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Molecular marker method for avirulence gene of rice blast

A technology of rice blast fungus and non-toxic genes, applied in the direction of DNA/RNA fragments, recombinant DNA technology, fermentation, etc., can solve the problems of complex operation procedures, inconvenient analysis of non-toxic genotoxic components, etc., to save time and cost, molecular Marker Stabilization, Marker Stabilization Effects

Inactive Publication Date: 2007-04-25
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Since this type of marker requires complicated procedures, expensive biochemical reagents, and DNA from a large number of samples, it is not convenient for the analysis of toxic components of non-toxic genes

Method used

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  • Molecular marker method for avirulence gene of rice blast
  • Molecular marker method for avirulence gene of rice blast
  • Molecular marker method for avirulence gene of rice blast

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Embodiment Construction

[0027] The molecular marker method for the linkage of Avr-Pit, Avr-Pia and PRE1 of the rice blast fungus avirulent gene, including:

[0028] 1) Using two sets of parents Guy11 and JS153, CH87 and 6023, the parent strains are known and public (Chen Qinghe, Wang Yuanchao, Zheng Xiaobo. Differences in mating type distribution and fertility ability of blast fungus in major rice regions in China, Chinese Agricultural Sciences 2004, 37(6): 840-845) provided by the Key Laboratory of Disease and Pest Monitoring and Control, Ministry of Agriculture, to obtain sexual offspring by hybridization, and use the parent and offspring strains to identify rice varieties Aichi-asahi, K59 and Lijiang Xintuan Heigu ( Aichi-asahi, K59 and Lijiang Xintuan Heigu varieties are all publicly known and public, and provided by the Key Laboratory of Disease and Pest Monitoring and Control of the Ministry of Agriculture. For the pathogenicity determination, methods and varieties, please refer to the reference...

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Abstract

The invention of molecular labeling method of Pyricularia grisea avirulent gene belongs to the field of agricultural biotechnology. The molecular labeling technology includes performing molecular labeling to avirulent gene though assessment from two parental strains and filial generation groups of Pyricularia grisea. M355-356 labeled by SSR near NO. 1 chromosome telomere links with avirulent gene Avr-Pit with a genetic distance of 2.3cm; S487 labeled by RAPD located near NO. 7 chromosome telomere links with avirulent gene Avr-Pia with a genetic distance of 3.5cm; S361 labeled by m677-678 and RAPD located at middle of N0. 3 chromosome telomere links with Pyricularia grisea specified avirulent gene PRE1 with genetic distances of 5.9cm and 6.4. The invention can not only make use of the nontoxic gene marker as gene probe, but also set foundations for further clone of the gene through physical mapping.

Description

(1) Technical field [0001] This research has invented the linkage molecular markers and chromosomal location of rice variety-specific avirulence genes Avr-Pit, Avr-Pia and host species-specific avirulence gene PRE1 of Magnaporthe grisea, which belongs to the field of biotechnology. It is specially used for the monitoring of the toxicity composition and evolution rule of Magnaporthe grisea in the field, and it can also be used for the cloning of the avirulent gene of Magnaporthe grisea. (2) Technical background [0002] Rice is one of the important food crops, and rice blast is one of the most widely distributed and most serious rice diseases in the world, and has become a major obstacle to high and stable rice yields (Ou S H.1980, Pathogen variability and host resistance in rice blast disease.Ann Rev Phytopathol.18:167-187.), the harm it causes is seriously threatening human food security. The control of rice blast is the same as other rice diseases, through the use of fung...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12P19/34
Inventor 郑小波王源超陈庆河张正光
Owner NANJING AGRICULTURAL UNIVERSITY
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