Preparation containing Tibetan medicine musk, preparation method of preparation and application of preparation in preparation of liver cancer medicine

Abstract

The invention relates to the technical field of Tibetan medicines, in particular to a preparation containing Tibetan medicine musk, a preparation method of the preparation and application of the preparation in preparation of liver cancer medicines. The preparation is prepared from the following raw materials: musk, swertia mussotii, rheum tanguticum, dracocephalum tanguticum, delphinium delphinium, phyllanthus emblica, salviae miltiorrhizae, herpetospermum pedunculosum, saussurea hypoglauca, saffron crocus, trogopterus dung, fructus chebulae and plantago asiatica. According to the invention, a plurality of medicinal materials are compatible, all the raw materials have a synergistic effect, the effects of compound components are effectively exerted, and the traditional Chinese medicine composition can resist cancer, protect liver, clear away heat and toxic materials and inhibit tumor growth, has a good anti-liver cancer effect and does not generate an immunosuppressive effect on a body.

Description

Technical Field

[0001] The invention relates to the technical field of Tibetan medicine, and more particularly discloses a preparation containing Tibetan medicine musk, a preparation method thereof, and application of the preparation in preparing liver cancer medicine. Background Art

[0002] Liver cancer (HCC) is a malignant tumor that originates in the liver parenchyma or intrahepatic bile duct epithelial cells. It includes hepatocellular carcinoma, intrahepatic cholangiocarcinoma, and mixed hepatocellular and intrahepatic cholangiocarcinoma. It is one of the most common cancers worldwide, with an estimated 500,000 to 1 million new cases annually. It ranks second among all malignant tumors in terms of mortality. The underlying etiology of HCC remains unclear. Studies have shown that HCC is associated with obesity, diabetes, hepatitis, and other conditions. HCC is particularly common in certain regions of Asia and sub-Saharan Africa, with an annual incidence as high as 50,000 cases per 100,000 population. Currently, the main treatments for HCC include radiotherapy, chemotherapy, and surgery. However, radiotherapy and chemotherapy have drawbacks such as significant side effects and the inability to differentiate between normal and tumor cells. Surgery is prone to recurrence, and chemical drugs are expensive to develop and have significant side effects.

[0003] Tibetan medicine, a component of China's vast medical heritage, boasts a long history and rich content. It embodies the Tibetan people's invaluable experience in combating illness, possessing a unique theoretical system and strong ethnic characteristics. Formed in the seventh century AD, Tibetan medicine has been influenced by diverse medical schools, including Chinese medicine from mainland China, ancient Indian Ayurvedic medicine, and ancient Arabic Unani medicine. While preserving its own traditional characteristics, it has creatively developed a new medical system. Throughout the course of human civilization, the emergence and development of Tibetan medicine have inevitably possessed a strong vitality. As human interaction and communication continue to grow, its theories and practices have garnered global attention and recognition.

[0004] Tibetan medicine considers liver and gallbladder diseases to be classified as "triba diseases," with the root cause being the gallbladder. Triba diseases are primarily caused by improper diet (e.g., salty or sour foods), unclean food, indigestion, and anger. Once the disease develops, it affects the gallbladder and spreads throughout the body. The Four Medical Classics states that under physiological conditions, the human body's triba is divided into five main categories: digestive triba, color-changing triba, active triba, vision triba, and color-clear triba. Digestive triba resides in the stomach, containing digested and undigested food. It is responsible for digestion, breaking down food into its essence and waste, generating heat to warm the body, and enhancing the functions of the other four types of triba. Color-changing triba, located in the liver, changes the color of the essence, transforming it into blood to nourish the entire body. Existing research on Tibetan medicines for liver diseases has primarily focused on single herbs, with limited research on Tibetan medicinal formulas and the active ingredients and compatibility of compound preparations. Consequently, the utilization of active ingredients in medicinal materials is insufficient.

[0005] Therefore, how to provide a preparation containing Tibetan medicine musk and its preparation method and application in the preparation of liver cancer drugs is a problem that needs to be solved urgently by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides a preparation containing Tibetan medicine musk, a preparation method thereof, and application of the preparation in preparing liver cancer drugs.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] A preparation containing Tibetan medicine musk comprises the following raw materials: musk, Swertia vulgaris, Rheum tanguticum, Orchidaceae, Delphinium cyanus, Phyllanthus emblica, Salvia miltiorrhiza, Melon seeds, Saussurea dwarfis, Saffron, Pentaxylorhizoma Cibotii, Terminalia chebula, and Plantago.

[0009] Preferably, the raw materials are included in parts by weight: 200-300 parts of musk, 200-300 parts of swertia paniculata, 150-220 parts of tangut rhubarb, 150-220 parts of lanzensis, 140-180 parts of blue phlox, 120-150 parts of emblica, 100-110 parts of salvia miltiorrhiza, 90-95 parts of melon seeds, 90-95 parts of dwarf saussurea, 75-85 parts of saffron, 60-80 parts of aconiti lobata, 35-50 parts of terminalia chebula, and 25-45 parts of plantain.

[0010] Preferably, the raw materials are as follows, calculated by weight: 270 parts of musk, 270 parts of swedewort, 180 parts of rhubarb tanguticum, 180 parts of orchid, 165 parts of blue phlox, 140 parts of emblica, 105 parts of salvia miltiorrhiza, 92 parts of melon seeds, 92 parts of dwarf saussurea, 80 parts of saffron, 75 parts of aconiti lobata, 40 parts of terminalia chebula, and 35 parts of plantain.

[0011] As an inventive concept identical to the above technical solution, the present invention also claims protection for a method for preparing the above preparation, comprising the following steps:

[0012] (1) Weigh each raw material according to the above parts by weight;

[0013] (2) musk, swertia chuanxiensis, rhubarb tanguticum, orchid of Ganqing, and blue delphinium flower are dried, crushed in a ball mill, and passed through a 100-200 mesh sieve to obtain a powder, the obtained powder is soaked in an ethanol aqueous solution, heated and extracted, and then homogenized, and filtered under reduced pressure to obtain a filtrate;

[0014] (3) Drying the emblica fruit, salvia miltiorrhiza, melon seeds, dwarf safflower, crocus, aconite root, terminalia chebula, and plantain, grinding them in a ball mill, passing through a 100-150 mesh sieve to obtain a powder, adding an ethanol aqueous solution to the obtained powder, performing microwave-assisted extraction, filtering, and concentrating under reduced pressure to a relative density of 1.05-1.1 to obtain an extract;

[0015] (4) The filtrate and the extract are mixed evenly, and freeze-dried to obtain a preparation.

[0016] Preferably, the mass ratio of the powder to the ethanol aqueous solution in step (2) is 1:12-18, the volume concentration of the ethanol aqueous solution is 60-75%, and the soaking time is 1-2 hours.

[0017] Preferably, the temperature of the heating extraction in step (2) is 75-90° C. and the time is 3-6 hours.

[0018] Preferably, the homogenization treatment in step (2) is performed at a pressure of 250 to 400 MPa and for a time of 5 to 10 minutes.

[0019] Preferably, the mass ratio of the powder to the ethanol aqueous solution in step (3) is 1:10-15, and the volume concentration of the ethanol aqueous solution is 60-75%.

[0020] Preferably, the power of the microwave in step (3) is 500-8000W, the extraction temperature is 60-80°C, and the extraction time is 2-4h.

[0021] As an inventive concept identical to the above technical solution, the present invention also seeks protection for the use of the above preparation in the preparation of a drug for treating liver cancer.

[0022] Musk: Invigorates the mind, invigorates blood circulation, relieves pain and swelling, warms and nourishes the liver and kidneys, enhances liver and kidney function, improves blood circulation in the liver and kidneys, and improves the body's immunity.

[0023] Sichuan Swertia japonica: anti-tumor, clears heat and detoxifies, reduces liver damage.

[0024] Tangut Rhubarb: attacks accumulation and relieves stagnation, clears fire and cools blood, activates blood circulation and removes blood stasis.

[0025] Ganqinglan: soothes the stomach and relieves liver, clears liver heat, and stops bleeding.

[0026] Blue Delphinium: anti-tumor, anti-inflammatory and analgesic.

[0027] Phyllanthus emblica: clears heat and cools blood, protects the liver, detoxifies and reduces swelling.

[0028] Salvia miltiorrhiza: Anti-inflammatory and analgesic, removes blood stasis and promotes new blood circulation.

[0029] Boluo melon seeds: clear away heat and detoxify, reduce fire and fever.

[0030] Saussurea dwarf: detoxifies, clears heat from the pulse.

[0031] Saffron: It protects the liver, protects the liver, and promotes bile secretion. It can reduce the increase of transaminase caused by various reasons, and improve the degeneration, necrosis and inflammation of liver cells.

[0032] Wulingzhi: clears heat, detoxifies, nourishes the body, and strengthens bones.

[0033] Terminalia chebula: It has a scavenging effect on active oxygen, removes toxins from the body, relieves the burden on the liver, promotes liver repair, improves liver function, enhances the liver's disease resistance, inhibits liver cell inflammation, and enhances the liver's anti-toxin ability.

[0034] Plantago: It helps to promote liver detoxification and also promotes liver metabolism.

[0035] It can be seen from the above technical solution that, compared with the prior art, the present invention provides a preparation containing Tibetan medicine musk, a preparation method thereof, and an application thereof in the preparation of liver cancer drugs, including the following beneficial effects:

[0036] (1) The present invention combines a variety of medicinal materials, and the raw materials synergize to enhance the efficacy, effectively exerting the effects of the compound ingredients, and can fight cancer and protect the liver, clear away heat and detoxify, and inhibit tumor growth. It has a good anti-liver cancer effect and will not produce an immunosuppressive effect on the body.

[0037] (2) The present invention rationally extracts each raw material, promotes standardization of the preparation process, and improves the utilization rate of the active ingredients. The high-pressure homogenization process improves the uniformity of the compound preparation, accelerates the dissolution of the active ingredients, avoids damage to the active ingredients, and improves the extraction efficiency. In addition, the ingredients are natural, biocompatible, and have few side effects, making it suitable for long-term use. DETAILED DESCRIPTION

[0038] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0039] Example 1

[0040] A preparation containing Tibetan medicine musk comprises the following raw materials, measured by weight: 200 parts of musk, 200 parts of swertia ternata, 150 parts of rhubarb tanguticum, 150 parts of lanzensis, 140 parts of blue delphinium flowers, 120 parts of emblica fruit, 100 parts of salvia miltiorrhiza, 90 parts of melon seeds, 90 parts of saussurea dwarfis, 75 parts of saffron, 60 parts of aconiti lobata, 35 parts of terminalia chebula, and 25 parts of plantain.

[0041] The preparation method of the above preparation comprises the following steps:

[0042] (1) Weigh each raw material according to the above parts by weight;

[0043] (2) musk, swertia chuanxiensis, rhubarb tanguticum, orchid of Ganqing, and blue delphinium flower are dried, crushed in a ball mill, and passed through a 100-mesh sieve to obtain a powder, the obtained powder is soaked in an ethanol aqueous solution, heated and extracted, and then homogenized, and filtered under reduced pressure to obtain a filtrate;

[0044] (3) Drying the emblica fruit, salvia miltiorrhiza, melon seeds, dwarf safflower, crocus, aconite root, terminalia chebula, and plantain, grinding them in a ball mill, passing through a 100-mesh sieve to obtain a powder, adding an ethanol aqueous solution to the obtained powder, performing microwave-assisted extraction, filtering, and concentrating under reduced pressure to a relative density of 1.05 to obtain an extract;

[0045] (4) The filtrate and the extract are mixed evenly, and freeze-dried to obtain a preparation.

[0046] The mass ratio of the powder to the ethanol aqueous solution in step (2) is 1:12, the volume concentration of the ethanol aqueous solution is 60%, and the soaking time is 1 hour.

[0047] The heating extraction temperature in step (2) is 75° C. and the time is 3 h.

[0048] The homogenization process in step (2) was carried out under a pressure of 250 MPa and for 5 minutes.

[0049] The mass ratio of the powder to the ethanol aqueous solution in step (3) is 1:10, and the volume concentration of the ethanol aqueous solution is 60%.

[0050] The power of the microwave in step (3) is 500W, the extraction temperature is 60°C, and the extraction time is 2h.

[0051] Example 2

[0052] A preparation containing Tibetan medicine musk comprises the following raw materials, measured by weight: 300 parts of musk, 300 parts of swertia japonica, 220 parts of rhubarb tanguticum, 220 parts of lanzensis, 180 parts of blue delphinium flowers, 150 parts of emblica, 110 parts of salvia miltiorrhiza, 95 parts of melon seeds, 95 parts of saussurea dwarfis, 85 parts of saffron, 80 parts of aconiti lobata, 50 parts of terminalia chebula, and 45 parts of plantain.

[0053] The preparation method of the above preparation comprises the following steps:

[0054] (1) Weigh each raw material according to the above parts by weight;

[0055] (2) musk, swertia chuanxiensis, rhubarb tanguticum, orchid of Ganqing, and blue delphinium flower are dried, crushed in a ball mill, and passed through a 200-mesh sieve to obtain a powder, the obtained powder is soaked in an ethanol aqueous solution, heated and extracted, and then homogenized, and filtered under reduced pressure to obtain a filtrate;

[0056] (3) Drying the emblica fruit, salvia miltiorrhiza, melon seeds, dwarf safflower, crocus, aconite root, terminalia chebula, and plantain, grinding them in a ball mill, passing through a 150-mesh sieve to obtain a powder, adding an ethanol aqueous solution to the obtained powder, performing microwave-assisted extraction, filtering, and concentrating under reduced pressure to a relative density of 1.1 to obtain an extract;

[0057] (4) The filtrate and the extract are mixed evenly, and freeze-dried to obtain a preparation.

[0058] The mass ratio of the powder to the ethanol aqueous solution in step (2) is 1:18, the volume concentration of the ethanol aqueous solution is 75%, and the soaking time is 2 hours.

[0059] The temperature of the heating extraction in step (2) is 90° C. and the time is 6 hours.

[0060] The homogenization process in step (2) is carried out at a pressure of 400 MPa and for a time of 10 min.

[0061] The mass ratio of the powder to the ethanol aqueous solution in step (3) is 1:15, and the volume concentration of the ethanol aqueous solution is 75%.

[0062] The power of the microwave in step (3) is 8000W, the extraction temperature is 80°C, and the extraction time is 4h.

[0063] Example 3

[0064] A preparation containing Tibetan medicine musk comprises the following raw materials, measured by weight: 270 parts of musk, 270 parts of swertia chuanxiensis, 180 parts of rhubarb tanguticum, 180 parts of lanzensis chinensis, 165 parts of delphinium blue flower, 140 parts of emblica fruit, 105 parts of salvia miltiorrhiza, 92 parts of melon seeds, 92 parts of saussurea dwarfis, 80 parts of saffron, 75 parts of aconiti lobata, 40 parts of terminalia chebula, and 35 parts of plantain.

[0065] The preparation method of the above preparation is the same as that of Example 2.

[0066] Comparative Example 1

[0067] A preparation containing Tibetan medicine musk, which differs from Example 3 in that 270 parts of musk and 270 parts of Swertia sichuanensis are replaced by 540 parts of musk, and the rest are the same.

[0068] Comparative Example 2

[0069] A preparation containing Tibetan medicine musk, which differs from Example 3 in that 270 parts of musk and 270 parts of Swertia sichuanensis are replaced by 540 parts of Swertia sichuanensis, and the rest are the same.

[0070] Comparative Example 3

[0071] A preparation containing Tibetan medicine musk, which differs from Example 3 in that 270 parts of musk are replaced by 270 parts of water, and the rest are the same.

[0072] Comparative Example 4

[0073] A preparation containing Tibetan medicine musk, which differs from Example 3 in that high-pressure homogenization treatment is not performed in step (2) of the preparation method, and the rest are the same.

[0074] Animal experiments

[0075] The SMMC-7721 tumor cell line used in the present invention was purchased from Beina Chuanglian Biotechnology Co., Ltd. in the mall, with the number BNCC338089. Preparation of tumor cell homogenate suspension: SMMC-7721 tumor-bearing mice that were growing vigorously and without ulceration 10 days after inoculation were placed in a 75% ethanol aqueous solution and killed by asphyxiation. The tumor mass was removed under sterile conditions, the capsule and necrotic part were removed, and sterile physiological saline was added at a ratio of 1:3. The suspension was placed in a glass homogenizer and ground evenly to prepare a suspension containing approximately 1.5×10 tumor cells. 7 / mL SMMC-7721 tumor cell suspension.

[0076] A solid tumor mouse model was established using a Kunming strain, clean-grade mice weighing 18-22 g. A suspension of SMMC-7721 tumor cells was inoculated subcutaneously in the right forelimb axilla at a rate of 0.2 mL per mouse. The mice were weighed 24 hours after inoculation and randomly divided into groups of 10 mice each, including a model group, a positive control group, Example groups 1-3, and Comparative Example groups 1-4. The positive control group received 100 mg / kg of 5-fluorouracil, while the Example and Comparative Example groups received 10 g / kg of the formulation. The model group received an equal volume of normal saline. Administration was by gavage once daily for 10 consecutive days.

[0077] After administration, the mice were weighed and sacrificed. The tumor masses were dissected and weighed. Spleen and thymus tissues were also removed and weighed, and the spleen index and thymus index were calculated. The results are shown in Table 1. The calculation formula is as follows:

[0078] Spleen index = spleen weight / body weight × 100%; thymus index = thymus weight / body weight × 100%.

[0079] Table 1 Tumor weight, spleen and thymus index

[0080] Group Tumor weight (g) Spleen index Thymus index Model Group 1.38 52.43 16.61 Positive control group 0.59 33.92 8.12 Example 1 0.72 52.06 15.46 Example 2 0.69 55.24 16.17 Example 3 0.63 58.47 18.69 Comparative Example 1 0.95 43.56 12.04 Comparative Example 2 1.03 40.28 11.65 Comparative Example 3 1.14 38.84 10.73 Comparative Example 4 0.88 47.73 13.25

[0081] Analysis shows that the preparation of the present invention has a significant inhibitory effect on mouse tumor growth, and at the same time does not reduce the spleen index and thymus index of mice, indicating that it will not produce an immunosuppressive effect on the body, helps patients recover, and has a positive effect on overall health. Although the positive control group can inhibit tumors, it will also reduce its own immunity. Compared with chemical drugs, the present invention avoids side effects. After the key components were removed from the control group, the tumor inhibition effect was significantly reduced, proving that musk and Swertia japonica have synergistic effects, which is very critical to the efficacy of the formula, and the amount of raw materials used is also very important. The therapeutic effect of Example 3 is the best. High-pressure homogenization treatment can accelerate the dissolution of active ingredients, avoid damage to active ingredients, further improve extraction efficiency, and enhance efficacy.

[0082] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0083] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A preparation containing Tibetan medicine musk, characterized in that: Including the following ingredients: Musk, Swertia vulgaris, Rhubarb tangutica, Orchidaceae, Delphinium, Phyllanthus emblica, Salvia miltiorrhiza, Melon seeds, Saussurea dwarfis, Saffron, Pentaxyl, Terminalia chebula, Plantago.

2. A preparation containing Tibetan medicine musk according to claim 1, characterized in that, The invention comprises the following raw materials in parts by weight: 200-300 parts of musk, 200-300 parts of swertia paniculata, 150-220 parts of rhubarb tanguticum, 150-220 parts of orchid of Ganqing, 140-180 parts of blue phlox, 120-150 parts of emblica fruit, 100-110 parts of salvia miltiorrhiza, 90-95 parts of melon seeds, 90-95 parts of dwarf saussurea, 75-85 parts of saffron, 60-80 parts of aconiti lobata, 35-50 parts of terminalia chebula, and 25-45 parts of plantain.

3. A preparation containing Tibetan medicine musk according to claim 2, characterized in that, The invention comprises the following raw materials in parts by weight: 270 parts of musk, 270 parts of swertia paniculata, 180 parts of rhubarb tanguticum, 180 parts of orchid of Ganqing, 165 parts of blue phlox, 140 parts of emblica, 105 parts of salvia miltiorrhiza, 92 parts of melon seeds, 92 parts of dwarf saussurea, 80 parts of saffron, 75 parts of aconiti lobata, 40 parts of terminalia chebula and 35 parts of plantain.

4. The method for preparing a preparation containing Tibetan medicine musk according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Weigh each raw material according to the above parts by weight; (2) musk, swertia chuanxiensis, rhubarb tanguticum, orchid of Ganqing, and blue delphinium flower are dried, crushed in a ball mill, and passed through a 100-200 mesh sieve to obtain a powder, the obtained powder is soaked in an ethanol aqueous solution, heated and extracted, and then homogenized, and filtered under reduced pressure to obtain a filtrate; (3) Drying the emblica fruit, salvia miltiorrhiza, melon seeds, dwarf safflower, crocus, aconite root, terminalia chebula, and plantain, grinding them in a ball mill, passing through a 100-150 mesh sieve to obtain a powder, adding an ethanol aqueous solution to the obtained powder, performing microwave-assisted extraction, filtering, and concentrating under reduced pressure to a relative density of 1.05-1.1 to obtain an extract; (4) The filtrate and the extract are mixed evenly, and freeze-dried to obtain a preparation.

5. The method for preparing a preparation containing Tibetan medicine musk according to claim 4, wherein: The mass ratio of the powder to the ethanol aqueous solution in step (2) is 1:12-18, the volume concentration of the ethanol aqueous solution is 60-75%, and the soaking time is 1-2 hours.

6. The method for preparing a preparation containing Tibetan medicine musk according to claim 4, characterized in that: The temperature of the heating extraction in step (2) is 75-90° C. and the time is 3-6 hours.

7. The method for preparing a preparation containing Tibetan medicine musk according to claim 4, characterized in that: The homogenization treatment in step (2) is performed at a pressure of 250 to 400 MPa and for a time of 5 to 10 minutes.

8. The method for preparing a preparation containing Tibetan medicine musk according to claim 4, characterized in that: The mass ratio of the powder to the ethanol aqueous solution in step (3) is 1:10-15, and the volume concentration of the ethanol aqueous solution is 60-75%.

9. The method for preparing a preparation containing Tibetan medicine musk according to claim 4, characterized in that: The power of the microwave in step (3) is 500-8000W, the extraction temperature is 60-80°C, and the extraction time is 2-4h.

10. Use of the preparation according to any one of claims 1 to 3 in the preparation of a medicament for treating liver cancer.