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Diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and identification primer and identification method thereof

A citrus psyllid, specific technology, applied in the fields of biochemistry and molecular biology

Active Publication Date: 2013-01-16
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is still a lack of effective methods for the quantitative evaluation of predatory natural enemies preying on citrus psyllids in the field. How to use molecular biology techniques to qualitatively and accurately quantitatively detect the predation efficiency of predatory natural enemies on citrus psyllids, and accurately analyze the predation efficiency of citrus psyllids. Spectrum of sexual natural enemies will be the key to technological breakthroughs in pest control

Method used

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  • Diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and identification primer and identification method thereof
  • Diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and identification primer and identification method thereof
  • Diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and identification primer and identification method thereof

Examples

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Embodiment 1

[0014] Example 1: Acquisition and application of citrus psyllid-specific DNA molecular marker sequences

[0015] Genomic DNA of each species was extracted by single head homogenate method

[0016] Referring to the method of An Ruisheng et al. (An Ruisheng, Tan Shengjiang, Chen Xiaofeng. Improvement of the homogenization method for DNA extraction of small insects. Insect Knowledge, 2002,39(4):311-312), the single-headed insects were washed with double distilled water, Blot dry and put into a 1.5mL centrifuge tube, add 50μL extraction buffer A [1%SDS, 50mmol / L Tris Cl, 25mmol / LNaCl, 25mmol / L EDTA], place at -20°C for 5min, mash with a grinding rod, and then use Rinse the grinding rod with 100 μL of extraction buffer; bathe in water at 65°C for 45 minutes, take out and mix twice in the middle; add an equal volume of extraction solution B [3mol / L KAc (pH7.2)], place on ice for more than 1 hour; centrifuge at 12000r / min for 10 minutes , transfer the supernatant to another centrifu...

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Abstract

The invention discloses diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and an identification primer and an identification method thereof. The diaphorina citri specific molecular marker DNA sequence is indicated as SEQ ID No. 1. The identification primer of the diaphorina citri specificity molecular marker DNA sequence is indicated as SEQ ID No. 2 and No. 3. The problems that traditional observation methods researching predators to predation effect of pests and biochemical methods researching relationships between predators and preys are complex, high in cost, lack of specificity of target and the like are solved, diaphorina citri specific molecular marker DNA sequence and the identification primer and the identification method thereof are provided, and an efficient method is provided for researching insect predation.

Description

technical field [0001] The invention belongs to the field of biochemistry and molecular biology, and in particular relates to a citrus psyllid-specific molecular marker DNA sequence, an identification primer and an identification method thereof. Background technique: [0002] The citrus psyllid (Diaphorina citri Kuwayama) belongs to the family Psyllidae of the order Homoptera and is the only carrier of the Asian and American species of citrus greening disease. It can carry the virus for life after eating the diseased Huanglong strain, and the transmission rate is high. At present, the control of citrus psyllids in my country mainly adopts two measures: tree cutting and chemical control. Although chemical control of citrus psyllids has certain effects, the frequent use of pesticides will damage the ecological environment and cause serious pesticide resistance problems that cannot be ignored. Therefore, it is an urgent task to find new prevention and control methods and appr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 孟翔欧阳革成郭明昉方小端卢慧林
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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