Indoor subculture breeding method of phenacoccus solenopsis tinsley

A technology of hibiscus hibiscus and net room, which is applied in the field of insect breeding, can solve the problems of population degradation, inability to subculture hibiscus hibiscus for a long time, and inability to achieve effective reproduction of hibiscus hibiscus, and achieves maintaining vitality and good effect. Effect

Active Publication Date: 2020-11-10
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no long-term subculture plan for indoor long-term subculture of P. hibiscus, the population is seriously degraded, and it is impossible to achieve effective reproduction of P. fuso.
[0003] Through the above analysis, the existing problems and defects of the existing technology are: currently there is no plan for indoor long-term subculture of P. hibiscus, the population is seriously degraded, and the effective reproduction of P. fuso cannot be achieved.

Method used

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  • Indoor subculture breeding method of phenacoccus solenopsis tinsley
  • Indoor subculture breeding method of phenacoccus solenopsis tinsley
  • Indoor subculture breeding method of phenacoccus solenopsis tinsley

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Embodiment 1

[0077] Collect the wild-grown hibiscus plants parasitized by P. hibiscus cotton, remove other insects or diseased plants, and cultivate the host plant in an outdoor net room; record after laying eggs. After the eggs are first hatched, use a soft brush to pick the newly hatched nymphs gently or adhere to the leaves of the host plant; spread wet filter paper on the bottom of the beaker; cultivate ghost needles as the host plant of the 1st instar nymphs, and put ghost needles The leaves were cut off and processed, and then placed in a beaker; the newly hatched nymphs of Pythococcus hibiscus cotton were grafted on the treated leaves of Pygnus chinensis, and the opening of the beaker was sealed with mesh; the beaker was placed in an incubator for cultivation, The 2nd instar nymphs were obtained after the newly hatched nymphs of P. hibiscus cotton had molted their skins; put the fresh and clean single leaf bark next to the 2nd instar nymphs that had just molted, let them climb to the...

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Abstract

The invention belongs to the technical field of insect breeding, and discloses an indoor subculture breeding method of phenacoccus solenopsis tinsley. The method comprises the following steps of collecting and bringing back plants damaged by the phenacoccus solenopsis tinsley in the wild, and carrying out outdoor net room culture on the plants; after egg laying and primary incubation of the phenacoccus solenopsis tinsley, lightly or adhesively picking newly-incubated nymphs onto leaves of a host plant by using a soft writing brush by taking sticktight as the host plant, cutting off and treating the leaves, and then putting the leaves into a beaker; paving wet filter paper at the bottom of the beaker, and culturing in an incubator; after the newly-incubated nymphs of the phenacoccus solenopsis tinsley are molted, connecting the molted nymphs to fresh and clean single gendarussa ventricosa leaves, and placing the single gendarussa ventricosa leaves in the incubator. According to the method, the phenacoccus solenopsis tinsley on outdoor plants is cultured, obtained eggs are transferred into a room to be cultured, and a proper culture environment can be provided for nymph growth; in the indoor culture process, different culture conditions are set according to the growth period, culture is more targeted, long-term indoor subculture propagation of insects is facilitated, vitality iskept, and development of scientific experiments is facilitated.

Description

technical field [0001] The invention belongs to the technical field of insect breeding, and in particular relates to an indoor subculture breeding method for Pythococcus hibiscus cotton. Background technique [0002] At present: Hibiscus spp. belongs to Homoptera, Pynoacocidae, Pyenococcus subfamily, Pyenococcus genus, originated from North America, and is a vicious invasive pest widely distributed in the world. In the world, it has been distributed to 38 countries and regions; in my country, it is found in 14 provinces and cities including Taiwan. The host range of P. hibiscus is wide, including 207 species of plants in 149 genera in 57 families, covering crops, ornamental plants, vegetables, shrubs, fruit trees and weeds. The piercing-sucking mouthparts are inserted into the phloem or mesophyll to absorb nutrients and inject toxic substances, resulting in chlorosis, dysplasia, distortion, and even death of the host. The excreted honeydew attracts ants and causes the occur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01G22/60A01G22/00
CPCA01K67/033A01G22/60A01G22/00
Inventor 潘志萍翟欣
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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