Method for ecological control of citrus Huanglongbing by using composite microbial agent

A compound microbial inoculum and a technology for citrus huanglongbing, which is applied in the field of agricultural management, can solve the problems of ecological environment damage, inconvenient for large-scale application, and difficult to achieve large-scale whole-scale eradication of citrus huanglongbing, and achieves no chemical residues, convenient The effect of large-scale application

Inactive Publication Date: 2020-02-28
刘文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are at least the following deficiencies in the existing technology: (1) extermination and disease control are out of touch, which is one of the reasons why Huanglongbing cannot be cured
(2) The combined control of chemical pesticides and chemical fungicides or microbial agents will cause poor results due to mutual exclusion and offset
(3) Chemical pesticides are basically used. As the resistance of pests and bacteria increases, more and more chemical pesticides are used, resulting in a serious increase in pesticide residues and non-point source pollution, and damage to the ecological environment.
Chemical pesticides only act on the surface and cannot kill the bacteria deep inside the plant, which is one of the reasons why citrus greening disease cannot be cured at present
(4) Because it is harmful to humans, livestock, poultry and natural enemies of pests, pollutes the ecological environment, and has chemical residues in food, the amount of use should be limited, which is not convenient for large-scale application, and it is difficult to achieve large-scale eradication of citrus Huanglongbing.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for the ecological prevention and treatment of citrus huanglongbing with compound microbial bacterial agent comprises the following steps:

[0034] S1, preparation of compound microorganism A preparation: liquid Beauveria bassiana, liquid Metarhizium anisopliae, liquid Bacillus subtilis, liquid Bacillus licheniformis, liquid Bacillus thuringiensis, adsorption carrier by mass ratio : 1:1:1:1:1:5 to mix evenly to obtain a composite microorganism A preparation with a moisture content ≥ 15%;

[0035] S2. Preparation of composite microorganism B preparation: the liquid Trichoderma viride agent, the liquid Paecilomyces lavender agent, the liquid Bacillus subtilis agent, the liquid Bacillus licheniformis agent, the liquid Bacillus thuringiensis agent, and the adsorption carrier in a mass ratio of 2 : 2: 1: 1: 1: 3 to mix evenly to obtain a composite microorganism B preparation with a moisture content ≥ 15%;

[0036] S3. Applying the compound microbial preparation: ...

Embodiment 2

[0059] The method for ecologically preventing and controlling citrus Huanglongbing with compound microbial inoculum comprises the following steps:

[0060] S1. Preparation of compound microorganism A preparation: liquid Beauveria bassiana inoculum, liquid Metarhizium anisopliae inoculum, liquid Bacillus subtilis inoculum, liquid Bacillus licheniformis inoculum, liquid Bacillus thuringiensis inoculum, and adsorption carrier are in a mass ratio of : 3: 3: 2: 2: 2: 3 and mix evenly to obtain a composite microorganism A preparation with a moisture content of ≥ 15%;

[0061] S2, preparation of compound microorganism B preparation: liquid Trichoderma viride agent, liquid lilac Paecilomyces agent, liquid Bacillus subtilis agent, liquid Bacillus licheniformis agent, liquid Bacillus thuringiensis agent, adsorption carrier according to mass ratio is 3 : 3: 2: 2: 3: 5 for uniform mixing to obtain a composite microorganism B preparation with a moisture content of ≥ 15%;

[0062] S3. Appl...

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PUM

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Abstract

The invention discloses a method for ecological control of Citrus Huanglongbing by using a composite microbial agent. The method includes the following steps: S1. preparing a composite microbial agentA; S2. preparing a compound microbial agent B; S3. applying a compound microbial agent, wherein the compound microbial agent A and the compound microbial agent B can be applied simultaneously or separately. The method uses combined beneficial microbial biocontrol bacteria to infect and inactivate diaphorina citri pests and cut off the infectious source of Citrus Huanglongbing; and the method usesthe combined microbial beneficial bacteria of antagonistic citrus Huanglong harmful germ-liberibacter to impregnate soil, root system, branches and leaves, citrus bast and branches and leaves, occupy, entangle and inactivate germs, repair soil with residual agrochemical toxicity and bacteria, create a good environment of green, environmental protection and ecological restoration, and prevent andcure the Citrus Huanglongbing.

Description

technical field [0001] The invention relates to the technical field of agricultural management, in particular to a method for ecologically controlling citrus huanglongbing with a compound microbial agent. Background technique [0002] Citrus huanglongbing is caused by the infection of citrus huanglongbing pathogenic bacteria - Phloem bacillus, which can cause devastating diseases to citrus planting. This pathogen is mainly transmitted by citrus psyllids. Generally, the disease occurs when the citrus tree is 2-3 years old. Once the disease is difficult to cure, it will not only waste years of hard work by the fruit grower, but also spend energy to dig out the whole diseased tree and destroy it. Citrus huanglongbing is difficult to cure and occurs for a long time, causing serious losses to growers. In Guangxi Autonomous Region alone, citrus Huanglongbing disasters occurred in 71 cities and counties in 2018, causing a loss of about 500,000 tons of citrus and an economic loss ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01G17/00A01B79/02A01N63/30A01N63/36A01N63/38A01N63/22A01N63/20A01P1/00A01P7/04C12N1/20C12N1/14C12N11/14C12R1/645C12R1/07C12R1/10C12R1/125C12R1/885C12R1/79
CPCA01B79/02A01G13/00A01G17/005A01N63/00C12N1/14C12N1/20C12N11/14
Inventor 刘文韩莉华刘海龙
Owner 刘文
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