Anti-hair loss oil-controlling and antibacterial composition, shampoo and conditioner

Abstract

The present invention belongs to the field of shampoo and hair care products, and provides an anti-hair loss, oil-control and antibacterial composition, a shampoo and a conditioner. The anti-hair loss, oil-control and antibacterial composition provided by the present invention is composed of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract in a mass ratio of 0.5:0.5:3:2:1:1. Based on the composition, the present invention also provides an anti-hair loss, oil-control and antibacterial shampoo and a conditioner. The components of the anti-hair loss, oil-control and antibacterial composition of the present invention have good effects in inhibiting hair loss, inhibiting sebum secretion, inhibiting bacteria, etc., and there is a synergistic effect between the components in inhibiting sebum secretion and inhibiting bacteria, which can improve the comprehensive performance of existing shampoo and hair care products.

Description

Technical Field

[0001] The invention belongs to the field of hair washing and hair care products, and relates to an anti-hair loss oil-controlling and antibacterial composition, a shampoo and a hair conditioner. Background Art

[0002] As people's pace of life continues to accelerate and the pressure of life and work continues to increase, hair loss has become one of the common problems faced by the social population. According to statistics from the National Health Commission, more than 250 million people in my country suffered from hair loss in 2019. Among various hair loss problems, the incidence of seborrheic alopecia is relatively high. Seborrheic alopecia is caused by excessive secretion of sebum on the scalp, which gradually shrinks and damages the hair follicles, resulting in hair loss. At the same time, due to excessive secretion of sebum on the scalp, problems such as greasy scalp, dandruff, and itchy scalp often occur.

[0003] In order to solve these problems, people have developed different compositions.For example, CN201911188348.7 discloses a kind of anti-hair loss shampoo and its preparation method, the raw materials of the anti-hair loss shampoo include ginger extract, sophora flavescens root extract, cypress leaf extract, ligusticum chuanxiong extract, sodium laureth sulfate, cocamide MEA, ethylene glycol distearate, cetearyl alcohol, cocamidopropyl betaine, guar hydroxypropyl trimethyl ammonium chloride, polyquaternium-10, sodium hyaluronate, citric acid, zinc pyrithione, dimethicone, pyridoxine, carbomer, panthenol, phenoxyethanol, sodium benzoate, EDTA-disodium, CI16255, CI19140, CI42090, essence, and the remainder is water.The preparation method of the shampoo is simple, non-irritating and sensitizing to the body, and its anti-hair loss and antipruritic properties are strong at the same time. For another example, CN201710487537.9 discloses an anti-hair loss shampoo, comprising the following substances in parts by weight: 100 parts of water, 10-15 parts of amino acid foaming agent, 1-3 parts of vitamin E, 25-35 parts of sesame stem soaking liquid, 35-45 parts of walnut soaking liquid, 72-80 parts of plant surfactants, 25-35 parts of coconut betaine, 2-4 parts of hyaluronic acid, and 2-3 parts of collagen. The shampoo can provide acid and nutrients to the scalp, reduce dandruff, promote hair growth, prevent hair loss, and effectively maintain the health of the scalp and hair. Although the above-mentioned products have achieved certain effects in preventing hair loss, they cannot effectively inhibit sebum secretion and effectively inhibit bacteria, and their comprehensive performance is still unsatisfactory. Therefore, in order to better meet people's demand for shampoo products with better comprehensive performance such as anti-hair loss and oil control, it is very necessary to develop compositions with functions such as anti-hair loss, oil control, and antibacterial, as well as related shampoo and hair care products. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an anti-hair loss oil-controlling and antibacterial composition, a shampoo and a conditioner, so as to utilize the synergistic effect between the components in the composition to effectively inhibit skin oil secretion and effectively inhibit bacteria, thereby improving the comprehensive performance of existing shampoo and hair care products.

[0005] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention is as follows:

[0006] A hair loss prevention, oil control and antibacterial composition, the composition is composed of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract, the Platycladus orientalis leaf extract is obtained by continuous circulation extraction of dried Platycladus orientalis leaves with an ethanol aqueous solution, the Forsythia suspensa extract is obtained by continuous circulation extraction of dried Forsythia suspensa with an ethanol aqueous solution, the Sophora flavescens extract is obtained by extracting dried Sophora flavescens with a supercritical CO2 extraction method, and the Artemisia argyi extract is obtained by extracting dried Artemisia argyi with a supercritical CO2 extraction method; in the composition, the mass ratio of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract is 0.5:0.5:3:2:1:1.

[0007] In the technical scheme of the above-mentioned anti-hair loss, oil-control and antibacterial composition, the preparation method of the Platycladus orientalis leaf extract is: using an ethanol aqueous solution with a volume percentage of 70% to 75% of ethanol as an extractant, continuously cyclically extracting the dried Platycladus orientalis leaf powder under normal pressure, controlling the weight ratio of the Platycladus orientalis leaf powder to the extractant to be 1:(5 to 10), and the extraction time to be 2 to 4 hours, and distilling and concentrating the obtained extract until the volume fraction of ethanol is less than 5%.

[0008] In the technical scheme of the above-mentioned anti-hair loss, oil-control and antibacterial composition, the preparation method of the Forsythia suspensa extract is: using an ethanol aqueous solution with a volume percentage of 90% to 95% of ethanol as an extractant, continuously cyclically extracting the dried Forsythia suspensa powder under normal pressure, controlling the weight ratio of Forsythia suspensa powder to the extractant to be 1:(3 to 5), and the extraction time to be 2 to 4 hours, and distilling and concentrating the obtained extract until the volume fraction of ethanol is less than 5%.

[0009] In the technical scheme of the above-mentioned anti-hair loss, oil-control and antibacterial composition, the preparation method of the Sophora flavescens extract is: placing the dried Sophora flavescens powder in an extraction kettle, and extracting for 4 to 6 hours under the conditions of an extraction temperature of 40°C, an extraction pressure of 32MPa, and a CO2 flow rate of 20 to 25kg / h.

[0010] In the technical scheme of the above-mentioned anti-hair loss, oil-control and antibacterial composition, the preparation method of the mugwort extract is: placing dry mugwort powder in an extraction kettle, and extracting for 4 to 6 hours under the conditions of an extraction temperature of 45°C, an extraction pressure of 32MPa, and a CO2 flow rate of 20 to 25kg / h.

[0011] In the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial composition, the molecular weight of the hydrolyzed keratin is ≥100,000 Daltons. The hydrolyzed keratin can be purchased from the market or prepared by itself with reference to the prior art. A feasible method for preparing the hydrolyzed keratin is as follows:

[0012] The animal hair is cleaned with water, and the cleaned animal hair is placed in a sodium mercaptoacetate solution with a pH value of 8 to 12 and a concentration of 0.3 to 1.0 mol / L (the pH value is adjusted with a NaOH solution), and soaked at normal pressure and room temperature for 1 to 3 hours, and then urea is added to make the concentration of urea in the solution reach 6.0 to 8.0 mol / L, and the reaction is carried out at normal pressure and 20 to 60° C. for 3 to 28 hours, and the undissolved raw materials are filtered out, and the hydrolyzate solution is obtained by centrifugation; the obtained hydrolyzate solution is loaded into a dialysis bag with a relative molecular weight cutoff of 100,000 Daltons (100 kD), and dialyzed in a mercaptoethanol aqueous solution with a mass concentration of 0.08% for at least 40 hours, and freeze-dried to obtain hydrolyzed keratin with a molecular weight of ≥100,000 Daltons.

[0013] In the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial composition, the type XVII collagen can be purchased on the market.

[0014] Based on the above-mentioned anti-hair loss, oil control and antibacterial composition, the present invention provides an anti-hair loss, oil control and antibacterial shampoo, wherein the mass fraction of the above-mentioned anti-hair loss, oil control and antibacterial composition in the shampoo is 0.5% to 10%.

[0015] Furthermore, in the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial shampoo, the shampoo is preferably composed of the following components in percentage by mass:

[0016]

[0017] In the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial shampoo, according to the needs of actual application, the shampoo may also contain at least one of a fragrance and a preservative, the mass percentage of the fragrance in the shampoo is 0.5% to 1%, and the mass percentage of the preservative in the shampoo is 0.05% to 0.1%.

[0018] In the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial shampoo, the water may be deionized water or distilled water.

[0019] In the technical scheme of the above-mentioned anti-hair loss, oil-control and antibacterial shampoo, the surfactant is at least two of ammonium laureth sulfate, ammonium lauryl sulfate, sodium laureth sulfate, sodium laureth carboxylate, sodium lauroyl sarcosinate, potassium lauroyl glycinate, sodium lauryl sulfate and sodium laureth sulfosuccinate; the softening conditioning agent is polydimethylsiloxane, polydimethylsiloxane alcohol, amino-terminated polydimethylsiloxane, aminobispropyl polydimethylsiloxane, cyclopentasiloxane, polyquaternium-10, polyquaternium-7, polyquaternium-47, PEG-7 glyceryl cocoate, guar hydroxypropyltrimonium chloride, polyethylene glycol-90M, At least two of polyethylene glycol-14M and polyethylene glycol-7M; the thickener is at least two of coconut acid alkylamide, cocamide DEA, cocamide MEA, cocamide methyl MEA, cocamide MIPA, cocamidopropyl betaine, lauryl betaine, lauryl hydroxysulfobetaine, lauryl glucoside, laurin glyceride, lauryl lactate, sodium lauroamphoacetate, cetearyl alcohol, cetyl alcohol, xanthan gum, carbomer, polyacrylic acid, hydroxyethyl cellulose, and hydroxypropyl methylcellulose; the acid-base regulator is at least one of citric acid, sodium citrate, lactic acid, sodium lactate, ethylenediaminetetraacetic acid, and sodium salt of ethylenediaminetetraacetic acid.

[0020] The preparation method of the above-mentioned shampoo with oil control and antibacterial function is as follows: add a surfactant, a softening conditioner, an acid-base regulator, and a thickener to part of the water in sequence, heat to 60-80°C at normal pressure under stirring, and stop heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cool the obtained mixed solution to 40-45°C, add the anti-hair loss oil control and antibacterial composition, fragrance (if such component), and preservative (if such component) in sequence, and then add the remaining water, continue stirring until the components are fully mixed, and cool to below 40°C to obtain the mixture.

[0021] Based on the anti-hair loss, oil-controlling and antibacterial composition, the present invention also provides an anti-hair loss, oil-controlling and antibacterial conditioner, which contains the anti-hair loss, oil-controlling and antibacterial composition, and the mass fraction of the anti-hair loss, oil-controlling and antibacterial composition in the conditioner is 0.3% to 5%.

[0022] Furthermore, in the technical solution of the above-mentioned anti-hair loss, oil-controlling and antibacterial hair conditioner, the hair conditioner is preferably composed of the following components in percentage by mass:

[0023]

[0024] In the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial conditioner, according to the needs of actual application, the conditioner may also contain at least one of a fragrance and a preservative, the mass percentage of the fragrance in the conditioner is 0.5% to 1%, and the mass percentage of the preservative in the conditioner is 0.05% to 0.1%.

[0025] In the technical solution of the above-mentioned anti-hair loss, oil-control and antibacterial conditioner, the water can be deionized water or distilled water.

[0026] In the technical scheme of the above-mentioned anti-hair loss oil-controlling and antibacterial conditioner, the hair conditioning agent is behenyl trimethyl ammonium chloride, behenyl trimethyl ammonium sulfate, stearyl trimethyl ammonium chloride, cetrimonium chloride, polydimethylsiloxane, cyclopentasiloxane, polydimethylsiloxane alcohol, aminopropyl polydimethylsiloxane, amino-terminated polydimethylsiloxane, polyquaternium-10, polyquaternium-7, guar hydroxypropyl trimethyl ammonium chloride, polyethylene glycol-14M, polyethylene glycol-7 The thickener is at least one of cetyl alcohol, cetearyl alcohol, behenyl alcohol, ceteareth-25, glyceryl stearate, polyquaternium-37, ceteareth-20, xanthan gum, acrylic acid polymer, hydroxyethyl cellulose, and hydroxypropyl methylcellulose; the acid-base regulator is at least one of citric acid, sodium citrate, lactic acid, sodium lactate, ethylenediaminetetraacetic acid, and sodium salt of ethylenediaminetetraacetic acid.

[0027] The preparation method of the above-mentioned anti-hair loss, oil-control and antibacterial conditioner is as follows: add a hair conditioner, an acid-base regulator and a thickener into water, heat to 60-80°C at normal pressure under stirring, stop heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cool the obtained mixed solution to 40-45°C, add the anti-hair loss, oil-control and antibacterial composition, essence (if such component) and preservative (if such component) in sequence, continue stirring until each component is fully mixed, and cool to below 40°C to obtain the product.

[0028] The components of the anti-hair loss, oil-control and antibacterial composition provided by the present invention have good effects in inhibiting hair loss, inhibiting sebum secretion, and inhibiting bacteria. At the same time, there is a synergistic effect between the components in inhibiting sebum secretion and inhibiting bacteria. This is mainly due to the reasonable matching of the types and proportions of the components in the composition, which improves the scalp microenvironment, regulates the self-renewal of scalp hair loss-related cells, promotes the replacement of scalp epidermis, and stabilizes hair follicles; the improvement of the scalp microenvironment can inhibit excessive secretion of sebaceous glands, prevent sebum accumulation in hair follicles, keep the nutrient channels of hair follicles unobstructed, and improve the development and growth of hair follicles; the improvement of the scalp microenvironment can also inhibit bacterial growth, especially it can strengthen the inhibition of Staphylococcus aureus and Propionibacterium acnes that aggravate seborrheic alopecia, which can better maintain scalp health and reduce hair loss. The details are as follows:

[0029] (1) The present invention has been verified through experiments on reducing hair loss: compared with before using the anti-hair loss oil-controlling and antibacterial shampoo of the present invention, after using the anti-hair loss oil-controlling and antibacterial shampoo of the present invention for 8 weeks, the average number of hair loss of volunteers during shampooing has been reduced by more than half; compared with before using the anti-hair loss oil-controlling and antibacterial composition of the present invention, after using the anti-hair loss oil-controlling and antibacterial composition of the present invention for 7 weeks, the average number of hair loss of volunteers during the process of combing their hair 50 times after shampooing has been reduced by more than half. This shows that long-term use of the anti-hair loss oil-controlling and antibacterial composition or shampoo of the present invention can effectively reduce hair loss during shampooing and combing after shampooing.

[0030] (2) The present invention has confirmed through the tensile force test required to pull out and break hair: compared with before using the anti-hair loss oil-control antibacterial shampoo of the invention, after using the anti-hair loss oil-control antibacterial shampoo of the invention for 10 weeks, the tensile force required to pull out the hair of volunteers has increased effectively, and the increase has reached 39%; compared with before using the anti-hair loss oil-control antibacterial shampoo of the invention, after using the anti-hair loss oil-control antibacterial shampoo of the invention for 10 weeks, the tensile force required to break the hair of volunteers has increased effectively, and the increase has reached 32.6%. This shows that long-term use of the anti-hair loss oil-control antibacterial shampoo of the invention can enhance the vitality of hair follicles, effectively stabilize hair follicles and enhance hair toughness, and reduce hair loss.

[0031] (3) The present invention has confirmed through a sebum secretion inhibition experiment that hydrolyzed keratin, type XVII collagen and mugwort extract with a molecular weight ≥100,000 Daltons do not have the effect of inhibiting skin cortical secretion. Although Forsythia suspensa extract, Sophora flavescens extract and Platycladus orientalis leaf extract all have a certain effect of inhibiting skin sebum secretion, the inhibitory effect is limited. After the present invention forms an anti-hair loss, oil control and antibacterial composition comprising components including type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and mugwort extract in a specific proportion, the inhibitory effect of the composition on skin sebum secretion is significantly improved, which indicates that the components of the anti-hair loss, oil control and antibacterial composition described in the present invention have a synergistic effect on inhibiting sebum secretion.

[0032] (4) The present invention has been confirmed by antibacterial experiments: compared with the single use of Forsythia suspensa extract, Sophora flavescens extract, Platycladus orientalis leaf extract, and Artemisia argyi extract, the anti-hair loss oil control antibacterial composition of the present invention has significantly better antibacterial performance against Escherichia coli, Staphylococcus aureus, Clostridium tetani, Propionibacterium acnes, Pseudomonas aeruginosa, Candida albicans, Salmonella and Staphylococcus albus, which indicates that the present invention forms an anti-hair loss oil control antibacterial composition according to a specific ratio of components including type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract, and the components of the composition have a synergistic effect on antibacterial performance. At the same time, the anti-hair loss oil control antibacterial composition of the present invention can effectively inhibit the growth of Malassezia, which is the main factor causing dandruff and scalp itching, which indicates that the anti-hair loss oil control antibacterial composition of the present invention can alleviate the problems of dandruff and scalp itching by effectively inhibiting the growth of Malassezia.

[0033] Compared with the prior art, the technical solution provided by the present invention produces the following beneficial technical effects:

[0034] 1. The present invention provides an anti-hair loss oil control and antibacterial composition, which is composed of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract in a mass ratio of 0.5:0.5:3:2:1:1. Experiments show that the components in the composition have a synergistic effect in inhibiting skin cortical secretion and antibacterial effects. The composition can not only effectively inhibit skin oil secretion, but also has a good inhibitory effect on common bacteria and fungi. At the same time, long-term use of the anti-hair loss oil control and antibacterial composition of the present invention can effectively reduce hair loss during shampooing and combing after shampooing, and can also enhance the vitality of hair follicles, effectively stabilize hair follicles and enhance hair toughness, thereby reducing hair loss. The composition provided by the present invention has good effects of stabilizing hair follicles, enhancing hair toughness, preventing hair loss, controlling oil and inhibiting bacteria, and can solve the problem that existing shampoo and hair care products have certain anti-hair loss performance but are poor in inhibiting sebum secretion and inhibiting bacteria, and the comprehensive performance is not satisfactory, and can better meet people's demand for shampoo products with better comprehensive performance such as anti-hair loss, oil control and antibacterial performance.

[0035] 2. Based on the above-mentioned anti-hair loss, oil control and antibacterial composition, the present invention also provides shampoo and conditioner containing the anti-hair loss, oil control and antibacterial composition, which also have the functions of preventing hair loss, inhibiting cortical secretion and inhibiting, enriching the forms of shampoo and hair care products with anti-hair loss, oil control and antibacterial properties, and can better meet the different application needs of consumers in actual application scenarios. DETAILED DESCRIPTION

[0036] The anti-hair loss oil-controlling and antibacterial composition, shampoo and conditioner provided by the present invention are further described by way of examples below. It is necessary to point out that the following examples are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. The technical personnel in the relevant field make some non-essential improvements and adjustments to the present invention according to the above invention content for specific implementation, which still falls within the scope of protection of the present invention.

[0037] In the following examples, except for Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract, other components were purchased from the market. In the mixed solution of methylchloroisothiazolinone and methylisothiazolinone prepared in a mass ratio of 3:1, the total mass concentration of methylchloroisothiazolinone and methylisothiazolinone was 1.5%.

[0038] The following is an anti-hair loss, oil control and antibacterial composition prepared by Example 1, an anti-hair loss, oil control and antibacterial shampoo prepared by Examples 2 to 10, an anti-hair loss, oil control and antibacterial conditioner prepared by Examples 11 to 19, and performance tests performed by Examples 20 to 26.

[0039] Example 1

[0040] In this embodiment, the anti-hair loss, oil control and antibacterial composition is prepared in the following steps:

[0041] (1) Preparation of Platycladus orientalis leaf extract by continuous cycle extraction method

[0042] The naturally dried Platycladus orientalis leaves are crushed to obtain Platycladus orientalis leaf powder, and an ethanol aqueous solution with a volume percentage of 70% is used as an extractant. The Platycladus orientalis leaf powder is continuously cyclically extracted under normal pressure, the weight ratio of the Platycladus orientalis leaf powder to the extractant is controlled to be 1:7, and the extraction time is 3 hours. The obtained extract is distilled and concentrated to the volume fraction of ethanol less than 5%, thereby obtaining the Platycladus orientalis leaf extract.

[0043] (2) Preparation of Forsythia suspensa extract by continuous cycle extraction method

[0044] The naturally dried Forsythia suspensa was crushed to obtain Forsythia suspensa powder, and an ethanol aqueous solution with a volume percentage of 95% was used as an extractant. The Forsythia suspensa powder was subjected to continuous cycle extraction under normal pressure, the weight ratio of the Forsythia suspensa powder to the extractant was controlled to be 1:4, and the extraction time was 3 hours. The obtained extract was distilled and concentrated until the volume fraction of ethanol was less than 5%, thereby obtaining a Forsythia suspensa extract.

[0045] (3) Preparation of Sophora flavescens extract by supercritical CO2 extraction

[0046] The naturally dried Sophora flavescens was crushed to obtain Sophora flavescens powder, and the Sophora flavescens powder was placed in a supercritical CO2 extraction kettle, and extracted for 5 hours under the conditions of extraction temperature of 40°C, extraction pressure of 32MPa, and CO2 flow rate of 25kg / h to obtain Sophora flavescens extract.

[0047] (4) Preparation of Artemisia argyi extract by supercritical CO2 extraction

[0048] The naturally dried mugwort leaves are crushed to obtain mugwort leaf powder, and the mugwort leaf powder is placed in a supercritical CO2 extraction kettle, and extracted for 4.5 hours under the conditions of extraction temperature of 45°C, extraction pressure of 32MPa, and CO2 flow rate of 23kg / h to obtain mugwort leaf extract.

[0049] (5) Preparation of anti-hair loss oil control and antibacterial composition

[0050] The hydrolyzed keratin with a molecular weight of ≥100,000 Dalton, type XVII collagen, the Platycladus orientalis leaf extract prepared in step (1), the Forsythia suspensa extract prepared in step (2), the Sophora flavescens extract prepared in step (3), and the Artemisia argyi extract prepared in step (4) are measured in a mass ratio of 0.5:0.5:3:1:2:1, and the measured components are fully mixed to obtain an anti-hair loss, oil-control, and antibacterial composition.

[0051] Example 2

[0052] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0053]

[0054] Add a surfactant, a softening conditioner, an acid-base regulator, and a thickener to a reactor filled with 10 kg of deionized water in sequence, heat to 60-80° C. at normal pressure under stirring, and stop heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cool the obtained mixed solution to 40° C., add the anti-hair loss, oil-control, and antibacterial composition prepared in Example 1, and then add 71.5 kg of deionized water, continue stirring for at least 10 minutes to fully mix the components, and obtain an anti-hair loss, oil-control, and antibacterial shampoo.

[0055] Example 3

[0056] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0057]

[0058] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener to a reactor filled with 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition and essence prepared in Example 1, and then adding 70.1 kg of deionized water, and continuing stirring for at least 10 minutes to fully and evenly mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0059] Example 4

[0060] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0061]

[0062] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener to a reactor containing 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 45° C., adding the composition with oil control and antibacterial function prepared in Example 1, and then adding 71.5 kg of deionized water, continuing stirring for at least 10 minutes to fully mix the components, and cooling the mixture to below 40° C. to obtain the shampoo with oil control and antibacterial function.

[0063] Add a surfactant, a softening conditioner, an acid-base regulator, and a thickener to a reactor filled with 10 kg of deionized water in sequence, heat to 60-80° C. at normal pressure under stirring, and stop heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cool the obtained mixed solution to 40° C., add the anti-hair loss, oil-control, and antibacterial composition prepared in Example 1, and then add 70.3 kg of deionized water, continue stirring for at least 10 minutes to fully mix the components, and obtain an anti-hair loss, oil-control, and antibacterial shampoo.

[0064] Example 5

[0065] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0066]

[0067] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener into a reactor filled with 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1, a flavor and a preservative, and then adding 68.05 kg of deionized water, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0068] Example 6

[0069] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0070]

[0071] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener into a reactor filled with 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1, and then adding 67.5 kg of deionized water, and continuing stirring for at least 10 minutes to fully and evenly mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0072] Example 7

[0073] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0074]

[0075] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener to a reactor containing 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1 and a preservative, and then adding 66.05 kg of deionized water, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0076] Example 8

[0077] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0078]

[0079] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener into a reactor filled with 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1, and then adding 65.3 kg of deionized water, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0080] Example 9

[0081] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0082]

[0083]

[0084] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener into a reactor filled with 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1, and then adding 64.5 kg of deionized water, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0085] Example 10

[0086] In this embodiment, an anti-hair loss, oil-control and antibacterial shampoo is prepared. The components of the shampoo and the content of each component are as follows:

[0087]

[0088] The preparation method of the shampoo comprises the following steps: adding a surfactant, a softening conditioner, an acid-base regulator and a thickener into a reactor filled with 10 kg of deionized water in sequence, heating the mixture to 60-80° C. at normal pressure under stirring, and stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution; cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1, and then adding 63.9 kg of deionized water, and continuing stirring for at least 10 minutes to fully and evenly mix the components to obtain the anti-hair loss, oil-control and antibacterial shampoo.

[0089] Embodiment 11

[0090] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0091]

[0092] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 83.6 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-controlling and antibacterial composition prepared in Example 1, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-controlling and antibacterial hair conditioner.

[0093] Example 12

[0094] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0095]

[0096] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 82.75 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition and essence prepared in Example 1, continuing stirring for at least 10 minutes to fully mix the components, and obtaining the anti-hair loss, oil-control and antibacterial hair conditioner.

[0097] Embodiment 13

[0098] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0099]

[0100] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 84.0 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-controlling and antibacterial composition prepared in Example 1, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-controlling and antibacterial hair conditioner.

[0101] Embodiment 14

[0102] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0103]

[0104]

[0105] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 83.6 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1, a fragrance and a preservative, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-control and antibacterial hair conditioner.

[0106] Embodiment 15

[0107] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0108]

[0109] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 85.7 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-controlling and antibacterial composition prepared in Example 1, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-controlling and antibacterial hair conditioner.

[0110] Example 16

[0111] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0112]

[0113] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 85.75 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-control and antibacterial composition prepared in Example 1 and a preservative, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-control and antibacterial hair conditioner.

[0114] Embodiment 17

[0115] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0116]

[0117] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 86.1 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-controlling and antibacterial composition prepared in Example 1, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-controlling and antibacterial hair conditioner.

[0118] Embodiment 18

[0119] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0120]

[0121] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioner, an acid-base regulator and a thickener into a reactor filled with 86.55 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-controlling and antibacterial composition prepared in Example 1, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-controlling and antibacterial hair conditioner.

[0122] Embodiment 19

[0123] In this embodiment, an anti-hair loss, oil-control and antibacterial hair conditioner is prepared. The components of the hair conditioner and the content of each component are as follows:

[0124]

[0125]

[0126] The preparation method of the hair conditioner comprises the following steps: adding a hair conditioning agent, an acid-base regulator and a thickener into a reactor filled with 86.5 kg of deionized water, heating the mixture to 60-80° C. at normal pressure under stirring, stopping heating after each component is dissolved or evenly dispersed to obtain a mixed solution, cooling the obtained mixed solution to 40° C., adding the anti-hair loss, oil-controlling and antibacterial composition prepared in Example 1, and continuing stirring for at least 10 minutes to fully mix the components to obtain the anti-hair loss, oil-controlling and antibacterial hair conditioner.

[0127] Embodiment 20

[0128] In this embodiment, the performance of the anti-hair loss, oil control and antibacterial shampoo of the present invention in preventing hair loss during shampooing is tested, and the steps are as follows:

[0129] 1. Sample Preparation

[0130] The anti-hair loss, oil-controlling and antibacterial shampoo in Example 6 was used as the sample shampoo.

[0131] 2. Determination of volunteers and use of sample shampoo

[0132] 20 volunteers were selected, 10 males and 10 females, aged between 30 and 60 years old. Each volunteer washed their hair with the sample shampoo, using 10g of sample shampoo and massaging the scalp for 3 minutes each time, washing their hair 5 times a week for 12 consecutive weeks, and testing the number of hairs that fell during washing every Monday.

[0133] 3. Test results

[0134] The average number of hairs lost by each volunteer when washing their hair every Monday was counted, and the results are shown in Table 1. In Table 1, Week 0 represents the average number of hairs lost by the volunteer when washing their hair on the Monday before the volunteer used the sample shampoo for the first time.

[0135] Table 1 Test results of average hair loss of each volunteer during shampooing

[0136]

[0137] It can be seen from Table 1 that compared with before using the anti-hair loss, oil control and antibacterial shampoo of the invention, after continuously using the anti-hair loss, oil control and antibacterial shampoo of the invention for 8 weeks, the average number of hair loss of volunteers during shampooing has been reduced by more than half, which shows that long-term use of the anti-hair loss, oil control and antibacterial shampoo of the invention can effectively reduce hair loss during shampooing.

[0138] Embodiment 21

[0139] In this example, the performance of the anti-hair loss, oil control and antibacterial composition of the present invention in preventing hair loss after shampooing is tested, and the steps are as follows:

[0140] 1. Sample Preparation

[0141] The anti-hair loss, oil-control and antibacterial composition prepared in Example 1 was prepared into a solution with a composition mass concentration of 5% with deionized water to obtain sample A.

[0142] According to the formula in Example 8, the anti-hair loss, oil control and antibacterial composition in the formula was removed, that is, the shampoo was prepared according to the formula in Example 8 without adding the anti-hair loss, oil control and antibacterial composition to obtain sample B.

[0143] 2. Determination of volunteers and use of sample shampoo

[0144] 20 volunteers were selected, 10 males and 10 females, aged between 30 and 60 years old. When washing hair, drop sample A evenly on the scalp and massage for 5 minutes, then use 10g of sample B to wash hair (massage scalp for 3 minutes), wash hair 5 times a week for 10 consecutive weeks, and comb hair 50 times with a comb after washing hair and drying hair every Monday, and count the number of hairs dropped during the combing process.

[0145] 3. Test results

[0146] The average number of hairs that fell out of each volunteer after shampooing and combing their hair 50 times after the hair was dried was counted every Monday. The results are shown in Table 2. In Table 2, the results are shown in Table 1. In Table 2, Week 0 represents the average number of hairs that fell out of the volunteer after shampooing and combing their hair 50 times after the hair was dried on the Monday before the first use of Sample A + Sample B to wash their hair.

[0147] Table 2 Test results of average hair loss of each volunteer after shampooing

[0148]

[0149] It can be seen from Table 2 that compared with before using the anti-hair loss, oil control and antibacterial composition of the present invention, after continuously using the anti-hair loss, oil control and antibacterial composition of the present invention for 7 weeks, the average number of hair loss in the volunteers during the process of combing their hair 50 times after shampooing was reduced by more than half, which shows that long-term use of the anti-hair loss, oil control and antibacterial composition of the present invention can effectively reduce hair loss after shampooing.

[0150] Embodiment 22

[0151] In this embodiment, the effect of the anti-hair loss, oil-controlling and antibacterial shampoo of the present invention on the pulling force required for pulling out hair follicles is tested, and the steps are as follows:

[0152] 1. Sample Preparation

[0153] The anti-hair loss, oil-controlling and antibacterial shampoo in Example 6 was used as the sample shampoo.

[0154] 2. Determination of volunteers and use of sample shampoo

[0155] Five long-haired female volunteers aged between 30 and 40 were selected. Each volunteer used the sample shampoo to wash their hair, using 10 g of the sample shampoo and massaging the scalp for 3 minutes each time, washing their hair 5 times a week for 10 consecutive weeks.

[0156] 3. Test instruments and test methods

[0157] The testing instrument is a simple electronic tensile tester. Before the volunteers used the anti-hair loss oil-control and antibacterial shampoo of the present invention for the first time, and after using the anti-hair loss oil-control and antibacterial shampoo of the present invention for 5 weeks and 10 weeks, 3 hairs were randomly selected from the occipital / forehead / temples area, and the selected hairs were tied to the pull ring of the tensile tester near the hair roots. The tensile tester was pulled by hand, and the tensile force required for each hair to be pulled out was tested and recorded, and the data were statistically averaged. The results are shown in Table 3.

[0158] Table 3 Average pulling force required to pull out hair

[0159] Time of use Before use After 5 weeks of use After 10 weeks of use Tension(N) 0.87 1.09 1.21

[0160] It can be seen from Table 3 that compared with before using the anti-hair loss, oil control and antibacterial shampoo of the invention, the pulling force required to pull out the volunteers' hair increased significantly after continuous use of the anti-hair loss, oil control and antibacterial shampoo of the invention for 5 weeks. After continuous use of the anti-hair loss, oil control and antibacterial shampoo of the invention for 10 weeks, the pulling force required to pull out the volunteers' hair further increased, and the increase reached 39%. This shows that long-term use of the anti-hair loss, oil control and antibacterial shampoo of the invention can enhance the vitality of hair follicles, effectively stabilize hair follicles, and reduce hair loss.

[0161] Embodiment 23

[0162] In this embodiment, the effect of the anti-hair loss, oil-controlling and antibacterial shampoo of the present invention on the tensile force required for hair tensile testing is tested, and the steps are as follows:

[0163] 1. Sample Preparation

[0164] The anti-hair loss, oil-controlling and antibacterial shampoo in Example 5 was used as the sample shampoo.

[0165] 2. Determination of volunteers and use of sample shampoo

[0166] Ten volunteers were selected, 5 males and 5 females, aged between 30 and 50 years old. Each volunteer washed his hair with the sample shampoo, using 10g of the sample shampoo and massaging the scalp for 3 minutes each time, washing his hair 5 times a week for 10 consecutive weeks.

[0167] 3. Test instruments and test methods

[0168] The testing instrument is a universal tensile tester (model HY10080). Before the volunteers used the anti-hair loss oil-controlling and antibacterial shampoo of the present invention for the first time, and after using the anti-hair loss oil-controlling and antibacterial shampoo of the present invention for 5 weeks and 10 weeks, 3 hairs were randomly selected from the occipital / forehead / temples area for each person, and the selected hairs were pulled out, and the 3 cm of the pulled out hairs close to the hair roots were placed in the test range of the tensile tester for testing, and the tensile force required to break each hair was recorded, and the data were statistically averaged. The results are shown in Table 4.

[0169] Table 4 Average pulling force required to break hair

[0170] Time of use Before use After 5 weeks of use After 10 weeks of use Tension(N) 0.95 1.13 1.26

[0171] It can be seen from Table 4 that compared with before using the anti-hair loss, oil control and antibacterial shampoo of the invention, the pulling force required to break the volunteers' hair increased significantly after continuous use of the anti-hair loss, oil control and antibacterial shampoo of the invention for 5 weeks. After continuous use of the anti-hair loss, oil control and antibacterial shampoo of the invention for 10 weeks, the pulling force required to break the volunteers' hair further increased, and the increase reached 32.6%. This shows that long-term use of the anti-hair loss, oil control and antibacterial shampoo of the invention can enhance hair toughness.

[0172] Embodiment 24

[0173] In this embodiment, the performance of the anti-hair loss oil control and antibacterial composition in inhibiting sebum secretion is tested in the following steps:

[0174] 1. Sample Preparation

[0175] Set up 6 groups of samples, A, B, C, D, E, and F, and the samples in each group are as follows:

[0176] In group A, the anti-hair loss, oil-control and antibacterial composition prepared in Example 1 was prepared into a solution with a composition mass concentration of 5.0% using deionized water.

[0177] Group B: hydrolyzed keratin with a molecular weight of ≥100,000 Dalton and type XVII collagen were mixed in a mass ratio of 1:1 to form a composition, and the composition was prepared into a solution with a mass concentration of 2.0% with deionized water.

[0178] In group C, the Forsythia suspensa extract was prepared into a solution with a mass concentration of 3.0% using deionized water.

[0179] In group D, the Sophora flavescens extract was prepared into a solution with a mass concentration of 3.0% using deionized water.

[0180] In group E, the Platycladus orientalis leaf extract was prepared into a solution with a mass concentration of 5.0% using deionized water.

[0181] In group F, the wormwood leaf extract was prepared into a solution with a mass concentration of 3.0% using deionized water.

[0182] 2. Determination of volunteers and use of samples

[0183] 24 volunteers were selected, 12 males and 12 females, aged between 20 and 49 years old. The volunteers were divided into 6 groups, with 2 males and 2 females in each group. Each group of volunteers washed their faces with one of the samples in groups A, B, C, D, E, and F, once a day for 10 consecutive days. The operation of washing the face each time was: 5g of the sample was evenly applied to the face and pressed for 3 minutes, and then rinsed with tap water for 1 minute.

[0184] 3. Test Methods

[0185] Using the sebum tape method, let the volunteers sit and rest for 20 minutes, then wipe the forehead of the volunteers clean with 15% alcohol, and then stick the sebum tape (Cu Derm, USA) flatly and tightly to the skin surface of the volunteer's forehead. After 20 minutes, peel off the sebum tape and put it flat on a clean glass slide to get the sebum sample. Put the sebum sample into a scanner (ScanMaker V310), and analyze the scanning results using image analysis software Adobe Photoshop5.5 and Image-Pro PlusV1.2.

[0186] The sebum secretion content in the forehead of each group of volunteers was measured by the sebum tape method when the volunteers did not use the samples and 1 and 3 hours after washing their faces on the 2nd, 4th, 6th, 8th and 10th days after using the samples. The average of the two measurements (1 and 3 hours after washing the face) was taken as the measured value, and the sebum content when not washing with the samples was set to 0.

[0187] 4. Test Results

[0188] The test results are shown in Table 5.

[0189] Table 5 Cortical content test results

[0190]

[0191] As can be seen from Table 5, the samples in group A, group C, group D and group E all have an inhibitory effect on skin sebum secretion, among which the samples in group A have the best inhibitory effect on sebum secretion, and compared with the samples in group C, group D and group E, the effect of group A on inhibiting sebum secretion has been greatly improved, indicating that the components of the anti-hair loss, oil-control and antibacterial composition of the present invention have a synergistic effect on inhibiting sebum secretion.

[0192] Embodiment 25

[0193] In this embodiment, the antibacterial performance of the anti-hair loss oil control and antibacterial composition is tested in the following steps:

[0194] 1. Sample Preparation

[0195] The strains used in the experiment included Escherichia coli, Staphylococcus aureus, Clostridium tetani, Propionibacterium acnes, Pseudomonas aeruginosa, Candida albicans, Salmonella and Staphylococcus aureus, all of which were from the strain center of Beijing Institute of Biological Products Identification. The strains used in the experiment were inoculated in nutrient medium (LB medium, Nantong Kaiheng Biotechnology Co., Ltd.), activated at 37°C for 12h, and then made into 5mL / L bacterial suspension with sterile water, and placed in a refrigerator at 4°C for use.

[0196] Set up 5 groups of samples, A, B, C, D, and E, and the samples in each group are as follows:

[0197] In group A, the anti-hair loss, oil-control and antibacterial composition prepared in Example 1 was prepared into a solution with a composition mass concentration of 5.0% using deionized water.

[0198] In group B, the Forsythia suspensa extract was prepared into a solution with a mass concentration of 3.0% using deionized water.

[0199] In group C, the Sophora flavescens extract was prepared into a solution with a mass concentration of 3.0% using deionized water.

[0200] In group D, the Platycladus orientalis leaf extract was prepared into a solution with a mass concentration of 5.0% using deionized water.

[0201] In group E, the wormwood leaf extract was prepared into a solution with a mass concentration of 3.0% using deionized water.

[0202] 2. Determination of antibacterial properties

[0203] The filter paper diffusion method was used to make filter paper into small circular pieces of 6 mm. The small circular filter paper pieces were attached to LB culture medium (LB culture medium was prepared into agar plates according to the conventional method and sterilized by high-pressure steam sterilization) coated with 5 mL / L bacterial suspension. For each experimental bacterial species, 5 μL of samples from groups A to E were taken and dropped onto each small circular filter paper piece respectively. Sterile water was added as a control. The plates were then placed in a 37°C constant temperature incubator for 24 h, the bacterial growth was observed, and the diameter of the inhibition zone was measured.

[0204] 3. Test results

[0205] The test results are shown in Table 6.

[0206] Table 6 Antibacterial zone diameter test results

[0207]

[0208] It can be seen from Table 6 that compared with the samples in group B, group C, group D and group E, the samples in group A have better antibacterial effect, indicating that the components of the anti-hair loss, oil-control and antibacterial composition of the present invention also have a synergistic effect in antibacterial performance.

[0209] Embodiment 26

[0210] Existing studies have shown that Malassezia is the main factor causing dandruff and scalp itching. The reason is that Malassezia is lipophilic and uses the fatty substances secreted in the hair follicles of the human scalp as food, leaving some digested fat on the scalp, which becomes a irritant for dandruff (see "Research Status and Prospects of Plant Anti-dandruff and Antipruritic Agents", Daily Chemicals Science, Vol. 34, No. 2, February 2011). If shampoo or conditioner contains a substance that can effectively inhibit the rapid growth of Malassezia, the problem of dandruff and scalp itching can be alleviated. In this embodiment, the inhibitory effect of the anti-hair loss oil control and antibacterial composition of the present invention on Malassezia is investigated, and the steps are as follows:

[0211] 1. Sample Preparation

[0212] The anti-hair loss, oil-control and antibacterial composition prepared in Example 1 was prepared into a solution with a composition mass concentration of 5.0% with deionized water, and then sterilized at 115° C. for 20 minutes and stored. Before use, it was diluted 10 times with Sandcastle liquid culture medium (product of Amresco, USA), that is, the concentration of the composition was 0.5% when used.

[0213] 2. Strain Preparation

[0214] Malassezia (ATCC strain, USA) was subcultured twice. On the fourth day of the second subculture, the suspension was washed with sterile saline to prepare a bacterial suspension. The number of bacteria in the suspension was counted by a cell counter and the number of bacteria was 1 to 2 × 10 9 / L, diluted 10 times with Shabao liquid medium before inoculation.

[0215] 3. Inoculation and Cultivation

[0216] Add 100 μL of the bacterial suspension prepared in step 2 above to each well of the 96-well plate, and then divide the 96-well plate into 2 groups, each with 48 wells. For the sample group, add 100 μL of sample to each well; for the control group, add 100 μL of Sandcastle liquid culture medium to each well. Place the inoculated 96-well plate in a humidified chamber and culture at 34°C for 24 to 72 hours.

[0217] 4. Results

[0218] The bacterial solution in each well of the 96-well plate cultured in step 2 was counted using a cell counter, and the average number of bacteria in the control group was taken as 1. The average number of bacteria in the sample group was calculated relative to the number of bacteria in the control group as shown in Table 7.

[0219] Table 7

[0220]

[0221] As can be seen from Table 7, the sample group has a good inhibitory effect on Malassezia, indicating that the anti-hair loss, oil-control and antibacterial composition of the present invention can effectively inhibit the growth of Malassezia.

Claims

1. An anti-hair loss, oil-control and antibacterial composition, characterized in that: The composition consists of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract. The Platycladus orientalis leaf extract is obtained by continuously circulating extraction of dried Platycladus orientalis leaves with an ethanol aqueous solution, the Forsythia suspensa extract is obtained by continuously circulating extraction of dried Forsythia suspensa with an ethanol aqueous solution, the Sophora flavescens extract is obtained by extracting dried Sophora flavescens with a supercritical CO2 extraction method, and the Artemisia argyi extract is obtained by extracting dried Artemisia argyi with a supercritical CO2 extraction method. In the composition, the mass ratio of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract is 0.5:0.5:3:2:1:

1.

2. The anti-hair loss, oil-control and antibacterial composition according to claim 1, characterized in that: The preparation method of the Platycladus orientalis leaf extract is as follows: using an ethanol aqueous solution with an ethanol volume percentage of 70% to 75% as an extractant, performing continuous cyclic extraction on dried Platycladus orientalis leaf powder under normal pressure, controlling the weight ratio of Platycladus orientalis leaf powder to the extractant to be 1:(5 to 10), and the extraction time to be 2 to 4 hours, and distilling and concentrating the obtained extract until the volume fraction of ethanol is less than 5%, thereby obtaining the extract; the preparation method of the Forsythia suspensa extract is as follows: using an ethanol aqueous solution with an ethanol volume percentage of 90% to 95% as an extractant, performing continuous cyclic extraction on dried Forsythia suspensa powder under normal pressure, controlling the weight ratio of Forsythia suspensa powder to the extractant to be 1:(3 to 5), and the extraction time to be 2 to 4 hours, and distilling and concentrating the obtained extract until the volume fraction of ethanol is less than 5%, thereby obtaining the extract.

3. The anti-hair loss, oil-controlling and antibacterial composition according to claim 1, characterized in that: The preparation method of the Sophora flavescens extract is as follows: placing dried Sophora flavescens powder in an extraction kettle, extracting for 4 to 6 hours under the conditions of an extraction temperature of 40°C, an extraction pressure of 32MPa, and a CO2 flow rate of 20 to 25kg / h, to obtain the extract; the preparation method of the Artemisia argyi extract is as follows: placing dried Artemisia argyi powder in an extraction kettle, extracting for 4 to 6 hours under the conditions of an extraction temperature of 45°C, an extraction pressure of 32MPa, and a CO2 flow rate of 20 to 25kg / h, to obtain the extract.

4. The anti-hair loss, oil-control and antibacterial composition according to any one of claims 1 to 3, characterized in that: The molecular weight of the hydrolyzed keratin is ≥100,000 Daltons.

5. An anti-hair loss, oil-control and antibacterial shampoo, characterized in that: The shampoo contains the anti-hair loss, oil control and antibacterial composition according to any one of claims 1 to 4, and the mass fraction of the anti-hair loss, oil control and antibacterial composition in the shampoo is 0.5% to 10%.

6. The anti-hair loss, oil-controlling and antibacterial shampoo according to claim 5, characterized in that: The shampoo is composed of the following components in percentage by mass:

7. The anti-hair loss, oil-controlling and antibacterial shampoo according to claim 6, characterized in that: The surfactant is at least two of ammonium laureth sulfate, ammonium lauryl sulfate, sodium laureth sulfate, sodium laureth carboxylate, sodium lauroyl sarcosinate, potassium lauroyl glycinate, sodium lauryl sulfate, and sodium laureth sulfosuccinate; the softening conditioner is dimethicone, dimethiconol, aminodimethicone, aminobispropyl dimethicone, cyclopentasiloxane, polyquaternium-10, polyquaternium-7, polyquaternium-47, PEG-7 glyceryl cocoate, guar hydroxypropyltrimonium chloride, polyethylene glycol-90M, polyethylene glycol-14M, polyquaternium-16 The invention relates to a novel polyol having a plurality of monomers: a copolymer of at least two of ethylene glycol-7M; the thickener is at least two of cocoyl alkylamide, cocamide DEA, cocamide MEA, cocamide methyl MEA, cocamide MIPA, cocamidopropyl betaine, lauryl betaine, lauryl hydroxysulfobetaine, lauryl glucoside, laurin glyceride, lauryl lactate, sodium lauroamphoacetate, cetearyl alcohol, cetyl alcohol, xanthan gum, carbomer, polyacrylic acid, hydroxyethyl cellulose and hydroxypropyl methylcellulose; the acid-base regulator is at least one of citric acid, sodium citrate, lactic acid, sodium lactate, ethylenediaminetetraacetic acid and sodium salt of ethylenediaminetetraacetic acid.

8. An anti-hair loss, oil-controlling and antibacterial hair conditioner, characterized in that: The hair conditioner contains the anti-hair loss, oil-controlling and antibacterial composition according to any one of claims 1 to 4, and the mass fraction of the anti-hair loss, oil-controlling and antibacterial composition in the hair conditioner is 0.3% to 5%.

9. The anti-hair loss, oil-controlling and antibacterial hair conditioner according to claim 8, characterized in that: The conditioner is composed of the following components in percentage by mass:

10. The anti-hair loss, oil-controlling and antibacterial hair conditioner according to claim 9, characterized in that: The hair conditioning agent is behenyltrimethylammonium chloride, behenyltrimethylammonium sulfate, stearyltrimethylammonium chloride, cetrimonium chloride, polydimethylsiloxane, cyclopentasiloxane, polydimethylsiloxane alcohol, aminopropyl polydimethylsiloxane, aminoterminated polydimethylsiloxane, polyquaternium-10, polyquaternium-7, guar hydroxypropyltrimonium chloride, polyethylene glycol-14M, polyethylene glycol-7M, polyethylene glycol-90 M; the thickener is at least one of cetyl alcohol, cetearyl alcohol, behenyl alcohol, ceteareth-25, glyceryl stearate, polyquaternium-37, ceteareth-20, xanthan gum, acrylic acid polymer, hydroxyethyl cellulose, and hydroxypropyl methylcellulose; the acid-base regulator is at least one of citric acid, sodium citrate, lactic acid, sodium lactate, ethylenediaminetetraacetic acid, and sodium salt of ethylenediaminetetraacetic acid.

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