 O 0.3mg, MnSO 4 .4H 2 O0.2mg, ZnSO 4 .7H 2 O 0.4mg, (NH 4 ) 2 MoO 4 0.1mg, paclobutrazol 300mg, triacontanol 0.5mg, sodium nitrophenolate 8mg, brassinolide 0.01mg and nucleotide 5mg; wherein, the pH value of the nutrient solution is 5.8-6.5, and the reagent for adjusting the pH is quality The fraction is 5% nitric acid solution and the mass fraction...
Embodiment 2
[0052] A method for hydroponic seedling hardening of bovine vigorously tissue-cultured seedlings, using the method attached to the present invention figure 1 The hydroponic seedling hardening device comprises the steps:
[0053] Step 1, preparation before transplanting: clean and disinfect the cultivation tank containing the nutrient solution in the hydroponic tank, put the nutrient solution into the cultivation tank, and the nutrient solution per 10L contains the following components: Ca(NO 3 ) 2 .4H 2 O 750mg, KNO 3 480mg, KH 2 PO 4 130mg, MgSO 4 .7H 2 O 410mg, Na 2 Fe-EDTA 23mg, H 3 BO 3 1.9mg, CuSO 4 .5H 2 O 0.6mg, MnSO 4 .4H 2 O0.5mg, ZnSO 4 .7H 2 O 0.8mg, (NH 4 ) 2 MoO 4 0.3mg, paclobutrazol 400mg, triacontanol 1mg, sodium nitrophenolate 14mg, brassinolide 0.05mg and nucleotide 10mg; wherein, the pH value of the nutrient solution is 5.8-6.5, and the reagent for adjusting the pH is the mass fraction 5% nitric acid solution and 5% sodium hydroxide solu...
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Abstract
The invention discloses a water culture seedling hardening method of beautiful millettia root tissue culture seedlings. The method comprises the following steps of 1) making preparation before transplanting: disinfecting a seedling hardening environment and preparing a nutrient solution with the pH value of 5.8-6.5, wherein every 10 L of the nutrient solution contains 0.01-0.05 mg of brassinolide,5-10 mg of nucleotide and other nutritional ingredients; 2) planting the water culture seedlings: planting the disinfected beautiful millettia root seedlings on a planting plate, placing the plantingplate on a cultivation tank filled with the nutrient solution, immersing one part of the root system into the nutrient solution, and not immersing the other part of the root system into the nutrientsolution; and 3) performing water culture management: controlling the temperature, humidity and illumination intensity of the seedling hardening environment according to regulations, replacing the nutrient solution when not more than 1/3 of the root system is immersed in the nutrient solution, and hardening the seedlings for a period of time. According to the method, the promotion effect of the brassinolide and the nucleotide is utilized, the moisture and nutrients of the root system are sufficient, and a part of roots are remained to be in contact with air for breathing, so that the root system is facilitated to grow and the transplanting survival rate is increased.
Description
technical field [0001] The invention relates to the technical field of seedling hardening of tissue cultured seedlings. More specifically, the present invention relates to a hydroponic seedling hardening method and a seedling hardening device of bovine vigorously tissue-cultured seedlings. Background technique [0002] Tissue culture seedling hardening, seedling hardening is domestication, the purpose is to improve the adaptability of tissue culture seedlings to external environmental conditions, improve their photosynthetic ability, promote the robustness of tissue culture seedlings, and finally achieve the purpose of improving the survival rate of tissue culture seedlings transplanting . Generally, domestication requires adjustment of temperature, humidity, light, and the presence of sterile environmental elements. Within a few days of domestication, the environmental conditions should be similar to those at the time of cultivation; in the later stages of domestication, t...
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