Composition for reparing tissue and regenerating organs by internal in-situ culture and regeneration of stem cells

A tissue repair and organ regeneration technology, applied in the directions of microorganisms, drug combinations, tissue culture, etc., can solve the problems of incomplete restoration of skin physiological structure and function, and no regeneration of human organs.

Inactive Publication Date: 2003-01-29
李俐 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has not been a single case of successful regeneration of a fully functional human organ using this method
For example, treating wounds

Method used

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  • Composition for reparing tissue and regenerating organs by internal in-situ culture and regeneration of stem cells
  • Composition for reparing tissue and regenerating organs by internal in-situ culture and regeneration of stem cells
  • Composition for reparing tissue and regenerating organs by internal in-situ culture and regeneration of stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0412] In the following examples, in vitro experiments demonstrate that the original composition of the present invention has a unique effect on promoting the proliferation and tissue-specific attachment of normally differentiated mammalian and mammalian stem cells, while maintaining the integrity of the skin structure . Skin tissue cells, hair follicle stem cells and skin slices are all sampled from rats or mice and cultured in vitro. Cells or tissues are divided into two groups: the control group is cultured in conventional cell culture medium (complete MEM), and the treatment group is cultured in conventional culture medium added with this original composition. Embodiment 1 mouse skin cell culture

[0413] Immediately after killing the mice, fresh skin was taken and placed in a 6-well Petri dish (approximately 10 4 cells / ml, 7ml / well) in MEM. After three days, normal growth of these cells was observed, and on the eighth day of culture, these cells adhered to the basal l...

Embodiment 2

[0416] Example 2 Culture of Rat Hair Follicle Stem Cells

[0417] Rat hair follicle stem cells were collected from the bulge of the hair follicle immediately after the rat was killed, and cultured in a 24-well MEM / 5% FCS culture dish (2 ml per well). After 5 days, the cells were seen growing well and adhered to the base of the cell culture dish. About 1 g of the composition of the present invention was added to the treatment group, which contained stigmasterol, β-sitosterol, and campesterol dissolved in cottonseed oil at a content of 6% by weight, and 1 ml of MEM was added to the control group. The cultures were observed for 41 days and the microscopic appearance of the cultures was recorded.

[0418] 16A-C show: the results of in vitro experiments on rat hair follicle stem cells in culture medium with or without the composition of the present invention. The right column pictures show the control group, the left column is from the treatment group. As shown in the right co...

Embodiment 3

[0419] These results confirm that the composition of the present invention not only has the ability to promote proliferation, but also promotes tissue-specific adhesion of stem cells. This is consistent with the effects produced by treatment of adult cells with this composition. Example 3 Mouse Skin Tissue Culture

[0420] Immediately after the mice were killed, fresh skin was taken, cut into slices, and cultured in MEM culture dishes and 6 pools of MEM / 15%FCS culture dishes (5ml / pool contains 3 pieces of skin). After 4 days, the flaps were attached to the base of the cell culture dish. About 6 g of the composition of the present invention was added to the treatment group, which contained 1% by weight of α-spinasterol and 0.001% by weight of berberine in soybean oil. In the control group, 6ml of MEM was added. These cultures were observed for 44 days and the microscopic appearance of the cultures was recorded.

[0421] Figures 17A-B show the results of in vitro experiment...

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Abstract

A composition for in-situ culture and regeneration of stem cells in vivo to repair tissue and regenerate organ, especially suitable for those in vitro, is prepared from the oil-soluble sterol compounds (at least 0.01 wt.%).

Description

technical field [0001] The present invention relates to a composition for tissue physiological repair and organ functional regeneration, more specifically, relates to a composition, which can induce in situ pluripotent regenerative stem cells in vivo to proliferate, realize the Or the physiological repair of human tissue and regeneration of fully functional human organs. The invention also relates to methods of use and / or other uses of the composition. Background technique [0002] The tissue engineering industry is accelerating the development of technological advances in the large-scale production of cell cultures and biomaterials, which are produced or synthesized in vitro, that is, outside the body of an animal or human body, and then transplanted into a host to achieve tissue repair or other therapeutic purposes. [0003] One of the methods of modern tissue engineering is to implant a synthetic material into the human body as a structural scaffold to support the growt...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/11A61K31/20A61K31/201A61K31/575A61K35/644A61K36/185A61K36/31A61K36/48A61K36/539A61K36/66A61K36/718A61K36/756A61K36/88A61K36/889A61K36/899A61K45/06
CPCA61K45/06A61K36/48A61K31/201A61K31/20A61K36/539A61K36/31A61K36/88A61K36/185A23V2002/00A23L1/3004A61K36/756A61K36/718A61K36/899A61K31/575A61K36/66A61K36/889A23L33/11
Inventor 徐荣祥
Owner 李俐
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