Method for preparing sea-tangle polysaccharide

A technology for kelp polysaccharide and kelp, which is applied in the field of extracting polysaccharides from algae as raw materials, can solve the problems of incompatibility with the development needs of enterprises, inability to meet market demands, large loss of active ingredients, etc., and achieve functional diversification, shortening extraction time, polysaccharide Nutritional and functional effects

Active Publication Date: 2008-03-05
DALIAN POLYTECHNIC UNIVERSITY
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AI Technical Summary

Problems solved by technology

Although these two methods are simple and convenient, the extraction rate of polysaccharides is low, the purity is not high, and the

Method used

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Examples

Experimental program
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Embodiment Construction

[0032] Example 1 Rinse the kelp raw material with water, drain it, soak it in 0.1% citric acid solution for 30 minutes, dry it at 20°C until the water content is less than 4%, then crush it to 100 mesh to obtain kelp powder, add 10 times of anhydrous Ethanol, reflux at 60°C for 3 hours, centrifuge at 4000rpm for 10min to obtain kelp powder mixed with a small amount of ethanol, let the residual ethanol completely evaporate at 20°C, add 10 times of water, ultrasonic treatment for 20min, the action temperature is 60°C, the power is 60W, and then Use 6mol / L hydrochloric acid to adjust the pH value to 4, add 0.1% enzyme activity 3.0×10 4 u / g cellulase and 1.5% enzyme activity 1.0×10 4 u / g pectinase, stirred at 40°C for 1 hour, then adjusted the pH to neutral with 0.5 mol / L potassium hydroxide, put the obtained kelp enzymatic solution in a vacuum extraction device, and controlled the vacuum degree to 85KPa, Extract at a temperature of 40°C for 1 hour, and at the same time inactivat...

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Abstract

The laminarin preparing process includes the technological steps of material treatment, crushing, defatting and decolorizing with alcohol, ultrasonic wall breaking, enzyme treatment and vacuum extracting and vacuum freeze drying. During the material treatment, the kelp material including kelp, waste kelp material and leftover from kelp processing is washed first with water and then with citric acid or acetic acid. During the enzyme treatment, the kelp material is enzymolyzed with cellulose and pectase. The present invention has kelp including kelp waste and leftover best utilized to extract laminarin of high purity in high yield, and the prepared laminarin may be served directly or used in developing other functional foods.

Description

technical field [0001] The invention relates to polysaccharide extraction technology, especially the technology of extracting polysaccharide from algae. Background technique [0002] In recent years, due to the great enrichment and improvement of material civilization, people, especially the urban population, have gradually been threatened by "diseases of modern civilization", such as hyperlipidemia, hypercholesterolemia, arteriosclerosis and other cardiovascular diseases, obesity, gastrointestinal The prevalence of cancer is rising. At the same time, due to the limitations of Western medicine in the treatment of tumors, AIDS, hepatitis and other chronic diseases caused by certain drug-resistant bacteria or viruses, many scholars have turned their attention to naturally occurring substances. Active polysaccharides in algae are favored for their ability to effectively prevent and treat "diseases of modern civilization". [0003] Kelp is rich in nutrition, cheap and can be ea...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 朱蓓薇侯红漫董秀萍李冬梅杨静峰初乐
Owner DALIAN POLYTECHNIC UNIVERSITY
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