Preparation method of chromosome suitable for karyotype analysis of rubus plants

A technology of karyotype analysis and Rubus genus, which is applied in the field of cytogenetics and cell biology, can solve the problem that the effect of chromosome samples is not as good as the technical process of the method, the method of enzymatic hydrolysis to remove the wall and low osmosis is cumbersome, and the cost of making chromosome karyotypes is increased, etc. problem, to achieve the effect of high specimen quality, easy coloring, and low production cost
CN102492767AInactive Publication Date: 2012-06-13SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2012-06-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of chromosome suitable for karyotype analysis of rubus plants. The chromosome preparation method comprises the following specific steps of: material culturing; pretreatment; fixing; storage; dissolution; dyeing, smearing and tabletting; and microscopic examination and observation. The preparation method disclosed by the invention has the advantages of low cost, simple, convenient and speedy operation process and capability of obtaining an ideal chromosome specimen for karyotype analysis of the rubus plants.
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Description

technical field

[0001] The invention belongs to the field of cytogenetics and cell biology, and relates to a chromosome preparation method, in particular to a chromosome preparation method suitable for karyotype analysis of Rubus plants. Background technique

[0002] Chromosome is the basis of genetic material in organisms and the main carrier of genetic material. Chromosome size, number, and shape (ie, karyotype characteristics) are relatively stable during the growth and generation of plants, and are not easily affected by changes in environmental conditions. They can largely reflect the characteristics of species and their genetic differences. Therefore, the karyotype characteristics and even the number of chromosomes of different species can provide cytological basis for the classification, phylogeny and genetic relationship identification of this genus. When conducting comparative studies on the number, shape, and karyotype of chromosomes in a specific group of cells, ...

Claims

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