The invention relates to an extraction method of mouse
platelet. Blood drawn from orbita
vein of mouse is placed into an anticoagulation centrifugal tube, centrifugated at the speed of 800rmp for 10min after standing for 30min, and the extracted supernatant fluid is
plasma rich in
platelet. The supernatant fluid is placed in a clean
test tube and centrifugated at the speed of 3500rpm for 10min. After filtering the supernatant fluid, the sediments at the bottom of the tube are the platelets. 50ul to 100ul of
ammonium oxalate solution with a
mass concentration of 1 percent is added into the
test tube and slightly stirred with a glass rod, and then 2ml to 3ml of
ammonium oxalate solution with a
mass concentration of 1 percent is added, and stood for 5min, to make sure the hematid is dissolved; the hematid is centrifugated at the speed of 3500rpm for 10min, after the supernatant fluid is abandoned, the
test tube is added with a few
platelet scrub solution and blown and beaten to turn the platelet into a platelet suspension. The suspension is centrifugated at the speed of 3300rpm for 10min, removed the supernatant in the suspension, the test tube is added with a few platelet scrub solution and blown and beaten to turn the platelet into a platelet suspension. The platelet is used as an
antigen to immunify guinea pigs, and the serum of such guines pigs is injected into the mouse to prepare
allergic purpura. The preparation method has simple operation, can obtain
high concentration platelet, and can comparatively better solve the problem of impure platelet.