Diaphorina citri Kuwayama high pathogenic Isaria fumosorosea strain and application thereof

A technology of Cordyceps rosacea and citrus psyllid, applied in the field of biological control, can solve problems such as excessive pesticide residues in fruits, loss of biodiversity, pollution of orchard ecological environment, etc., and achieve strong infection and insecticidal effect and strong application. potential effect

Active Publication Date: 2016-11-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of citrus psyllids is still dominated by traditional chemical control, but the long-term and large-scale use of these chemical pesticides has led to a large number of natural enemy insects in citrus orchards, loss of biodiversity, and different degrees of resistance of citrus psyllids. , the ecological environment of the orchard is polluted and other problems, and the problem of excessive pesticide residues in fruits also occurs from time to time

Method used

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  • Diaphorina citri Kuwayama high pathogenic Isaria fumosorosea strain and application thereof
  • Diaphorina citri Kuwayama high pathogenic Isaria fumosorosea strain and application thereof
  • Diaphorina citri Kuwayama high pathogenic Isaria fumosorosea strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and identification of Ifusia fumoscens strain IF-BDC01

[0020] 1. Source of materials

[0021] (1) Sample: I. fumoscens was preserved by the Biological Control Engineering Research Center of the Ministry of Education of South China Agricultural University. The preserved sample of this strain was purified by single cell isolation.

[0022] (2) Potato dextrose agar medium (PDA): 200g peeled potatoes, cut into small pieces, add 1L of water, boil for 20min, filter with gauze: add 20g of glucose while hot, 18-20g of agar, stir to dissolve and mix, set Fill it into a Erlenmeyer flask and sterilize it in an autoclave (121°C, 30min) for 30min.

[0023] (3) Sterile operating conditions: All utensils and utensils must be sterilized in a pressure cooker (121°C, 30min), and inoculation and other operations are carried out in an ultra-clean workbench.

[0024] (4) Culture conditions: culture in a 25°C light (12L:12D) incubator. After the colony is formed, tran...

Embodiment 2

[0051] Example 2 Determination of the pathogenicity of the strain IF-BDC01 against young nymphs and advanced nymphs of the citrus psyllid

[0052] 1. Bioassay is one of the effective means to detect the lethality and lethality rate of entomogenic fungi to target pests, and can provide an important reference for comprehensive evaluation of the biological control potential of entomogenic fungi. In this study, IF-BDC01 strain IF-BDC01 was tested for its pathogenicity to young and old nymphs of citrus psyllids, in order to screen out the optimal age and concentration for its lethality to the two stages of citrus psyllids.

[0053] 2. Insects and host plants for testing: citrus psyllids. Adults of citrus psyllids were raised in the net room of the Biological Control Engineering Research Center of the Ministry of Education, and inoculated on clean and healthy Jiulixiang. Each plant of Jiulixiang was inserted with about 20 pairs of adults. A net made of PVC film was used to cover eac...

Embodiment 3

[0076] Example 3 Field control of I. fumigatus IF-BDC01 strain on citrus psyllid adults

[0077] 1. Treat adults of citrus psyllids with the spore suspension of I. fumoscens under semi-enclosed conditions. The cumulative mortality on the 7th day is shown in Table 8. With the increase in the concentration of I. fusca spores, lethal The rate is getting bigger and bigger.

[0078] Table 8 Mortality of I. fumosmos to adult citrus psyllids under semi-field conditions

[0079]

[0080] Note: The same letter in the same column in the table means that the difference is not significant at the 0.05 level (DMT method)

[0081] 2. The daily mortality rate of I. fumoscens to citrus psyllids in the field

[0082] The insecticidal effect of entomopathogenic fungi was relatively slow. After spraying, the citrus psyllids began to die on the 3rd day. After that, the mortality of citrus psyllid adults in the treatments of various concentrations continued to increase, and increased with the ...

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Abstract

The invention discloses a Diaphorina citri Kuwayama high pathogenic Isaria fumosorosea strain IF-BDC01 and application thereof. The Isaria fumosorosea strain IF-BDC01 is preserved in Guangdong Microbiology Culture Center on March 1, 2016 with a preservation number of GDMCC No:60011, and the preservation address is building 59, 100th yard on middle martyr road, Guangzhou. Long-term infection biology and indoor biological determination proves that the Isaria fumosorosea strain IF-BDC01 has very high pathogenicity on Diaphorina citri Kuwayama and very strong infecting insecticidal effect. The strain is an entomopathogenic fungus, and has very strong application potential as an in vivo biopesticide in biological control of Diaphorina citri Kuwayama.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a highly pathogenic strain of I. fumosmosus of citrus psyllid and its application. Background technique [0002] Citrus psyllid ( Diaphorinacitri Kuwayama) is an important insect vector of citrus huanglongbing, which poses a huge potential threat to the healthy development of citrus industry in my country and the world. The young shoots of the damaged host may wither and deform the new shoots. Psyllids also secrete white honeydew and adhere to branches and leaves, which can cause the occurrence of soot disease. To make matters worse, psyllids feed on citrus Huanglongbing diseased plants, lay eggs and reproduce, producing a large number of infected adults, which can spread Huanglongbing by transferring and injuring new plants. The main damage crops of citrus psyllids are Rutaceae plants, among which Citrus suffers the most, followed by Pseudomonas, M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 邱宝利郭长飞代晓彦任顺祥
Owner SOUTH CHINA AGRI UNIV
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