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Tissue culture quick-breeding seedling raising method for radix asparagi

A technology for rapid breeding of seedlings and asparagus groups, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem that each plant can only get 3-5 plants, and there are few reports on the rooting rate. Low reproductive germination rate and other problems, to achieve the effect of short cultivation period, high rooting rate, and reduce browning

Active Publication Date: 2017-02-01
GUANGXI MODERN GARDEN GREENING ENGINEERING SEEDLING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the germination rate of seed propagation is low and the emergence time is long; the reproduction coefficient of division propagation is low, and each plant can only be divided into 3-5 plants
In recent years, a new propagation method, that is, tissue culture propagation method, has emerged for the propagation of Asparagus, but there are few reports on the rooting rate of this method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of Asparagus tissue culture rapid propagation seedling raising method, concrete steps are as follows:

[0036] (1) Sterilization: cut the twigs of Asparagus Radix as explants, rinse them with tap water, then cut them into 1.0-2.0cm long small stem segments, put them into 1% detergent aqueous solution and soak them for 5min, then Rinse with tap water for 20 minutes; disinfect with 75% alcohol on the ultra-clean workbench for 10 seconds, then rinse with sterile water; then soak in 1g / L mercuric chloride for 5 minutes, and then rinse with sterile water for 5 minutes until clean; after taking it out, blot the water dry with sterile filter paper, and cut off the necrotic part with a knife;

[0037] (2) Inoculation and callus induction: the sterilized explants were inoculated on the induction medium formulated as 1 / 2MS+1.0mg / L6-BA+0.5mg / L NAA, and each bottle was inoculated with 3 -5 branches, after standing for 5 days, a small amount of callus germinated at most of t...

Embodiment 2

[0042] A kind of Asparagus tissue culture rapid propagation seedling raising method, concrete steps are as follows:

[0043] (1) Sterilization: cut the twigs of Asparagus Radix as explants, rinse them with tap water, then cut them into 1.0-2.0cm long small stem segments, put them into 1% detergent solution and soak them for 8 minutes, then Rinse with tap water for 20 minutes; disinfect with 75% alcohol on the ultra-clean workbench for 40 seconds, then rinse with sterile water; then soak in 1g / L mercuric chloride for 10 minutes, and then rinse with sterile water for 5 minutes until clean; after taking it out, blot the water dry with sterile filter paper, and cut off the necrotic part with a knife;

[0044] (2) Inoculation and callus induction: the sterilized explants were inoculated on the induction medium formulated as 1 / 2MS+1.0mg / L6-BA+0.5mg / L NAA, and each bottle was inoculated with 3 -5 branches, after standing for 8 days, a small amount of callus germinated at most of the...

Embodiment 3

[0049] A kind of Asparagus tissue culture rapid propagation seedling raising method, concrete steps are as follows:

[0050] (1) Sterilization: cut the twigs of Asparagus Radix as explants, rinse them with tap water, then cut them into 1.0-2.0cm long small stem segments, put them into 1% detergent solution and soak them for 8 minutes, then Rinse with tap water for 20 minutes; disinfect with 75% alcohol on the ultra-clean workbench for 20 seconds, then rinse with sterile water; then soak in 1g / L mercuric chloride for 8 minutes, and then rinse with sterile water for 5 minutes until clean; after taking it out, blot the water dry with sterile filter paper, and cut off the necrotic part with a knife;

[0051] (2) Inoculation and callus induction: the sterilized explants were inoculated on the induction medium formulated as 1 / 2MS+1.0mg / L6-BA+0.5mg / L NAA, and each bottle was inoculated with 3 -5 branches, after standing for 4-6 days, a small amount of callus germinated at most of th...

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Abstract

The invention discloses a tissue culture quick-breeding seedling raising method for radix asparagi and belongs to the technical field of biology. The method includes the steps of: 1) sterilization; 2) inoculation and callus induction; 3) callus differentiation and adventitious bud induction; 4) seedling reinforcing and rooting cultivation; and 5) domestication and transplanting. A novel rooting culture medium is employed, wherein harmful substances and bacteria in the novel rooting culture medium are eliminated and explant browning is relieved. The rooting culture medium is rich in various beneficial minerals, and has effects of inducing and promoting synthesis of abscisic acid and cytokinin, so that the method can change hormonal equilibrium in the radix asparagi, effectively regulates growth and development of the radix asparagi and promotes and accelerates rooting of the radix asparagi. The method saves time cost and has high rooting rate being more than 95%.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of biotechnology, in particular to a method for rapid propagation of asparagus seedlings through tissue culture. [0003] 【Background technique】 [0004] Asparagus is a lily plant. Also known as asparagus. Cold in nature, sweet in taste, slightly bitter. It has the effects of nourishing yin and clearing away heat, nourishing the lungs and nourishing the kidneys. It is used to treat fever due to yin deficiency, cough and hematemesis, lung abscess, sore throat, thirst, constipation and other diseases. Asparagus root contains 33% starch, 4% sucrose and other nutrients. Asparagus whole herb contains asparagine (asparagine), β-sitosterol, solid saponins, mucilage, furfural derivatives, 17 kinds of amino acids, rich vitamins, inorganic elements, stigmasterol, lactones, flavonoids , anthraquinone and cardiac glycosides and other components. [0005] Asparagus is a precious traditional Chinese medicine resource,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
CPCA01H4/001A01H4/008
Inventor 黄东香
Owner GUANGXI MODERN GARDEN GREENING ENGINEERING SEEDLING CO LTD
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