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Sterilization composite containing phenazino-1-carboxylic acid

A technology of Shenzimycin and its composition, which is applied in the field of bactericidal composition, can solve the problems of decreased activity, powdery mildew effect is not as good as before, etc.

Active Publication Date: 2013-06-19
SHENZHEN NOPOSION AGROCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A new compound has an excellent effect initially, but its activity tends to decline to a certain extent after long-term and single use
For example, there have been reports of resistance to kresoxim-methyl in many parts of the world, but the effect on powdery mildew is not as good as before

Method used

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  • Sterilization composite containing phenazino-1-carboxylic acid

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] Bioassay Example 1: Indoor Toxicity Determination of Shenzimycin and Kresoxim-methyl Compound to Rice Sheath Blight

[0026] Test object: Rice sheath blight (Rhizoctonia solani), collected from the field, separated and purified indoors.

[0027] Test method: Refer to "People's Republic of China Agricultural Industry Standard NY / T1156.5-2006", adopt broad bean leaf method. Choose susceptible faba bean potted plants, cut leaves with petioles in the same part, with the same growth, keep them moisturized, and place them in a petri dish. Dissolve the original drug with acetone, dilute with 0.1% Tween 80 water, and prepare 6 series of mass concentrations. Fully soak the leaves in the pre-configured medicinal solution for 5 seconds, drain off the excess medicinal solution, and after natural air-drying, use an inoculator to inoculate the side of the bacterium cake with a diameter of 5mm and the mycelium in the center of the treated leaves, and inoculate 30 leaves for each trea...

example 2

[0036] Biological Assay Example 2: Indoor Toxicity Determination of Shenzimycin and Azoxystrobin Compound on Watermelon Fusarium Wilt

[0037] Test object: watermelon wilt (Fusarium oxysporum f.sp. Niveum, FON), collected from the field, separated and purified indoors.

[0038] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1156.2-2006", mycelium growth rate method.

[0039] The test method and statistical method are the same as the biological assay example 1, and the middle inhibitory concentration EC is calculated by the least square method 50 , and then calculate the co-toxicity coefficient (CTC) according to Sun Yunpei's method. When CTC120, the composition exhibits synergistic effect.

[0040] The results of the toxicity test are shown in Table 2.

[0041] Table 2 Indoor toxicity test results of Shenzimycin and azoxystrobin compound against watermelon wilt

[0042] deal with

example 3

[0043] Bioassay Example 3: Indoor Toxicity Determination of Shenzimycin and Trifloxystrobin Compound on Cucumber Blight

[0044] Test object: cucumber blight (Mycosphaerella melonis (Pass.) Chiu et Walker), collected from the field, separated and purified indoors.

[0045] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1156.2-2006", mycelium growth rate method. Dissolve the original drug with acetone, dilute with 0.1% Tween 80 water, and prepare 6 series of mass concentrations. Under sterile conditions, take 5 mL of the prepared medicinal solution and mix them with 75 mL of culture medium evenly to make 4 plates containing medicine, and mix an equal amount of sterile water with the culture medium as a control. Under aseptic conditions, the cultured cucumber blight was cut out from the edge with a sterilized puncher with a diameter of 5 mm. Use an inoculator to inoculate the fungus cake on the center of the drug-containing plate, with the...

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Abstract

The invention relates to sterilization composite containing phenazino-1-carboxylic acid. The effective components are one type of phenazino-1-carboxylic acid (A) or methoxy acrylate composite (B), wherein the mass ratio between the effective components (A) and (B) is 50:1-1:50. The sterilization composite is mainly used for preventing and treating fungous diseases of cucurbits and vegetables, apples and field crops.

Description

Technical field [0001] The invention relates to a bactericidal composition, the active ingredients of which are shenzimycin (A) and a methoxyacrylate compound (B). Background technique [0002] Shenzimycin is a fermentation product of Pseudomonas fluorescens strain M18. Its main component is phenazine-1-carboxylic acid. It is effective against rice sheath blight, cucumber vine blight, watermelon wilt, etc. It has good inhibitory effects on a variety of plant pathogenic bacteria. Shenzimycin has been carried out large-scale promotion trials in Shanghai, Hubei, Hainan, Henan, Gansu and other places, and has shown good field effects on rice sheath blight, apple spotted leaf disease, watermelon fusarium wilt, melon vine blight, etc. Prevention effect. [0003] Methoxyacrylate compounds are excellent agricultural fungicides that mainly interfere with the respiration of pathogenic bacteria’s mitochondria and inhibit cellular energy flow. This type of fungicides mainly includes ...

Claims

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Application Information

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IPC IPC(8): A01N43/60A01N37/50A01N43/54A01N43/40A01N37/36A01N47/24A01P3/00
Inventor 唐彩乐曹明章张华东王新军赵娜李广泽
Owner SHENZHEN NOPOSION AGROCHEM
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