30 results about "Repetitive Regions" patented technology

The present invention provides for compositions and methods for amplifying target nucleic acids using nucleic acid primers designed to limit non-target nucleic acid dependent priming events. The present invention permits amplifying and quantitating the number of repetitive units in a repetitive region, such as the number of telomere repetitive units.

A method, system aid a computer program product for evaluating a object; the method includes: (i) obtaining an image of an area of the object; wherein the area comprises multiple arrays of repetitive structural elements that are at least partially surrounded by at least one group of non-repetitive regions; wherein non-repetitive regions that belong to a single group of non-repetitive regions are ideally identical to each other; wherein the non-repetitive regions are arranged in a repetitive manner; and (ii) providing an evaluation result in response to a comparison between image information of a first sub-area to image information of a second sub-area that is proximate to the first sub-area; wherein the first sub-area comprises a first array of repetitive structural elements and a first non-repetitive region; wherein the second sub-area comprises a second array of repetitive structural elements and a second non-repetitive region.

The present invention provides for compositions and methods for amplifying target nucleic acids using nucleic acid primers designed to limit non-target nucleic acid dependent priming events. The present invention permits amplifying and quantitating the number of repetitive units in a repetitive region, such as the number of telomere repetitive units.

The invention provides a primer composition for lysosomal disease gene screening and a kit using the same. The primer composition comprises multiple primer sequences respectively aiming at non-repetitive regions of IDUA, ARSB, MAN2B1, ST3GAL5, SMPD1, CTNS, MFSD8, IDS, GUSB, GBA, ARSA, GNPTAB, SLC17A5, CLN8, SGSH, HYAL1, PSAP, GNPTAG, PPT1, CTSD, NAGLU, FUCA1, GLB1, SUMF1, MCOLN1, TPP1, HGSNAT, NAGA, GM2A, GALC, LIPA, CLN3, GALNS, NEU1, HEXA, GLA, NPC1, CLN5, MANBA, HEXB, ASAH1, NPC2 and CLN6 genes.

A method, system and a computer program product for evaluating a object; the method includes: (i) obtaining an image of an area of the object; wherein the area comprises multiple arrays of repetitive structural elements that are at least partially surrounded by at least one group of non-repetitive regions; wherein non-repetitive regions that belong to a single group of non-repetitive regions are ideally identical to each other; wherein the non-repetitive regions are arranged in a repetitive manner; and (ii) providing an evaluation result in response to a comparison between image information of a first sub-area to image information of a second sub-area that is proximate to the first sub-area; wherein the first sub-area comprises a first array of repetitive structural elements and a first non-repetitive region; wherein the second sub-area comprises a second array of repetitive structural elements and a second non-repetitive region.

The invention provides a method and device for identifying repetitive regions in deoxyribonucleic acid (DNA) sequences. The method comprises the steps of: identifying occurrence number of constructedn-item sequences in the DNA sequences; taking the n-item sequences with the occurrence number greater than a preset threshold as the repetitive regions and constructing a n-item sequence set of all the n-item sequences serving as the repetitive regions; and if the number of the n-item sequences in the n-item sequence set is not unique, constructing (n+1)-item sequences between two n-item sequencesin the n-item sequence set according to a preset rule. Compared with the prior art, the method provided by the embodiment of the invention has the advantages that only the constructed DNA subsequences are needed to be identified, so that identified objects are greatly reduced; the process of obtaining the repetitive regions can also be obtained by counting the occurrence number in the identification process, so that the identifying efficiency is further improved; and longer DNA subsequences are constructed from the repetitive regions through the preset rule with no need for firstly combiningthe repetitive regions with single bases and traversing the entire DNA sequence one by one, so that the identifying efficiency of the genomic repetitive regions can be greatly improved.

The invention relates to the field of information analysis of sequencing data, and particularly provides a method for detecting and typing the repetition number of a short tandem repeat sequence based on next-generation sequencing. According to the detection method, two flanking sequences can be compared at the same time across a middle repeated region, and mismatching, insertion and deletion of the flanking sequences are considered at the same time; the typing method is an STR typing method for performing dynamic threshold setting by combining repetition number difference based on detection of an effective peak value, and can be applied to detection of repetition numbers of different next-generation sequencing platforms.

The invention relates to the technical field of wheat functional gene localization, in particular to a probe design method for wheat exon sequencing gene localization, which comprises the following steps of: sequencing through a high-throughput sequencing platform to obtain transcriptome data, comparing and analyzing the transcriptome data with a comparison reference genome, splicing and combining transcripts, and performing ORF prediction; and deleting the sequence of the repeated region, and performing high-density probe synthesis on the screened sequence to obtain the probe. The gene localization method comprises the following steps: breaking wheat DNA into fragments, hybridizing the broken wheat DNA with the high-density probe, enriching magnetic beads, eluting the liquid, and carrying out high-throughput sequencing, variation detection and statistical localization. The invention solves the problem of high gene positioning cost caused by huge wheat genome in the prior art, and by designing the superhigh-density multiple primer probe, sequencing is only carried out on the gene exon sequence, and the sequencing cost is reduced by 80% under the condition of the same gene sequencing depth.

Disclosed is a method whereby a repetitive nucleic acid sequence, such as a short tandem repeat (STR), may be characterized as to its length. Pyrosequencing is used to sequence an STR repetitive region to measure the length of STRs in a rapid manner. A combinatorial approach is disclosed for the addition of multiple nucleotides (e.g., two mononucleotides) at a time by the polymerase, which reduces the sample analysis time by half. In addition, modified nucleic acids, such as peptide nucleic acids, are used as blocking probe to stop polymerization on the flanking region which makes it possible to use pyrosequencing for DNA length measurement both in the case of homozygous or heterozygous samples for varying repeat patterns of different markers. Further, dideoxynucleotides are added to stop polymerization in the flanking region of the STR.

The invention provides a method, a system and a computer program product for evaluating objects. The method for evaluating objects comprises the following steps: (1) obtaining the image of a region ofan object, wherein the region comprises repetitive structure elements of a plurality of arrays which are at least partly surrounded by at least a group of non-repetitive regions, the non-repetitive r egions belonging to the single group of non-repetitive region are ideally identical, and the non-repetitive regions are arranged in a repetitive mode; (2) and responding to the comparison between the image information of a first sub-region and the image information of a second sub-region close to the first sub-region to provide an evaluation result, wherein the first sub-region comprises repetitive structure elements of a first array and a first non-repetitive region, and the second sub-region comprises repetitive structure elements of a second array and a second non-repetitive region.

Three genes encoding secretory membrane proteins have been successfully isolated from an osteoblast-like cell line by a method for specifically cloning secretory membrane proteins. One of these genes encodes a novel receptor protein. The protein has only the extracellular region, binds to the cell membrane via a GPI anchor, and carries therein a cysteine-rich, repetitive region commonly conserved in the TNF receptor super family. Overexpression of this protein in osteoblast-like cell line cells suppresses cell proliferation, changes cell morphology, and increases alkaline phosphatase activity, which is one of markers for differentiation of osteoblasts.

The invention relates to the field of information analysis of sequencing data, and particularly provides a method for detecting and typing the repetition number of a short tandem repeat sequence based on next-generation sequencing. According to the detection method, two flanking sequences can be compared at the same time across a middle repeated region, and mismatching, insertion and deletion of the flanking sequences are considered at the same time; the typing method is an STR typing method for performing dynamic threshold setting by combining repetition number difference based on detection of an effective peak value, and can be applied to detection of repetition numbers of different next-generation sequencing platforms.

Methods of sequencing and assembling a nucleic acid sequence from a nucleic acid sample containing repetitive or low-information regions, which are typically difficult to sequence and / or assemble are provided. The methods of sequencing and assembling introduce mutations into the sample to increase sequence diversity between various repetitive regions present in the nucleic acid sample. This sequence diversity allows various segments to assemble independently of different, but similar sequences present in the nucleic acid sample.

The invention relates to a method for predicting the copy number of a gene to be tested, a computing device and a storage medium. The method includes: obtaining sequencing sequence data of the sample to be tested, the sequencing sequence data is a third-generation sequencing sequence of the gene to be tested obtained through the third-generation sequencing technology; based on the obtained sequencing sequence data, determining a single sequence of the gene to be tested The number of haplotypes and the sequencing depth; and based on the determined number of haplotypes and the sequencing depth, determining a prediction result about the copy number of the gene to be tested in the sample to be tested. The invention can accurately determine the gene copy number for repeated regions or highly homologous genes at low cost.