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Paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control

A technology for Paecilomyces lilacinus and Bemisia tabaci, applied to Paecilomyces lilacinus PlTS01 and its application field in the control of Bemisia tabaci, can solve the problems of no reports on the prevention and control of Bemisia tabaci, and achieve good promotion and application Value, the effect of sustainable development promotion

Active Publication Date: 2016-01-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] To sum up, it can be seen that there are many invention patents for the use of Paecilomyces lilacinus to control nematodes, and a small number of patent applications for the control of pests, but there is no report on the application of Paecilomyces lilacinus to control whitefly

Method used

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  • Paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control
  • Paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control
  • Paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and screening of Paecilomyces lilacinus strain PlTS01

[0031] The soil was collected from Zhongchuan Town, Yongdeng County, Gansu Province. The soil samples were sieved to remove stones and sundries. Take 10g of pure soil and suspend in 90mL of 0.05% Tween 80 solution. Shake well, let stand for 15min, and take the supernatant 2mL was diluted in 8mL0.05% Tween 80.

[0032] Selective culture: Inoculate 0.1mL of the above suspension on the surface of the selective medium plate and spread evenly with a triangular glass rod. At 25°C, culture at constant temperature for 3-6 days, then use an inoculation loop to pick mycelium from a single colony, inoculate it on a PDA plate to continue culturing, and store it in a refrigerator at 4°C after purification.

[0033] After the above operations, a fungus with pathogenic effect on Bemisia tabaci was screened out from the soil, numbered PlTS01.

Embodiment 2

[0034] Example 2 Identification of strain PlTS01

[0035] The bacterial strain PlTS01 screened in Example 1 was on the Chapei medium, initially the colony was regular and round, raised, with white hyphae, and the back was light yellow or nearly beige; after prolonged cultivation, the colony was still round and formed a pale pink spore layer; After 14 days of culture, the colony diameter was 44~46mm.

[0036] Microstructure: mycelium is long and thin, colorless and transparent, with septa, width 1.58~3.61μm; 2~5 phialides grow on the conidiophores, phialides are oval or fusiform inflated, gradually thin tube upward, 6.07~7.44μm long, 2.77~3.66μm wide; a large number of conidia are produced on it, the conidia are strung together, colorless and transparent, and the single cells are oval to oval, 2.51~3.66μm long, 1.77~2.66μm wide μm.

[0037] Molecular phylogeny: The rDNA-ITS gene sequence of the PlTS01 strain (as shown in SEQ ID NO: 1) was amplified with universal primers IT...

Embodiment 3

[0041] Preparation of conidia suspension: pick conidia from the slant strains, insert them into 0.05% Tween-80 solution that has been sterilized, measure the concentration of spores with a hemocytometer, and adjust to 10 7 conidia / mL. spare.

[0042] Seed culture: inoculate 2mL spore suspension in 98mL seed medium, at 180r / m and 26 0 Shake flask culture under C condition for 2-3 days and serve as seed solution. Seed medium is the Czapek-Dox medium of 0.5% peptone, and its composition is: albumen 5g, NaN0 3 3g, K 2 HP0 4 1g, MgS0 4 7H 2 00.5g, KCl0.5g, FeS0 4 7H 2 00.01g, molasses 30g, agar 15-20g, water 1000mL.

[0043] Solid fermentation: steam the rice first, put it into a fresh-keeping bag, and inoculate the above seed liquid after it is cooled to normal temperature, the inoculation ratio is 5-15% (V / W), and the inoculation ratio is 5-15% (V / W), at 25-28 0 C culture for about 2 weeks, until the surface of the rice grains is covered with conidia, the conidia are sepa...

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Abstract

The present invention belongs to the technical field of biological pesticides, and specifically discloses paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control. The paecilomyces lilacinus PlTS01 strain is isolated and purified from soil in Zhongchuan town Yongdeng County in Gansu Province, and is preserved in China Center for Type Culture Collection (CCTCC) on March 31, 2015, and the accession number is CCTCC No. M2015168. The paecilomyces lilacinus PlTS01 strain has strong bemisia tabaci insecticidal activity, sixth day after the treatment, medial lethal concentration LC50 of B-type bemisia tabaci second instar nymphs is 1.66 * 10<6> spores / mL, and the median lethal time LT50 is 4.35 d (at a dose of 1.0 *10<7> spores / mL). The paecilomyces lilacinus PlTS01 strain shows good prospects for being used as an environmentally friendly microbial pesticide in control of the agricultural important pest-bemisia tabaci.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a strain of Paecilomyces lilacinus PlTS01 and its application in controlling Bemisia tabaci. Background technique [0002] whitefly Bemisiatabaci (Gennadius) is a small insect with piercing-sucking mouthparts, and it is also a rapidly evolving compound species. Its biotypes B and Q belong to the world's major pests. Since the 1980s, with the worldwide trade, Bemisia tabaci has spread widely and caused disasters all over the world with the help of flowers, seedlings and other economic crops. At present, Bemisia tabaci has been widely distributed in Asia, More than 90 countries and regions in Europe, America, Oceania, Africa, etc. have brought serious economic losses to global agricultural production. In my country, Bemisia tabaci has spread to every province, city and autonomous region except Tibet, seriously affecting agricultural production. [0003] Bemisia tabaci is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/79
Inventor 胡琼波张博文翁群芳姜艳芳
Owner SOUTH CHINA AGRI UNIV
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