Process for converting carbon source to ethylene glycol

Abstract

The invention discloses a method for converting a carbon source into ethylene glycol. The method comprises the following steps: providing a plastid, wherein the plastid comprises gene sequences of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6; implanting the plastid into blue-green bacteria by means of electroporation treatment to obtain modified blue-green bacteria; and providing the carbon source to the modified cyanobacteria, so that the modified cyanobacteria converts the carbon source into ethylene glycol, thereby producing chemicals with high economic value while treating the carbon-containing waste gas.

Description

Technical Field

[0001] This invention relates to a method for converting a carbon source into ethylene glycol, and more particularly, to a method for converting a carbon source into ethylene glycol using cyanobacteria. Background Technology

[0002] Cyanobacteria are autotrophic organisms capable of synthesizing their own nutrients through photosynthesis. To mitigate the greenhouse effect and environmental damage, current technologies utilize the ability of cyanobacteria to fix carbon dioxide into metabolic products, applying them to the production of alcohols and organic acids such as ethanol, butanol, 2,3-butanediol, succinic acid, lactic acid, and isopropenyl. However, there is currently no method for converting a carbon source into ethylene glycol using a single cyanobacterium.

[0003] Ethylene glycol (EG) is the simplest diol in structure and is frequently used to manufacture polyester fibers and polyethylene terephthalate (PET). It is also used in automotive antifreeze, hydraulic brake fluid, and pharmaceutical products, making it an economically valuable chemical. However, existing ethylene glycol production typically involves the oxidation of ethylene oxide (EO), which in turn requires silver-catalyzed ethylene oxidation, resulting in a complex manufacturing process.

[0004] Therefore, how to improve the process and use blue-green bacteria to convert carbon sources into ethylene glycol, so as to produce high-value chemicals while treating carbon-containing waste gas, has become one of the important issues that this business aims to solve. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a method for converting a carbon source into ethylene glycol, which addresses the shortcomings of the prior art.

[0006] To address the aforementioned technical problems, one technical solution adopted by the present invention is to provide a method for converting a carbon source into ethylene glycol. The method includes: providing a plastid comprising gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6; implanting the plastid into cyanobacteria by electroporation to obtain modified cyanobacteria; and providing the carbon source to the modified cyanobacteria, causing the modified cyanobacteria to convert the carbon source into ethylene glycol.

[0007] Furthermore, the genomic DNA of the cyanobacterium has a first gene locus and a second gene locus, with the gene sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 located at the first gene locus, and the gene sequences of SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 located at the second gene locus.

[0008] Furthermore, the modified cyanobacteria have the ability to produce 3-phosphoglycerate dehydrogenase, phosphoserine phosphatase, phosphoserine transaminase, oxaloacetate decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase.

[0009] Furthermore, the cyanobacteria are elongated cyanobacteria.

[0010] Furthermore, the electroporation process is performed at a voltage of 0.5 to 1.5 kV for 2 to 10 mSec.

[0011] Furthermore, the electroporation process further includes adding polyethylene glycol at a concentration of 0.5% to 2%.

[0012] Furthermore, the carbon source is carbon dioxide, glucose, sucrose, fructose, or galactose.

[0013] Furthermore, the plastid is an Escherichia coli plastid.

[0014] Furthermore, the method further includes the step of implanting the plasmid into Escherichia coli for mass production.

[0015] To solve the above-mentioned technical problems, another technical solution adopted by the present invention is to provide a method for converting a carbon source into ethylene glycol, which utilizes modified cyanobacteria to convert the carbon source into serine; wherein the modified cyanobacteria include the gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6.

[0016] Furthermore, the genomic DNA of the cyanobacterium has a first gene locus and a second gene locus, with the gene sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 located at the first gene locus, and the gene sequences of SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 located at the second gene locus.

[0017] Furthermore, the carbon source is carbon dioxide, glucose, sucrose, fructose, or galactose.

[0018] Furthermore, the modified cyanobacteria have the ability to produce oxaloacetate decarboxylase to convert L-serine into ethanolamine.

[0019] Furthermore, the modified cyanobacteria have the ability to produce ethanolamine oxidase to convert the ethanolamine into hydroxyacetaldehyde.

[0020] Furthermore, the modified blue-green bacteria has the ability to produce glycolaldehyde reductase to convert the hydroxyacetaldehyde into ethylene glycol.

[0021] One of the beneficial effects of the present invention is that the carbon source to ethylene glycol conversion provided by the present invention can be achieved by using a technical solution of "the plastid including the gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6" and "implanting the plastid into cyanobacteria by electroporation to obtain modified cyanobacteria", thereby utilizing modified cyanobacteria to convert carbon sources into ethylene glycol, achieving the benefits of carbon reduction while obtaining high-economic-value chemicals.

[0022] To further understand the features and technical content of the present invention, please refer to the following detailed description and accompanying drawings. However, the drawings provided are for reference and illustration only and are not intended to limit the present invention. Attached Figure Description

[0023] Figure 1 This is a flowchart of the method for converting a carbon source into serine according to the present invention.

[0024] Figure 2 This is a schematic diagram of the metabolic pathway of the modified cyanobacteria of the present invention.

[0025] Figure 3 and Figure 4 This is a schematic diagram of the plasm construction of the present invention.

[0026] Figure 5 The calibration curves were measured for different ethylene glycol concentrations.

[0027] Figure 6 A graph showing the results of measuring ethylene glycol secreted by cyanobacteria.

[0028] Figure 7 The graphs show the cell density curves of modified cyanobacteria cultured at different temperatures and the bar graphs showing ethylene glycol production.

[0029] Reference numerals: S101, S102, S103: Steps Detailed Implementation

[0030] The following specific embodiments illustrate the implementation of the method for converting a carbon source into ethylene glycol disclosed in this invention. Those skilled in the art can understand the advantages and effects of this invention from the content disclosed in this specification. This invention can be implemented or applied through other different specific embodiments, and various details in this specification can also be modified and changed based on different viewpoints and applications without departing from the concept of this invention. Furthermore, the accompanying drawings of this invention are for simple illustrative purposes only and are not depictions of actual dimensions; this is stated in advance. The following embodiments will further describe the relevant technical content of this invention in detail, but the disclosed content is not intended to limit the scope of protection of this invention.

[0031] It should be understood that the term "or" as used herein may, as the context dictates, include any combination of one or more of the associated listed items. Unless the context otherwise requires, the term "comprising" should be understood to imply inclusion of one or more of the stated integers or steps, but does not exclude any other integer or step or any other set of integers or steps. In this specification, the terms "comprising," "containing," "including," or "having" are used interchangeably.

[0032] The term "exogenous gene" used in this article, also known as a heterologous gene, refers to a gene or nucleotide fragment that is not derived from the endogenous genome of the host or target cell itself, but is taken from other species or cells, or is artificially synthesized and introduced into the host or target cell through genetic engineering technology.

[0033] See Figure 1 and Figure 2 As shown, the present invention provides a method for converting a carbon source into ethylene glycol, comprising: step S101: providing a plastid; step S102: implanting the plastid into cyanobacteria to obtain modified cyanobacteria; and step S103: providing a carbon source to the modified cyanobacteria, so that the modified cyanobacteria convert the carbon source into ethylene glycol. Figure 2In this context, NADP refers to adenine dinucleotide phosphate; NADPH refers to reduced nicotinamide adenine dinucleotide phosphate; ATP refers to adenosine triphosphate; ADP refers to adenosine diphosphate; PSII refers to photosystem II; PSI refers to photosystem I; Cytb6f refers to cytochrome b6f, which is the core of the light-dependent reactions of aerobic photosynthesis; RuBP refers to ribulose-1,5-bisphosphate; CA refers to carbonic anhydrase; rbs refers to ribosome-binding site; SDC refers to serine decarboxylase; TynA refers to ethanolamine oxidase; and YghD refers to glycolalaldehyde reductase.

[0034] More specifically, in step S101, the plasmid includes the gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6. Therefore, prior to step S101, a step of synthesizing a DNA sequence may be included, whereby an artificially synthesized gene encoding a DNA sequence with recognition suitability for cyanobacteria is used for identification and production of corresponding substances by the cyanobacteria. Specifically, this involves artificially synthesizing gene encodings with recognition suitability for *Synechococcus elongates* PCC7942. Furthermore, the plasmid may be an *E. coli* plasmid, and the plasmid can be inserted into *E. coli* for mass production.

[0035] In step S102, plasmids are implanted into cyanobacteria via electroporation. Electroporation involves applying a current to the cyanobacterial cells for an extremely short time (microseconds to milliseconds), creating a high-voltage, low-capacitance environment. This generates a potential difference in the cell membrane, altering its structure and causing it to compress and thin, creating numerous tiny pores that allow the plasmids to penetrate the cell membrane and enter the cyanobacterial cell. In embodiments of the invention, electroporation is preferably performed at a voltage of 0.5 kV for 10 mSec, more preferably at a voltage of 1.5 kV for 5 mSec, and even more preferably at a voltage of 1.0 kV for 5 mSec, to obtain the maximum colony count.

[0036] Furthermore, to achieve optimal plasm permeability, 0.5 to 2% polyethylene glycol (PEG) can be added in this step, for example, at any concentration between 0.5 and 2%, such as 1.0% or 1.5%. Preferably, the electroporation treatment is performed on cyanobacteria at a voltage value between 0.5 and 1.5 kV, for example, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, or 1.4 kV, for 2 to 10 mSec, for example, 3, 4, 5, 6, 7, 8, or 9 mSec, for any milliseconds between 2 and 10. This invention further investigates the use of a quantitative concentration of 1 x 10⁻⁶ kV in the electroporation treatment. 6 The number of modified cyanobacteria strains was successfully obtained by treating the original cyanobacteria under different voltage and time conditions, as shown in Table 1 below.

[0037] Table 1 (1% PEG added to each group)

[0038] Voltage (kV) Time (mSec) Colony number 0.5 2 6 0.5 5 12 0.5 10 21 1.0 2 19 1.0 5 36 1.0 10 11 1.5 2 17 1.5 5 32 1.5 10 8

[0039] See Figure 3 and Figure 4 As shown, it is noteworthy that the genomic DNA of cyanobacteria has at least a first gene locus and a second gene locus. This invention improves the success rate of gene transformation by placing the gene sequences of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3 at the first gene locus and the gene sequences of SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 at the second gene locus. Furthermore, the complete chromosome constructed through genetic engineering can be confirmed using primers that bind to the NSI or NSII gene locus.

[0040] In an embodiment of the present invention, the gene sequences of SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 can be first placed at the second gene locus, and then the gene sequences of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3 can be placed at the first gene locus using the same transformation strategy. In other words, SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3 can be homologously exchanged with SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 at different gene loci, respectively. In another embodiment of the present invention, the gene sequences of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3 can also be first placed at the first gene locus, and then the gene sequences of SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 can be placed at the second gene locus using the same transformation strategy. That is to say, as long as the gene sequences can be placed at the first and second gene loci respectively, the order of gene sequence placement is not particularly limited.

[0041] After the modified protoplasmic DNA is sequentially transformed into native cyanobacteria, the modified cyanobacteria (SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6) can be obtained by screening with antibiotics (spectinomycin and kanamycin). Specifically, spectinomycin and kanamycin can be used for screening, and the successfully modified cyanobacterial strains can be grown on a solid culture medium (BG11) containing antibiotics. More specifically, because the gene sequences of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3 are respectively placed at the first gene locus, and the gene sequences of SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 are placed at the second gene locus, the success rate of gene transformation is improved.

[0042] In embodiments of the present invention, SEQ ID NO:1 is a gene encoding ethanolamine oxidase (TynA) or a gene having more than 80% sequence identity with SEQ ID NO:1 and possessing TynA activity; SEQ ID NO:2 is a gene encoding glycolaldehyde reductase (YghD) or a gene having more than 80% sequence identity with SEQ ID NO:2 and possessing YghD activity; SEQ ID NO:3 is a gene encoding oxaloacetate decarboxylase (SDC) or a gene having more than 80% sequence identity with SEQ ID NO:3 and possessing SDC activity; SEQ ID NO:4 is a gene encoding 3-phosphoglycerate dehydrogenase (SerA) or a gene having more than 80% sequence identity with SEQ ID NO:4 and possessing SerA activity; SEQ ID NO:5 is a gene encoding phosphoserine phosphatase (SerB) or a gene having more than 80% sequence identity with SEQ ID NO:5 and possessing SerB activity; SEQ ID NO:6 is a gene encoding phosphoserine transaminase (SerC) or a gene having more than 80% sequence identity with SEQ ID NO:1 and possessing TynA activity. NO:6 is a gene with more than 80% sequence identity and SerC activity.

[0043] Therefore, cyanobacteria capable of producing 3-phosphoglycerate dehydrogenase (SerA), phosphoserine phosphatase (SerB), and phosphoserine aminotransferase (SerC) can be screened first using 10 μg / mL zizomycin. Then, cyanobacteria capable of producing oxaloacetate decarboxylase (SDC), ethanolamine oxidase (TynA), and glycolaldehyde reductase (YghD) can be screened from these cyanobacteria using 5 to 10 μg / mL kanamycin.

[0044] Therefore, the modified cyanobacteria that successfully undergo homologous exchange simultaneously possess the ability to produce 3-phosphoglycerate dehydrogenase (SerA), phosphoserine phosphatase (SerB), phosphoserine aminotransferase (SerC), serine decarboxylase (SDC), ethanolamine oxidase (TynA), and glycolaldehyde reductase (YghD). This allows them to convert carbon sources into ethylene glycol and release it extracellularly using a single cyanobacterial bacterium without requiring lysis treatment.

[0045] In detail, the modified cyanobacteria of the present invention retain the characteristic of native cyanobacteria to convert carbon sources into glyceraldehyde-3-phosphate (G3P). Furthermore, the modified cyanobacteria of the present invention can also produce 3-phosphoglycerate dehydrogenase (SerA), which can then convert glyceraldehyde-3-phosphate (G3P) into 3-phosphohydroxypyruvate (3P-HP). Moreover, the modified cyanobacteria of the present invention can also produce phosphoserine transaminase (SerC), which can then convert 3-phosphoserine into phosphoserine (3P-Serine). In addition, the modified cyanobacteria of the present invention can also produce phosphoserine phosphorylase (SerB), which can then convert phosphoserine (3P-Serine) into serine, particularly L-serine.

[0046] Subsequently, since the modified cyanobacteria of the present invention can also produce oxaloacetate decarboxylase, L-serine can be converted into ethanolamine. Further, the ethanolamine oxidase produced by the modified cyanobacteria of the present invention is used to convert ethanolamine into hydroxyacetaldehyde. Finally, the modified cyanobacteria of the present invention can also produce glycolaldehyde reductase to convert hydroxyacetaldehyde into ethylene glycol.

[0047] In an embodiment of the present invention, the culture conditions for converting carbon source into ethylene glycol using modified blue-green bacteria can be as follows: culture at 42°C with a luminescence intensity of 200 μmol / m-2 / s-1, a 12-hour / 12-hour light-dark cycle, an initial NaHCO3 concentration of 25 mM, and a carbon dioxide concentration of 3%. In a cup-flask experiment, 1025 mg / L of ethylene glycol can be produced after 72 hours of culture.

[0048] In this invention, the carbon source can be industrial waste gas, i.e., a mixture of hydrogen, acetylene, methane, hydrogen sulfide, and acetaldehyde. Further, the mixture may contain 30 to 50 ppm of hydrogen, 150 to 250 ppm of acetylene, 100 to 200 ppm of methane, 0.1 to 1 ppm of hydrogen sulfide, and 1 to 5 ppm of acetaldehyde. For example, the industrial waste gas may be a mixture of 40 ppm of hydrogen (H2), 200 ppm of acetylene (C2H2), 150 ppm of methane (CH4), 0.5 ppm of hydrogen sulfide (H2S), and 3 ppm of acetaldehyde (CH3CHO).

[0049] Please see Figure 5 and Figure 6 As shown, Figure 5 The calibration curves were measured for different ethylene glycol concentrations. Figure 6 A graph showing the results of measuring ethylene glycol secreted by cyanobacteria. Figure 5 In the analysis, a refractive index detector was used to detect the ethylene glycol at concentrations of 156, 312, 625, and 1250 mg / mL, respectively. In an embodiment of the invention, the modified cyanobacteria of the present invention were cultured at 37°C and 3% carbon dioxide for 60 hours, and the culture medium was then directly analyzed, yielding the following results: Figure 6 The results are shown. In other words, the modified cyanobacteria of the present invention, after cultivation, can directly detect an ethylene glycol concentration of approximately 1025 mg / mL in the culture medium. In other words, the modified cyanobacteria of the present invention can indeed convert a carbon source into ethylene glycol and directly secrete ethylene glycol outside the strain.

[0050] Furthermore, the modified cyanobacteria of the present invention are suitable for growth in an environment of 30°C to 60°C, enabling their use in treating industrial waste gas. Preferably, the modified cyanobacteria of the present invention are suitable for growth in an environment of 35°C to 50°C. In embodiments of the present invention, with other culture conditions unchanged, the modified cyanobacteria of the present invention were cultured at 37°C and 42°C respectively, and the growth status and ethylene glycol accumulation of the modified cyanobacteria were detected using the optical density of OD730. Figure 7In the diagram, OD37 represents the cell density curve of the modified cyanobacteria at a culture temperature of 37°C; OD42 represents the cell density curve of the modified cyanobacteria at a culture temperature of 42°C; EG37 represents the cumulative amount of ethylene glycol produced as a bar graph at a culture temperature of 37°C; and EG42 represents the cumulative amount of ethylene glycol produced as a bar graph at a culture temperature of 42°C. The growth density of the modified cyanobacteria and the cumulative amount of ethylene glycol increase with culture time. Notably, culturing the modified cyanobacteria at 42°C, compared to culturing them at 37°C, increases the growth rate of the modified cyanobacteria and yields a higher ethylene glycol production. In other words, the modified cyanobacteria of this invention are particularly suitable for growth in an environment of 42°C, which is beneficial for their application in the treatment of industrial waste gas.

[0051] [Beneficial Effects of the Examples]

[0052] One of the beneficial effects of the present invention is that the carbon source to ethylene glycol conversion provided by the present invention can be achieved by using a technical solution of "the plastid including the gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6" and "implanting the plastid into cyanobacteria by electroporation to obtain modified cyanobacteria", thereby utilizing modified cyanobacteria to convert carbon sources into ethylene glycol, achieving the benefits of carbon reduction while obtaining high-economic-value chemicals.

[0053] Furthermore, the present invention improves the success rate of gene transformation by employing a technical solution in which "the gene sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 are located at the first gene locus, and the gene sequences of SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 are located at the second gene locus".

[0054] The content disclosed above is only a preferred and feasible embodiment of the present invention, and is not intended to limit the scope of protection of the claims of the present invention. Therefore, all equivalent technical changes made based on the content of the present invention specification and drawings are included within the scope of protection of the claims of the present invention.

Claims

1. A method for converting a carbon source into ethylene glycol, characterized in that, The method for converting a carbon source into ethylene glycol includes: Provide plasmids, said plasmids comprising gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6; The plastid was implanted into cyanobacteria via electroporation to obtain modified cyanobacteria; and A carbon source is provided to the modified blue-green bacteria, enabling the modified blue-green bacteria to convert the carbon source into ethylene glycol.

2. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The genomic DNA of the cyanobacterium has a first gene locus and a second gene locus, with the gene sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 located at the first gene locus, and the gene sequences of SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 located at the second gene locus.

3. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The modified cyanobacteria have the ability to produce 3-phosphoglycerate dehydrogenase, phosphoserine phosphatase, phosphoserine transaminase, oxaloacetate decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase.

4. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The cyanobacteria mentioned are Synechococcus elongates.

5. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The electroporation process is performed at a voltage of 0.5 to 1.5 kV for 2 to 10 mSec.

6. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The electroporation process further includes adding polyethylene glycol at a concentration of 0.5 to 2 wt%.

7. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The carbon source is carbon dioxide, glucose, sucrose, fructose, or galactose.

8. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The plastid is an Escherichia coli plastid.

9. The method for converting a carbon source into ethylene glycol according to claim 1, characterized in that, The method further includes the step of implanting the plastid into Escherichia coli for mass production.

10. A method for converting a carbon source into ethylene glycol, characterized in that, The method for converting a carbon source into ethylene glycol utilizes modified cyanobacteria to convert the carbon source into serine; wherein the modified cyanobacteria include the gene sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:

6.

11. The method for converting a carbon source into ethylene glycol according to claim 10, characterized in that, The genomic DNA of cyanobacteria has a first gene locus and a second gene locus. SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 are located at the first gene locus, and SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 are located at the second gene locus.

12. The method for converting a carbon source into ethylene glycol according to claim 10, characterized in that, The carbon source is carbon dioxide, glucose, sucrose, fructose, or galactose.

13. The method for converting a carbon source into ethylene glycol according to claim 10, characterized in that, The modified cyanobacteria have the ability to produce oxaloacetate decarboxylase to convert L-serine into ethanolamine.

14. The method for converting a carbon source into ethylene glycol according to claim 13, characterized in that, The modified cyanobacteria have the ability to produce ethanolamine oxidase to convert ethanolamine into hydroxyacetaldehyde.

15. The method for converting a carbon source into ethylene glycol according to claim 14, characterized in that, The modified blue-green bacteria have the ability to produce ethanolaldehyde reductase to convert hydroxyacetaldehyde into ethylene glycol.

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