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Method for inducing wing shortening of Sogatella furcifera

A technique for white-backed planthopper and white-backed planthopper is applied in the field of inducing short-winged white-backed planthopper, which can solve the problems of time-consuming and labor-intensive, prolonged feeding period, slow growth of insects, etc. The effect of the acquisition rate

Inactive Publication Date: 2011-07-20
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the breeding process, the short-winged individuals of white-backed planthoppers are mainly improved by properly controlling the breeding temperature and light, but it is difficult to determine the appropriate temperature and light. If the temperature is low, the insects will grow slowly and the breeding cycle will be greatly extended. Time-consuming and labor-intensive, and there are not many short-winged individuals obtained during the breeding process, the effect is not very ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Cultivate rice: The rice seeds are first germinated indoors (temperature 26-30°C, humidity 80-90%), and then sowed outdoors in an insect-free net room for about ten days before use.

[0022] 2) White-backed planthopper: It was originally collected from rice fields in Qingxin County, Guangdong Province, and has been bred for about 20 generations in the net room under natural conditions.

[0023] 3) ZR-515 solution preparation: first dissolve ZR-515 in a small amount of acetone, the ratio of ZR-515, acetone, water (W:V:V) is 100μg: 1μl: 9μl, and then use ddH 2 O to dilute it to the desired concentration of 10 ng / μl. With acetone:ddH 2 The ratio of O=1:10 is mutually soluble and used as the control solution.

[0024] 4) Treatment method: Put the white-backed planthopper nymphs that have just molted into the third instar into the ice box and freeze them for about 1 minute, then quickly take 1 μl of the ZR-515 solution or acetone solution (for control) drops with a pipe...

Embodiment 2

[0028] 1) Cultivate rice: The rice seeds are first germinated indoors (temperature 26-30°C, humidity 80-90%), and then sowed outdoors in an insect-free net room for about ten days before use.

[0029] 2) White-backed planthopper: It was originally collected from rice fields in Qingxin County, Guangdong Province, and has been bred for about 20 generations in the net room under natural conditions.

[0030] 3) Dissolve ZR-515 in a small amount of acetone first, the ratio of ZR-515, acetone, and water (W:V:V) is 100μg: 1μl: 9μl, and then use ddH 2 O to dilute it to the desired concentration of 10 ng / μl. With acetone:ddH 2 The ratio of O=1:10 is mutually soluble and used as the control solution.

[0031] 4) Treatment method: Put the white-backed planthopper nymphs that have just molted into the 4th instar into the ice box and freeze them for about 1 minute, and then quickly take 1 μl of the ZR-515 solution or acetone solution (for control) drops with a pipette gun. on the back o...

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Abstract

The invention relates to a method for inducing the wing shortening of Sogatella furcifera by utilizing an exogenous hormone, namely ZR-515 solution. The adopted technical scheme comprises the following steps of: freezing nymphs of the Sogatella furcifera at 3 to 4 ages, then dripping a small amount of juvenile hormone analogue, namely the ZR-515 solution on the backs of the nymphs, and raising the treated nymphs in a raising container for 2 to 8 days. The invention aims to design a novel, simple and convenient scheme for inducing the wing shortening of the Sogatella furcifera to improve the acquisition of short-winged individuals in the process of raising for experimental research materials, or provide a possible way for applying the method to fields and controlling the migration and dispersion hazard of the Sogatella furcifera in the future.

Description

technical field [0001] The invention relates to a method for using exogenous hormone ZR-515 solution to induce short wings of white-backed planthopper. Background technique [0002] White-backed planthopper ( Sogatella furcifera (Horvath)) belongs to the family Delphacidae of the order Homoptera, and is currently a serious pest to rice in my country and many Asian countries. The nymphs of white-backed planthopper are divided into five instars. The adult stage has two types: long-winged and short-winged. It belongs to the sedentary type, with fast development speed and relatively strong reproductive ability. The proportion of long-winged and short-winged types in the population can change with changes in environmental conditions or different feeding conditions. [0003] A large number of studies have shown that raising the feeding temperature can reduce the formation of short-winged adults of planthoppers, and the use of short light can promote the formation of short-winged...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 王方海张琼秀
Owner SUN YAT SEN UNIV
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