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Cultivation method of genuine salvia

A breeding method and technology of Danshen, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problem of no authentic Danshen

Active Publication Date: 2014-06-04
陈瑞阳
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Problems solved by technology

However, my country does not have a unified authentic salvia cultivar so far.

Method used

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  • Cultivation method of genuine salvia
  • Cultivation method of genuine salvia
  • Cultivation method of genuine salvia

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Embodiment Construction

[0028] The present invention can be embodied from the following examples, but it does not limit the invention in any way.

[0029] The cultivation method of the green super authentic Salvia miltiorrhiza provided by the invention comprises the following steps:

[0030] ⑴Establish the germplasm resource nursery of Salvia miltiorrhiza in China, and collect germplasm resources of wild salvia miltiorrhiza across the country;

[0031] ⑵Research on germplasm resources of Danshen in my country from the aspects of morphology, cell biology, molecular biology, and chemistry (determination of drug components), and select representative authentic germplasm resources of Danshen.

[0032] (3) To identify the genome chromosome structure of the selected authentic Danshen germplasm resources.

[0033] (4) Using AFLP molecular marker technology (Wang Bing et al.: Chinese Journal of Traditional Chinese Medicine 32 (19) 2007.10)) to determine the authentic Danshen DNA barcode.

[0034] (5) Tripl...

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Abstract

The invention relates to a cultivation method of genuine salvia, which comprises the following steps of: establishment of a salvia germplasm resource garden, selection of representative genuine salvia germplasm resources, identification of genuine salvia genome karyotype structure, determination of genuine salvia DNA bar codes, artificial mutation to obtain autotetraploid, sexual hybridization to obtain triploid salvia permanent hybrids, detoxification treatment, tissue culture seedling cloning and proliferation, bottle seedling transplanting, transplanted seedling management, new species comparison and testing, determination of the medicinal material yield and the main drug effective components, and genuine salvia new species determination and species identification. The genuine salvia obtained in the invention is, so far, the only population that has two great natural advantages: the polyploid advantage and the heterosis, and the advantages are giant and irreplaceable. Therefore, the method of the invention is an effective method for cultivating genuine traditional Chinese medicine with strong biotic stress and abiotic stress capability by using traditional Chinese medicine genuine functional genomes and feature genes and based on inherent genes of genuine traditional Chinese medicine.

Description

technical field [0001] The invention relates to a method for cultivating new varieties of plants, in particular to a method for cultivating green super authentic salvia miltiorrhiza. Background technique [0002] Salvia ( Salvia miltiorrhiza Bunge) is one of the earliest and most widely used traditional Chinese medicines in my country. It is a famous medicine for promoting blood circulation and removing stasis. With the aging population in our country, cardiovascular disease has become a major disease that endangers human health, the incidence rate is increasing year by year, and the demand for drugs is increasing. At present, there are more than 100 kinds of compound Chinese medicines produced with Danshen as raw materials in my country, involving more than 100 enterprises, and there is a huge demand for high-quality Danshen medicinal materials. However, my country does not have a unified authentic salvia cultivar so far. In recent years, in order to meet the third gree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 陈瑞阳
Owner 陈瑞阳
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