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A technology of wheat bran and raw materials, which is applied in the field of comprehensive utilization of wheat by-products, can solve the problems of incomplete utilization of wheat bran, waste of resources and environment, and many extraction studies, and achieve high industrial application value, large economic benefits, and reasonable extraction process.
Inactive Publication Date: 2018-02-16
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology
In summary, there are many studies on the extraction of a single component, resulting in inco
Method used
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Effect test
Embodiment 1
[0030] (1) Raw material pretreatment: pass the wheat bran through a 50-mesh sieve;
[0031] (2) Get 30g of raw material under the sieve obtained in step (1), add water by material-liquid ratio 1:4, stir;
[0032] (3) Adjust the feed liquid condition pH=3.5 in step (2), temperature 50 degrees, add pretreatment enzyme Viscozyme 150ul, heat preservation pretreatment 1h;
[0033] (4) adjust the feed liquid pH=6 of step (3), the temperature is 90 degrees, add 0.1mol / L of CaCl 2 10ml, add liquefaction enzyme TSC 300ul, liquefy for 1h, and pass the iodine test, wherein TSC is a liquid enzyme preparation containing α-amylase with strong thermal stability, expressed by genetically modified bacillus (Bacillus) base made. The classification name of the enzyme is 1,4-α-D type glucan hydrolase (EC3.2.1.1), which is a commercially available product;
[0034] (5) adjusting the feed liquid pH of step (4) to be 2.5, the temperature is 40 degrees, and the temperature is kept for 2 hours;
...
Embodiment 2
[0042](1) Raw material pretreatment: pass the wheat bran raw material through a 50-mesh sieve;
[0043] (2) Get the under-sieve raw material 30g that obtains in step (1), add water by material-liquid mass ratio 1:4, stir;
[0044] (3) Adjust the pH of the feed solution in step (2) to 3.5, and the temperature is 50° C., add 150 ul of pretreatment enzyme Viscozyme, and incubate for 1 hour;
[0045] (4) Adjust the pH of the feed solution in step (3) to 6, the temperature is 90°C, and 0.1mol / L of CaCl is added 2 10ml, add liquefaction enzyme TSC 300ul, liquefy for 1h, and pass the iodine test;
[0046] (5) adjusting the pH of the feed liquid in step (4) to be 2.5, the temperature is 40°C, and the temperature is kept for 2 hours;
[0047] (6) Suction filter the feed liquid obtained in step (5), wash twice with 3 parts of water each, and mix the filtrates.
[0048] (7) The liquid obtained in the step (6), with Ca(OH) 2 Adjust to pH = 8, stir for 30 minutes, form a precipitate, ...
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Abstract
The invention provides a comprehensive utilization method of wheat bran, and relates to the technical field of comprehensive utilization of wheat byproducts. The method comprises the following steps:pulverizing wheat bran raw materials, conducting sieving, adding water, conducting pretreatment, adding a liquefying enzyme TSC for heat insulation and liquefying, then adding a CaCl2 solution, continuing the heat insulation for 1-2h, adjusting pH value and temperature of a feed liquid according to the used enzymes, and conducting reaction; conducting solid-liquid separation to obtain a solid 1 and a liquid 1, using a saturated Ca(OH)2 solution to adjust the pH value of the liquid 1, forming precipitates and conducting solid-liquid separation to obtain a solid 2 and a liquid 2; saccharifying the liquid 2 to obtain a glucose solution; subjecting the solid 2 to acid dissolution and separation to obtain a phytic acid solution; and adding water into the solid 1, putting the obtained mixture into a colloid mill for crushing, and conducting enzyme treatment so as to obtain a protein product and a cellulose product. The method has high product yield, realizes the comprehensive utilization ofthe wheat bran, and is green and environmentally friendly.
Description
technical field [0001] The invention belongs to the technical field of comprehensive utilization of wheat by-products, and more specifically relates to a method for comprehensive utilization of wheat bran. Background technique [0002] Wheat bran is the main by-product of wheat processing, which is rich in carbohydrates, proteins, vitamins, minerals, etc., and is widely used in feed industry, food industry, and fermentation industry. With the continuous improvement of people's health awareness, the functional factors in wheat bran have been paid more and more attention. The development and utilization of wheat bran has high social and economic benefits. [0003] Wheat bran mainly contains two types of physiologically active substances, namely active polysaccharides and phenolic compounds. Active polysaccharides mainly refer to wheat dietary fiber. Dietary fiber can reduce the passage time in the small intestine, reduce the absorption of glucose, slow down the hydrolysis o...
Claims
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Application Information
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