A method for preparing a *Lactobacillus plantarum* enrichment agent and its application.

By preparing a microbial enrichment agent through enzymatic hydrolysis of Chinese herbal medicine residues, the problems of insufficient utilization of Chinese herbal medicine residues and insufficient viable Lactobacillus plantarum counts have been solved, achieving efficient increase in bacterial count and economical utilization of resources.

CN120400013BActive Publication Date: 2026-05-26JIANGZHONG PHARMA CO LTD
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Patent Information

Application Number
CN202510921056.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-07-04
Publication Date
2026-05-26
Estimated Expiration
2045-07-04

AI Technical Summary

Technical Problem

In existing technologies, the residue generated after the extraction of traditional Chinese medicine cannot be effectively utilized, leading to environmental pollution and resource waste. At the same time, traditional Chinese medicine extraction efficiency is low, the effective components and nutrients are not fully released, and the number of live bacteria in the fermentation broth of Lactobacillus plantarum is insufficient, and there is little research on enrichment agents.

Method used

Using Chinese herbal medicine residue as raw material, a probiotic is prepared through enzymatic hydrolysis. A complex enzyme, including cellulase, pectinase, α-amylase, papain, and snail enzyme, is used to adjust the pH and temperature for enzymatic hydrolysis, resulting in a highly efficient probiotic for *Lactobacillus plantarum*, which is used to increase the viable count of *Lactobacillus plantarum* in the fermentation broth.

Benefits of technology

It significantly increased the number of viable bacteria of *Lactobacillus plantarum* in the fermentation broth, reduced production costs, realized the resource utilization and economic added value of the medicinal residue, and enhanced the effect of fermented products.

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Abstract

This invention discloses a method for preparing a *Lactobacillus plantarum* enrichment agent and its application, relating to the field of microbial technology. The preparation method includes: extracting traditional Chinese medicine to obtain medicinal residue; enzymatically hydrolyzing the residue; adjusting the pH to neutral to obtain the enrichment agent; wherein the traditional Chinese medicine is selected from one or more of the following: dried tangerine peel, white hyacinth bean, coix seed, polygonatum, lotus leaf, dendrobium officinale, licorice, atractylodes macrocephala, poria cocos, chicken gizzard lining, roasted malt, wolfberry, kudzu root, and clove. The *Lactobacillus plantarum* enrichment agent provided by this invention uses medicinal residue as raw material and is specifically processed using a compound enzyme. It has low raw material costs, high product added value, and can significantly increase the viable count of *Lactobacillus plantarum* in a unit volume of fermentation broth, effectively reducing the cost of preparing highly active *Lactobacillus plantarum* inoculants.
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