Diaphorina citri Kuwayama parasitism natural energy scale breeding method

A citrus psyllid, parasitic technology, applied in the field of insect breeding, can solve problems that are difficult to implement and difficult to implement

Inactive Publication Date: 2015-05-20
GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology is not suitable for small and medium-sized farms. It is difficult to implem...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Reproduction and production of citrus psyllid gnats

[0017] 1. A greenhouse: Gourilla is grown and processed in a greenhouse. Purchasing Jiulixiang seedlings in the market, about 30 cm high, after pruning, soak them in 5% bleaching powder aqueous solution for 10 minutes to remove insects and germs on the plants, and obtain about 2000 clean Jiulixiang without disease and insect infection.

[0018] 2. Two insect breeding rooms:

[0019] (1) The first entomology room is divided into two parts, one part is for cultivating citrus psyllids, and the other part is used for preparation and treatment of parasitoid hosts. The conditions in the insectarium are light 10:14 (L:D), temperature 22-28°C . In the previous part, the clean and pest-infested Murata was used in the greenhouse, and the citrus psyllids were inserted into it, and they continued to multiply, and a large number of citrus psyllids were obtained, and they were supplemented at any time according to the...

Embodiment 2

[0027] Embodiment 2 Reproduction and production of citrus psyllid Agnaces

[0028] 1. Two greenhouses:

[0029] (1) The first greenhouse for planting and processing Murata. Jiulixiang seedlings are purchased in the market, about 30cm high, after pruning, spray vegetable oil insecticide (95% vegetable oil or waste oil) to dilute 20 times, remove insects and germs on the plants, and obtain about 2000 clean plants without pests Infected Gourilla.

[0030] (2) In the second greenhouse, the relative control temperature in the greenhouse is 22-28 degrees Celsius, with natural light. Move a large number of Murata into the first greenhouse at one time, and insert citrus psyllids, gradually cultivate a large number of citrus psyllids, and supplement Murata or citrus psyllids at any time as needed.

[0031] 2. One insect cage: there are 6-8 small insect cages and 10 large insect cages. The culture conditions in the insect culture room are: light L:D=10:14, temperature 22-28 degrees ...

Embodiment 3

[0039] Embodiment 3 The propagation and production of citrus psyllid jumping wasp

[0040]1. Two greenhouses:

[0041] (1) The first greenhouse for planting and processing Murata. Gourita seedlings are purchased on the market, about 30cm high, after pruning, spray rotenone to remove insects and germs on the plants, and obtain about 2000 clean Gourita without disease and insect infection.

[0042] (2) In the second greenhouse, the relative temperature in the greenhouse is controlled at 22-28 degrees Celsius, with natural light. Move a large number of citrus psyllids into the first greenhouse at one time, and inoculate them with citrus psyllids, gradually cultivate a large number of citrus psyllids, and supplement them with citrus psyllids at any time as needed.

[0043] 2. One insect cage: there are 6-8 small insect cages and 10 large insect cages.

[0044] (1) Each small insect cage is put into 1-2 strains of the first greenhouse's clean and free of diseases and insects. W...

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Abstract

The invention discloses a diaphorina citri Kuwayama parasitism natural energy scale breeding method. Insects and germs on a Murraya exotica plant are eliminated to obtain a clean Murraya exotica without disease and pest infection. The clean Murraya exotica without the disease and pest infection is transferred to a first greenhouse or a first insect rearing cage, and the diaphorina citri Kuwayama is grafted to breed. Under normal lighting conditions, the Diaphorina citri Kuwayama is cultured and bred under the temperature of 22 to 28 DEG C, and the Murraya exotica or the Diaphorina citri Kuwayama is supplemented at any moment according to requirements. The clean Murryaya exotica without the disease and pest infection is transferred to a second greenhouse or a second insect rearing cage, and the Diaphorina citri Kuwayama in the first greenhouse or the first insect rearing cage is grated. Under the normal lighting conditions, the Diaphorina citri Kuwayama is cultured to spawn and grow to a nymph of 4-5 years old under the temperature of 22 to 28 DEG C. The parasitic wasp and parasitic Diaphorina citri Kuwayama are utilized for inoculation, and the parasitic wasp can be colleted after 7 to 10 days. According to the Diaphorina citri Kuwayama parasitism natural energy scale breeding method, the industrialization production of the Diaphorina citri Kuwayama parasitic wasp is achieved.

Description

Technical field: [0001] The invention belongs to the field of insect breeding, and in particular relates to a method for large-scale breeding of parasitic natural enemies of citrus psyllids. Background technique: [0002] Citrus Huanglongbing (HLB) is a destructive disease that occurs in many major citrus producing countries such as China, the United States, and Brazil, and poses a serious threat to the world's citrus production. In recent years, citrus huanglongbing has spread continuously in some citrus producing areas in my country, causing serious losses to some areas. The citrus psyllid Diaphorina citri Kuwayama is the only insect vector of citrus HLB, and its control is considered to be the key to control HLB. In recent years, researchers have found that the control of citrus psyllid in citrus production still has many problems in the critical period and methods of control. The inventor of this patent has put forward some suggestions (Mao Runqian et al., 2014). It i...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 郑基焕毛润乾郭洋洋张宇宏
Owner GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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