Identification method research of wheat-secale cereale L 1BL/1RS translocation line
An identification method and a technology of translocation lines, applied in the field of cell biology, can solve the problems of difficulty in breeding new varieties, poor quality of wheat flour baking and processing, etc.
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[0061] 1. Cytogenetic identification method of 1BL / 1RS translocation line
[0062] (1) Chromosome analysis of mitosis and meiosis
[0063] Satellites or secondary constrictions on 1RS do not appear in the wheat background. There are usually four chromosomes (1B, 6B) in metaphase I cells with obvious satellites on the short arms of common wheat. If 1RS replaces 1BS, 1BL / There are only two chromosomes in the 1RS translocation line with satellites, which can quickly determine the existence of 1RS, but it is only applicable to the situation where 1RS replaces 1BS, and it is not applicable when replacing 1AS or 1DS (William, Euphytica, 1998 , 108:1-19). Mettin, Zeller and Berzonsky identified the 1BL / 1RS translocation line by counting the chromosomes containing satellites in metaphase cells of mitosis.
[0064] In addition, using chromosomal homology, use the material to be tested to cross the 1B double monomer or 1B / 1R translocation line of common wheat, and judge whether it is...
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