Comprehensive utilization method of wheat bran
A technology of wheat bran and raw materials, which is applied in the field of comprehensive utilization of wheat by-products, can solve the problems of incomplete utilization of wheat bran, waste of resources and environment, and many extraction studies, and achieve high industrial application value, large economic benefits, and reasonable extraction process.
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Embodiment 1
[0030] (1) Raw material pretreatment: pass the wheat bran through a 50-mesh sieve;
[0031] (2) Get 30g of raw material under the sieve obtained in step (1), add water by material-liquid ratio 1:4, stir;
[0032] (3) Adjust the feed liquid condition pH=3.5 in step (2), temperature 50 degrees, add pretreatment enzyme Viscozyme 150ul, heat preservation pretreatment 1h;
[0033] (4) adjust the feed liquid pH=6 of step (3), the temperature is 90 degrees, add 0.1mol / L of CaCl 2 10ml, add liquefaction enzyme TSC 300ul, liquefy for 1h, and pass the iodine test, wherein TSC is a liquid enzyme preparation containing α-amylase with strong thermal stability, expressed by genetically modified bacillus (Bacillus) base made. The classification name of the enzyme is 1,4-α-D type glucan hydrolase (EC3.2.1.1), which is a commercially available product;
[0034] (5) adjusting the feed liquid pH of step (4) to be 2.5, the temperature is 40 degrees, and the temperature is kept for 2 hours;
...
Embodiment 2
[0042](1) Raw material pretreatment: pass the wheat bran raw material through a 50-mesh sieve;
[0043] (2) Get the under-sieve raw material 30g that obtains in step (1), add water by material-liquid mass ratio 1:4, stir;
[0044] (3) Adjust the pH of the feed solution in step (2) to 3.5, and the temperature is 50° C., add 150 ul of pretreatment enzyme Viscozyme, and incubate for 1 hour;
[0045] (4) Adjust the pH of the feed solution in step (3) to 6, the temperature is 90°C, and 0.1mol / L of CaCl is added 2 10ml, add liquefaction enzyme TSC 300ul, liquefy for 1h, and pass the iodine test;
[0046] (5) adjusting the pH of the feed liquid in step (4) to be 2.5, the temperature is 40°C, and the temperature is kept for 2 hours;
[0047] (6) Suction filter the feed liquid obtained in step (5), wash twice with 3 parts of water each, and mix the filtrates.
[0048] (7) The liquid obtained in the step (6), with Ca(OH) 2 Adjust to pH = 8, stir for 30 minutes, form a precipitate, ...
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