The invention discloses a hair conditioner and a preparation method of the hair conditioner. The hair conditioner is prepared from the following raw materials: 1-5 parts of glycerinum, 1-3 parts of 1,3-propylene glycol, 2-4 parts of honey, 1-5 parts of c dimethyl silicone polymer etostearyl alcohol, 5-15 parts of N-sodium lauroyl sarcosine, 5-15 parts of hydrolyzed sericin, 0.2-2 parts of EDTA disodium, 0.3-3 parts of citric acid, 0.1-1 part of dimethyl silicone polymer, 0.01-0.1 part of essence, 5-15 parts of a cationic surfactant, 0.02-0.2 part of a bacteriostatic agent, and 55-75 parts of water. The hair conditioner is convenient and safe to use, the cost of the raw materials is low, the conditions of hair damages like coarse hair, splitting and breakage, caused by misuse of shampoo products, hair dyeing, perming, ultraviolet irradiation, malnutrition and the like, are remarkably inhibited, the hair root is nourished, nutrients and water is supplemented for the hair, the hair becomes smooth and elastic, and no skin allergic reaction is caused after the hair conditioner is used for a long period of time.