Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci

A technology of Corynesium fumigatus and Bemisia tabaci, which is applied to high-efficiency Corynesporium species and its application in the control of Bemisia tabaci, can solve the problems of large-scale application of fungal insecticides with unstable control effects, etc. To achieve the effect of non-toxicity for humans and animals

Active Publication Date: 2013-05-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the control effect of existing entomopathogenic fungi to control whitefly is unstable and other reasons limit the l

Method used

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  • Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci
  • Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci
  • Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In August 2012, the inventors collected naturally susceptible Bemisia tabaci carcasses, separated and purified strains from them, measured pathogenicity, and carefully compared biological characteristics and production characteristics. After strain identification, repeated screening , Obtained a strain with high efficiency for Bemisia tabaci. The classification status of the strain belongs to Eumycota, Mitosporic fungi, Hyphomycetes, Hyphomycetales, and Hyphomycetales. Family (Hyphomycetaceae), Corynespora ( Isaria ). No sexual generation of this strain was found.

[0023] Said strain ( Isaria fumosoroseus ) IfB01 has been deposited in the China Type Culture Collection (CCTCC), the preservation date is October 12, 2012, and the preservation number is CCTCC M 2012400.

[0024] The morphological characteristics of the Corynespora fumigatus strain: on Cha's medium, the colonies are initially regular and round, and the hyphae are white; extended culture, the colonies are irre...

Embodiment 2

[0026] Example 2 Conidia production

[0027] Preparation of spore suspension: pick out the conidia from the slant strain, connect it to 0.05% Tween-80 solution that has been sterilized, measure the spore concentration with a hemocytometer, and adjust it to 10 7 Conidia / mL. spare.

[0028] Seed culture: Inoculate 2 mL of spore suspension in 98 mL of seed culture medium, and culture it in a shake flask at 180 r / m and 26°C for 2 to 3 days to obtain seed liquid. The seed medium is 0.5% peptone Czapek-Dox medium, and its composition is: protein aging 5 g, NaN0 3 3 g, K 2 HP0 4 1 g, MgS0 4 · 7H 2 0 0.5 g, KCl 0.5 g, FeS0 4 · 7H 2 0 0.01 g, 30 g of saccharose, 15-20 g of agar, 1000 mL of water.

[0029] Solid fermentation: first steam the rice, put it in a fresh-keeping bag, and after it cools to room temperature, inoculate the above seed liquid, the inoculation ratio is 5-15% (V / W, mL / g), at 25-28℃ After culturing for about 2 weeks, when the surface of the rice grains grows out of c...

Embodiment 3

[0030] Example 3 Controlling Bemisia tabaci by Corynespora fumigatus

[0031] Using the conidia produced in Example 2 above, under laboratory conditions, the spore suspension was inoculated to B. tabaci nymphs on potted kale seedlings, and the incidence of Bemisia tabaci at different times after treatment was investigated The results show that this strain has a very good effect on whitefly nymphs, and the half-lethal concentration for 2nd instar whitefly nymphs is 7.5×10 4 days after inoculation. 5 Conidia / mL, 7.8×10 for the 3rd instar Bemisia tabaci nymph 5 1 conidia / mL, as shown in Table 1.

[0032] Use the conidia produced in the above example 2 to control Bemisia tabaci in the field. During the peak period of Bemisia tabaci nymphs, 1×10 7 30 L / mu of conidia / mL conidia suspension was sprayed on B-type Bemisia tabaci on open-field cultivated tomatoes, and the infestation rate of Bemisia tabaci was investigated at different times after application. The results showed that this st...

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Abstract

The invention relates to isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci. The isaria fumosoroseus is preserved in China Center For Type Culture Collection, wherein the preserving data is 12th, October, 2012, and the preserving number is CCTCC M2012400. The taxonomic status belongs to Eumycota, Mitosporic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetaceae and Isaria. The isaria fumosoroseus is highly pathogenic to B type bemisia tabaci, and the medial lethal concentration to two-year old nymph (LC50) is 7.5*10<5> conidia/mL (the fourth day after inoculation), and the field control efficiency to B type bemisia tabaci is over 90% which is equal to that of chemical insecticide spirotetramat. The isaria fumosoroseus serving as a living biological insecticide is free from environmental pollution and toxicity to human and livestock, and is suitable for production of organic agricultural products.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a high-efficiency Corynesporium sp. and its application in preventing and treating Bemisia tabaci. Background technique [0002] whitefly Bemisia tabaci (Gennadius) is a worldwide important agricultural and forestry pest, which causes serious economic losses. There are dozens of biotypes of Bemisia tabaci, among which type B and type Q are the most dangerous pests. Type B bemisia invaded my country in the 1990s, and then Q type bemisia also invaded our country. These two biotypes have widely harmed the main agricultural and forestry crops in most parts of our country, and have caused tens of billions of dollars. Economic losses. Bemisia tabaci has a wide range of hosts, many biotypes, and many generations per year. It is easy to form drug resistance, so it is extremely difficult to control. [0003] Bemisia tabaci is a polyphagous pest with a wide r...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 胡琼波邹春华
Owner SOUTH CHINA AGRI UNIV
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