Identification method research of wheat-secale cereale L 1BL/1RS translocation line

An identification method and a technology of translocation lines, applied in the field of cell biology, can solve the problems of difficulty in breeding new varieties, poor quality of wheat flour baking and processing, etc.

Inactive Publication Date: 2010-06-30
李祥
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although it is generally believed that there is a Sec-1 locus encoding ryeine on 1RS, the replacement of 1BS by 1RS has caused the loss of important gene loci Glu-3 and Gli-1 on 1BS, resulting in the baking of 1BL/1RS

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Identification method research of wheat-secale cereale L 1BL/1RS translocation line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0061] 1. Cytogenetic identification method of 1BL / 1RS translocation line

[0062] (1) Chromosome analysis of mitosis and meiosis

[0063] Satellites or secondary constrictions on 1RS do not appear in the wheat background. There are usually four chromosomes (1B, 6B) in metaphase I cells with obvious satellites on the short arms of common wheat. If 1RS replaces 1BS, 1BL / There are only two chromosomes in the 1RS translocation line with satellites, which can quickly determine the existence of 1RS, but it is only applicable to the situation where 1RS replaces 1BS, and it is not applicable when replacing 1AS or 1DS (William, Euphytica, 1998 , 108:1-19). Mettin, Zeller and Berzonsky identified the 1BL / 1RS translocation line by counting the chromosomes containing satellites in metaphase cells of mitosis.

[0064] In addition, using chromosomal homology, use the material to be tested to cross the 1B double monomer or 1B / 1R translocation line of common wheat, and judge whether it is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Secale cereale L is one of the important kindred plants of wheat and has the characteristics of floweriness, multi-powder, eurytopicity, and the like. The 1RS segment of secale cereale L carries resistance genes Lr26, Sr31, Yr9 and Pro8 for resisting leaf rust, stem rust, stripe rust and powdery mildew as well as plant hopper resistant gene Gb2 and a great amount of anti-retrieval genes. The content of soluble fiber of 1BL/1RS translocation wheat flour is higher than that of ordinary wheat and is beneficial to human body. Related genes of a plurality of excellent traits for increasing the QTL loci, improving the yield traits and increasing the protein content exists on the 1RS. Me1z, Schlegel and Thiele find that partial isogeny relationship exists between 1RS, 1RL, 2RL, 3RS and 5RS and corresponding wheat chromosome arms via ph16 line analysis of addition line, telomere line and the wheat, thereby being capable of producing abnormal addition line, replacement line and translocation line of wheat-secale cereale L. As 1RS can favorably compensate negative effect caused by 1BS defect on the heredity, the wheat 1B/1R translocation line can be directly applied to wheat breeding. The researched is carried out based thereon.

Description

technical field [0001] The invention relates to the research on the identification method of the wheat-rye 1BL / 1RS translocation line, which belongs to the field of cell biology. Background technique [0002] Rye (Secale cereale L) is one of the important relatives of wheat, which has the characteristics of many flowers, many powders, and wide adaptability (Wu Jinhua, Journal of Wheat Crops, 2005, 25(1): 115-119). The rye 1RS fragment carries the resistance genes Lr26, Sr31, Yr9, Pro8 for leaf rust, stem rust, stripe rust and powdery mildew resistance, planthopper resistance gene Gb2 and a large number of stress resistance genes, 1BL / 1RS translocation wheat flour The soluble fiber content of wheat is higher than that of general wheat, which is beneficial to the human body (Shang Haiying, Southwest Agricultural Journal, 2004, 17(3): 273-277). There are QTL loci for increasing the number of spikelets and genes related to various excellent traits such as increasing yield trait...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68G01N33/577G01N33/561G01N30/02
Inventor 李祥
Owner 李祥
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap