Screening method of cotton varieties or strains resisting Bemisia tabaci
A technology for cotton varieties and Bemisia tabaci is applied in the field of screening Bt transgenic cotton and conventional cotton varieties that are resistant to Bemisia tabaci. , good accuracy and scientificity, the effect of shortening the time
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[0035] 1. Test method
[0036] (1) The test is carried out in the net room (24 meters long, 3.0 meters wide and 2.0 meters high). Planted (lines) in the net room, a total of 139 cotton varieties (lines). Each variety (line) planted 10 cotton varieties (lines), all repeated 3 times, the cotton cultivation row spacing in the net room was 0.80m, the plant spacing was 0.25m, and the seedling stage could be prevented and controlled 1-2 times seedling aphid;
[0037] (2) A 100-mesh nylon net is placed on the outside of the net to prevent the invasion of pests and natural enemies;
[0038] (3) The adults of Bemisia tabaci were artificially released during the 6-9 leaf stage of cotton growth, with an average of 40-50 adults released per plant. The Bemisia tabaci released were natural populations collected from the field;
[0039] (4) 1500 times liquid of phoxim was sprayed in the net room 7 days before releasing Bemisia tabaci to kill natural enemies;
[0040] (5) Investigate the ...
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