Moisturizing and soothing composition and application thereof

Abstract

The invention relates to the technical field of daily chemical industry, and discloses a moisturizing and soothing composition and application thereof.The moisturizing and soothing composition comprises, by weight, 20-25% of C14-16 sodium olefin sulfonate, 20-25% of C14-16 sodium alkyl sulfonate, 20-25% of C14-16 sodium alkyl sulfonate, 10-20% of C14-16 0.3%-0.7% of a cationic polymer; a litchi pericarp extract; an aloe extract; and a rosemary extract; wherein the total weight percentage of the litchi peel extract, the aloe extract and the rosemary extract is 1.9%-3.3%. The components are compounded, the cationic polymer and the anionic surfactant are utilized to construct a deposition system, and the three plant extracts with specific total content are cooperated, so that the retention rate of active ingredients in scalp is effectively increased, and the problem that moisturizing and soothing ingredients are easy to wash away is solved. The invention also provides a preparation method combining hot preparation and cold preparation, and the stability of the system and the activity of the extract are ensured by homogenizing the phase A at high temperature and adding the phase E at low temperature. The composition can be used for preparing shampoo products for moisturizing or relieving scalp.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of daily chemical industry, in particular to a moisturizing and soothing composition and application thereof. BACKGROUND

[0002] The scalp health relies on the natural moisturizing film formed by sebaceous glands and sweat glands to maintain the water-oil balance. However, the external environmental stimulation, chemical residues or excessive cleaning often destroy this balance, leading to impaired scalp barrier function, and then dryness, tightness, itching or redness and other discomfort symptoms. Therefore, it is particularly important to develop products that can maintain the scalp ecological environment and have long-lasting moisturizing and soothing functions.

[0003] In order to improve these problems, the existing technology often adds active ingredients with moisturizing or soothing effects to cleaning products (such as shampoos). For example, litchi peel extract, aloe extract and rosemary extract have been proved to have certain antioxidant, anti-inflammatory or moisturizing effects.

[0004] However, in the rinse-off products such as shampoos, the contact time of these active ingredients with the scalp is short, and most of them are lost with water during rinsing. This makes it difficult for active substances to be effectively deposited on the scalp, and they cannot continue to play a role after rinsing, so the actual effect of their long-lasting moisturizing and soothing is greatly limited.

[0005] Therefore, the present application provides a moisturizing and soothing composition and application thereof to solve the problems of the prior art. SUMMARY

[0006] In view of the deficiencies of the prior art, the present application provides a moisturizing and soothing composition and application thereof, which solves the problem that the cleaning composition in the prior art, especially the composition based on sodium olefin sulfonate, although has strong cleaning power, often causes dryness, tightness or irritation of the scalp due to excessive cleaning.

[0007] To solve the above problems, the present application provides the following technical solutions: In a first aspect, the present application provides a moisturizing and soothing composition, which adopts the following technical solutions: A moisturizing and soothing composition, comprising, in percentage by weight: sodium C14-16 olefin sulfonate, content of 20% to 25%; cationic polymer, content of 0.3% to 0.7%; litchi peel extract; aloe extract; rosemary extract; wherein the total weight percentage of the litchi peel extract, the aloe extract and the rosemary extract is 1.9% to 3.3%.

[0008] By adopting the above technical solutions, the composition of the present application promotes the effective deposition and synergistic effect of the active ingredients by specific component compounding while cleaning the scalp. The technical principle is that: Synergistic effect of efficacy components: The present invention found that when the extract of litchi pericarp, the extract of aloe vera and the extract of rosemary are compounded at a total concentration of 1.9% to 3.3%, the three extracts show synergistic effect in moisturizing and soothing scalp, and the effect is better than that of single component or combination with total amount less than 1.9%.

[0009] Construction of deposition system: The sodium C14-16 olefin sulfonate in the composition as an anionic primary surfactant provides basic cleaning power. The cationic polymer as a deposition aid, together with the anionic sodium C14-16 olefin sulfonate and the auxiliary surfactant added in the subsequent preferred scheme, forms polymer surfactant complex micelles or flocs through electrostatic interaction when diluted with water.

[0010] Encapsulation and release of efficacy components: During the formation of the above flocs, the extract of litchi pericarp, the extract of aloe vera and the extract of rosemary as efficacy active substances are effectively encapsulated in the flocs. During the shampooing process, the flocs are preferentially deposited on the scalp and hair surface, so that the active ingredients can be retained after rinsing, thereby exerting their moisturizing and soothing effects.

[0011] Preferably, the composition comprises: the extract of litchi pericarp at a content of 1%; the extract of aloe vera at a content of 0.8%; and the extract of rosemary at a content of 1%.

[0012] Preferably, the cationic polymer comprises polyquaternium-10.

[0013] Preferably, the cationic polymer is polyquaternium-10 at a content of 0.3% to 0.5%.

[0014] Preferably, the composition further comprises at least one auxiliary surfactant selected from cocamide MEA, cocamidopropyl hydroxysultaine, cocamidopropyl betaine, sodium lauroyl glutamate and sodium lauroyl methyl bisalaninate. Through the compounding of the above auxiliary surfactants, the mildness, foam performance and system stability of the composition can be further adjusted.

[0015] Preferably, the composition further comprises piroctone olamine at a content of 0.25%. The addition of piroctone olamine enables the composition to have the effect of dandruff removal while moisturizing and soothing.

[0016] In a preferred embodiment, the composition consists of the following components in percentage by weight: sodium C14-16 olefin sulfonate: 23%; cocamidopropyl hydroxysultaine: 11%; sodium lauroyl methyl amino acid: 8%; cocamidopropyl betaine: 4.4%; sodium lauroyl glutamate: 4%; cocamide MEA: 2.5%; polyquaternium-10: 0.3%-0.5%; guar hydroxypropyltrimonium chloride: 0%-0.2%; piroctone olamine: 0.25%; PPG-3 octyl ether: 0.5%; polyquaternium-7: 0.5%; lychee peel extract: 0.3%-1.2%; aloe extract: 0.4%-0.9%; rosemary extract: 0.5%-1.2%; glycerin: 1%; citric acid: 0.4%; disodium EDTA: 0.1%; phenoxyethanol: 0.5%; sodium benzoate: 0.4%; fragrance: 0.8%; menthol: 0.1%; and the balance of water. This scheme defines the specific amount of each component, and provides a formula embodiment with excellent comprehensive performance.

[0017] In a second aspect, the present application provides a preparation method of a moisturizing and soothing composition, which adopts the following technical scheme: A method for preparing a moisturizing and soothing composition, comprising the following steps: (S1) mixing A-phase raw materials, wherein the A-phase raw materials comprise water, sodium C14-16 olefin sulfonate, a cationic polymer, and at least one auxiliary surfactant, heating to 80-85°C and homogenizing at a rotation speed of 2000-2800 rpm to obtain an A-phase mixture; (S2) cooling the A-phase mixture to 42-45°C; (S3) adding E-phase raw materials comprising lychee peel extract, aloe extract, and rosemary extract to the cooled A-phase mixture and stirring uniformly to obtain the moisturizing and soothing composition.

[0018] By adopting the above technical scheme, the present application provides a preparation process combining hot preparation and cold preparation, to solve the technical problem of poor thermal stability of different components.

[0019] The technical principle of the method is that in step (S1), the A-phase raw materials (especially cationic polymer and surfactant system) must be homogenized at a high temperature of 80-85 DEG C; this heat treatment step is necessary for the full hydration, swelling and dispersion of the cationic polymer in the water phase and the surfactant complex system. Only through sufficient heat homogenization, a stable and uniform A-phase mixture can be built, laying a foundation for the formation of the subsequent efficacy ingredient deposition system. In step (S3), the E-phase raw materials (litchi peel extract, aloe extract and rosemary extract) are heat-sensitive efficacy ingredients; if they are added at the high temperature in step (S1), the bioactive ingredients will be degraded or inactivated, resulting in reduced moisturizing and soothing efficacy of the final product. Therefore, the application sets a forced cooling step in step (S2) to reduce the system temperature to a safe range of 42-45 DEG C. The addition of the E-phase raw materials at this low temperature can completely retain the activity of the plant extracts.

[0020] Preferably, the heating temperature of the A-phase mixture is 80 DEG C, the homogenization speed is 2000 rpm; and the cooling temperature of the A-phase mixture is 45 DEG C. By adopting the above technical scheme, specific process parameters are provided. 80 DEG C is an effective temperature for realizing the dissolution of the cationic polymer, and the homogenization speed of 2000 rpm can effectively disperse the materials. The addition of the E-phase at a temperature of 45 DEG C provides a safe temperature window for the addition of heat-sensitive active substances, and is an optimal process condition for realizing the efficacy and stability of the product.

[0021] In a third aspect, the application provides the use of a moisturizing and soothing composition, and the use of the composition in preparing a shampoo product for moisturizing or soothing the scalp.

[0022] The application provides a moisturizing and soothing composition and its use. The application has the following beneficial effects: 1. The total content of the three active ingredients of litchi peel extract, aloe extract and rosemary extract is limited in a specific synergistic effect range; the composition in the above ratio shows a synergistic effect better than single component or conventional dosage in moisturizing and soothing; at the same time, the deposition system composed of anionic main surfactant and cationic polymer in the composition can effectively encapsulate the extracts, so that they can form effective deposition on the scalp surface during the washing and rinsing process. This technical feature helps to solve the problem of easy loss of active ingredients in existing shampoo products, and is conducive to realizing long-acting moisturizing and soothing effect.

[0023] 2、The present application adopts specific anionic primary surfactant as the main cleaning ingredient, which ensures the cleaning performance of the product. At the same time, through the compounding of cationic polymer and various auxiliary surfactants, the irritation of the cleaning system is effectively adjusted. This compounding system helps to maintain the balance of the scalp ecological environment and improve the dryness and discomfort of the scalp caused by excessive cleaning. For the scalp sensitive population, the composition shows good mildness, which expands the application range of the product.

[0024] 3、The present application adopts the temperature control preparation method of hot A phase and cold E phase; homogenization at a specific high temperature ensures the sufficient hydration and dispersion of the cationic polymer, and improves the stability of the deposition system. Subsequently, the E phase is added after cooling to a specific low temperature range, which effectively avoids the degradation or inactivation of the bioactive ingredients in the plant extract due to high temperature. This process helps to ensure the physical stability of the final product and the activity of the functional ingredients, and is beneficial to improve the batch consistency of the product. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The hair strand moisture retention rate diagram of the present application. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be described in detail below with reference to the drawings in the specification of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0027] The sources and specifications of the main raw materials and reagents used in the following examples and comparative examples are as follows, and the reagents not specifically explained (such as water, glycerol, citric acid, disodium EDTA, phenoxyethanol, sodium benzoate, fragrance) are all conventional products on the market.

[0028] Polyquaternium-10, CAS No. 68610-92-4, is a cationic hydroxyethyl cellulose, commercially available, with viscosity specification (1% aqueous solution, 25°C) of 300-500 mPa-s. Cocamidopropyl MEA, CAS No. 371967-96-3, is commercially available. Cocamidopropyl hydroxysultaine, CAS No. 68139-30-0, is commercially available. Cocamidopropyl betaine, CAS No. 61789-40-0, is commercially available. Sodium lauroyl glutamate, CAS No. 29923-31-7, is commercially available. Sodium lauroyl sarcosinate, CAS No. 137-16-6, is commercially available. Sodium C14-16 olefin sulfonate, CAS No. 68439-57-6, is commercially available. Piroctone olamine, CAS No. 68890-66-4, is commercially available. PPG-3 octyl ether, CAS No. 29117-02-0, is commercially available. Polyquaternium-7, CAS No. 26590-05-6, is a cationic copolymer of acrylamide and dimethyldiallylammonium chloride, the Polyquaternium-7 used in the present application is commercially available as an aqueous solution with solid content of 8-10%. Menthol, CAS No. 89-78-1 (L-menthol), is commercially available. Litchi peel extract, the commercially available product used in the examples of the present application is a water-soluble extract, with appearance of red-brown to brownish liquid or powder, containing polyphenol ingredients. Aloe extract, the commercially available product used in the examples of the present application is aloe vera leaf juice extract or gel juice, containing aloe polysaccharide and other ingredients. Rosemary extract, the commercially available product used in the examples of the present application is rosemary leaf extract, containing rosemary acid, salvia acid and other ingredients. Dipotassium glycyrrhizinate, CAS No. 68797-35-3, is commercially available. Allantoin, CAS No. 97-59-6, is commercially available. Ginger root extract, is a commercially available ginger root extract. Polyquaternium-39, CAS No. 25136-75-8, is a terpolymer of acrylic acid, diallyldimethylammonium chloride and acrylamide, the Polyquaternium-39 used in the present application is commercially available. Guar hydroxypropyltrimonium chloride, CAS No. 65497-29-2, is a cationic derivative of guar gum, the guar hydroxypropyltrimonium chloride used in the present application is commercially available.

[0029] Examples 1-14: Preparation of moisturizing and soothing compositions Table 1: Formulation composition of Examples 1-8 (% by mass) ; Table 2: Formulation composition of Examples 9-14 (% by mass) ; The following examples 15-17 show the specific steps for preparing any of the compositions in Tables 1 and 2 using different process parameters.

[0030] Example 15: This example provides a method for preparing a moisturizing soothing composition (such as Example 5), comprising the following steps: 1. Clean and sterilize the production equipment and utensils used and reserve for use; 2. Put the raw materials (water, polyquaternium-10, cocamide MEA, cocamidopropyl hydroxysultaine, cocamidopropyl betaine, sodium lauroyl glutamate, sodium lauroyl methyl glutamate, sodium C14-16 olefin sulfonate, glycerin) of Example 5 in Table 1 into the main pot, stir and heat to 80°C, start the homogenizer, control the homogenizer speed at 2000 rpm, disperse and dissolve, keep stirring for 30 minutes, and start cooling.

[0031] 3. Cool to 45°C, add B phase, C phase, pre-dissolved D phase, and E phase (litchi peel extract, aloe vera extract, rosemary extract) which have been mixed and dispersed uniformly, stir evenly, take samples for testing, filter the product with 200 mesh filter cloth after passing the test, and obtain the product.

[0032] Example 16: This example provides a method for preparing a moisturizing soothing composition (such as Example 9), comprising the following steps: 1. Clean and sterilize the production equipment and utensils used and reserve for use; 2. Put the raw materials (water, polyquaternium-10, guar hydroxypropyltrimonium chloride, cocamide MEA, cocamidopropyl hydroxysultaine, cocamidopropyl betaine, sodium lauroyl glutamate, sodium lauroyl methyl glutamate, sodium C14-16 olefin sulfonate, glycerin) of Example 9 in Table 2 into the main pot, stir and heat to 82°C, start the homogenizer, control the homogenizer speed at 2500 rpm, disperse and dissolve, keep stirring for 30 minutes, and start cooling.

[0033] 3. Cool to 43°C, add B phase, C phase, pre-dissolved D phase, and E phase (litchi peel extract, aloe vera extract, rosemary extract) which have been mixed and dispersed uniformly, stir evenly, take samples for testing, filter the product with 200 mesh filter cloth after passing the test, and obtain the product.

[0034] Example 17: This example provides a method for preparing a moisturizing soothing composition (such as Example 14), comprising the following steps: 1. Clean and sterilize the production equipment and utensils used and reserve for use; 2. Put each raw material of phase A of example 14 in table 2 (water, polyquaternium-10, cocamide MEA, cocamidopropyl hydroxysultaine, cocamidopropyl betaine, sodium lauroyl glutamate, sodium lauroyl methylglutamate, sodium C14-16 olefin sulfonate, glycerin) into the main kettle, stir to heat to 85℃, start the homogenizer, control the homogenizer speed at 2800rpm, disperse and dissolve, keep stirring for 30 minutes, start to cool down.

[0035] 3. Cool down to 42℃, add phase B, C, pre-dissolved phase D, phase E (litchi peel extract, aloe extract, rosemary extract) in sequence, stir well, take sample for testing, pass the filter cloth of 200 mesh to filter the product, and get the product.

[0036] The preparation of comparative examples 1-11 all adopt the same process steps as example 15 (i.e. heating at 80℃, homogenizing at 2000rpm, adding at 45℃), and the base formula (phase A, B, C, D) is the same as example 5, the difference is the composition of phase E (active phase), or the key component of phase A.

[0037] Comparative example 1: compared with example 5, the difference is that phase E does not contain litchi peel extract, aloe extract and rosemary extract, but contains 0.4% of potassium glycyrrhizinate.

[0038] Comparative example 2: compared with example 5, the difference is that the component of phase E is replaced by 0.4% of allantoin.

[0039] Comparative example 3: compared with example 5, the difference is that the component of phase E is replaced by 2% of ginger root extract.

[0040] Comparative example 4: compared with example 5, the difference is that the litchi peel extract is not contained in the component of phase E, and 0.2% of potassium glycyrrhizinate is additionally added.

[0041] Comparative example 5: compared with example 5, the difference is that the aloe extract is not contained in the component of phase E, and 0.2% of potassium glycyrrhizinate is additionally added.

[0042] Comparative example 6: compared with example 5, the difference is that the rosemary extract is not contained in the component of phase E, and 0.2% of potassium glycyrrhizinate is additionally added.

[0043] Comparative example 7: compared with example 5, the difference is that polyquaternium-10 is not contained in the component of phase A.

[0044] Comparative example 8: compared with example 5, the difference is that phase E (i.e. litchi peel extract, aloe extract and rosemary extract) is not added.

[0045] Comparative Example 9: Compared with Example 5, the difference is that the E phase component contains only litchi peel extract, and its addition amount is adjusted to 2.8% (i.e., the total mass fraction of the three extracts in Example 5).

[0046] Comparative Example 10: Compared with Example 5, the difference is that the E phase component contains only aloe vera extract, and its addition amount is adjusted to 2.8%.

[0047] Comparative Example 11: Compared with Example 5, the difference is that the E phase component contains only rosemary extract, and its addition amount is adjusted to 2.8%.

[0048] Test 1: Determination of Active Substance Deposition on the Scalp The experimental steps are as follows: (1) Select fresh pig skin, remove subcutaneous fat and connective tissue, remove surface pig hair and cut into 4cm×4cm square samples.

[0049] (2) Use phosphate buffered saline (PBS, pH 5.5) to clean the sample surface to remove dirt and free debris.

[0050] (3) Fix the pigskin sample onto the simulation device (22.0℃, 55%RH); the measurement area is defined as a center 3cm × 3cm (9cm). 2 ).

[0051] (4) Apply 0.5 mL of the sample to be tested to the test area, add 0.5 mL of warm water (about 37°C), wear a latex finger cot and rub at a uniform speed for 60 seconds to simulate the shampooing process.

[0052] (5) Rinse the test area for 120 seconds with deionized water at 37°C and a flow rate of 2L / min.

[0053] (6) After rinsing, use standard filter paper to absorb excess moisture on the surface and equilibrate the sample in a constant temperature and humidity environment for 30 minutes.

[0054] (7) Scrape the measurement area (9cm) 2 The epidermis of the sample was placed in 10 mL of extraction solvent (70% methanol aqueous solution) and ultrasonically treated at 40 kHz for 30 minutes.

[0055] (8) The extract was centrifuged at 4000 rpm for 10 minutes, and the supernatant was filtered through a 0.22 µm filter membrane for analysis.

[0056] (9) High performance liquid chromatography (HPLC) was used to analyze the rosemary extract and rosmarinic acid was selected as the evaluation index.

[0057] (10) Prepare rosmarinic acid standard to establish a standard curve; inject the test solution into HPLC, measure the peak area and calculate the concentration.

[0058] (11) Based on the volume of the extract and the sample area, convert the results into the deposition amount per unit area (µg / cm²). 2 ).

[0059] The experimental data are shown in Table 3: Table 3: Results of Deposition of Active Ingredient (Rosmarinic Acid) in Test Example 1 ; (Note: ND = Not Detected) Conclusion: The quantitative data from Test Example 1 (Table 3) were used to verify the active material deposition mechanism of this scheme. The mechanism is based on the formation of a flocculent complex between the cationic polymer (polyquaternium-10) and the anionic surfactant during the dilution and rinsing process. The complex acts as a carrier to load the active extract in the E phase and deposits it on the substrate surface.

[0060] Data validated the essential components of the deposition mechanism: Example 5 (complete formulation, deposition rate 1.22 µg / cm³) 2 ) and Comparative Example 7 (lacking polyquaternium-10, deposition amount 0.20 µg / cm) 2 The comparison showed that in the system lacking cationic polymers, the active material deposition efficiency decreased by about 83.6%; this indicates that polyquaternium-10 is a key component of the deposition system, and without this component, a large amount of active ingredients are lost during the rinsing stage.

[0061] The data also demonstrate the dose-dependent nature of the system: rosmarinic acid deposition amounts in Examples 13 (total E phase 1.9%), 5 (total phase 2.8%), and 10 (total phase 3.3%) were 1.00, 1.22, and 1.45 µg / cm³, respectively. 2 The deposition efficiency increases with the total amount of active ingredients in the formulation, confirming that the deposition efficiency is positively correlated with the initial concentration of the E phase.

[0062] The deposition amount of Comparative Example 11 (containing only rosemary extract, 2.8%) was 2.62 µg / cm³. 2 The concentration of rosemary extract was higher than that in Example 5 (1.0% concentration, 1.22 µg / cm³ deposition). 2 This result corresponds to the concentration input of rosmarinic acid in the formulation, indicating that this deposition mechanism is equally effective for a single active ingredient.

[0063] The detection result of Comparative Example 8 (without E-phase active material) was ND (not detected), confirming the specificity of the detection method and ruling out substrate or reagent interference.

[0064] In summary, the data from Test Example 1 confirms that the flocculation complex constructed by this technical solution is an effective active ingredient deposition system. Its deposition efficiency depends on the presence of cationic polymers and is positively correlated with the concentration input of active ingredients in the formulation.

[0065] Test 2: System mildness test The experimental procedure is as follows: This test evaluates the in vitro irritant properties of the formulation by measuring its ability to dissolve zein.

[0066] Sample preparation: Group A (Example 5): The sample from Example 5 was accurately weighed and diluted with deionized water to prepare a 1.0% (w / v) sample dispersion.

[0067] Group B (Sodium C14-16 Olefin Sulfonate): Weigh out the sodium C14-16 olefin sulfonate raw material and prepare a solution with deionized water; make its effective concentration consistent with the theoretical value of sodium C14-16 olefin sulfonate concentration in Group A (Note: In Example 5, the amount of sodium C14-16 olefin sulfonate added was 23%, so in a 1.0% dispersion of Group A, its theoretical concentration was 0.23%).

[0068] Group C (negative control): Deionized water.

[0069] Group D (positive control): Sodium dodecyl sulfate (SDS) was weighed and prepared into a 0.5% (w / v) standard solution with deionized water.

[0070] Dissolution and reaction: (1) Accurately weigh 1.00g of zein powder and place it in four 100mL Erlenmeyer flasks.

[0071] (2) Accurately transfer 50 mL of the corresponding test solution (groups A, B, C, and D) into each conical flask.

[0072] (3) Place the conical flask in a 37°C constant temperature water bath shaker and shake at 100 rpm for 1 hour.

[0073] (4) After the reaction is complete, immediately transfer the sample to a centrifuge tube.

[0074] Separation and determination: (1) Centrifuge the sample after reaction at 4000 rpm for 15 minutes to precipitate the undissolved Zein protein.

[0075] (2) Carefully aspirate the supernatant.

[0076] (3) The absorbance (OD value) of the supernatant was measured at a wavelength of 280 nm using a UV spectrophotometer. The absorbance value is directly proportional to the concentration of dissolved Zein protein in the solution (i.e., the content of tyrosine and tryptophan), reflecting the irritant properties of the sample.

[0077] The experimental data are shown in Table 4: Table 4: Solubility test results of zein (OD@280nm) in Test Example 2 ; Conclusion: The data from Test Example 2 (Table 4) quantifies the denaturing ability of the formulation system of the present invention to proteins, thereby assessing its potential mildness.

[0078] Zein test results showed that the positive control group D (0.5% SDS) had the highest absorbance value (average 1.561), which is consistent with its standard characteristics as a strong irritant surfactant; the absorbance value of group B (sodium C14-16 olefin sulfonate, 0.23%) (average 0.982) was also at a high level, indicating that this anionic surfactant has significant protein denaturing ability and potential irritation when used alone.

[0079] The key data is in Group A (Example 5); the average absorbance of Group A was only 0.142, which was much lower than that of Group B (reduced by about 85.5%) and significantly lower than that of Group D; the value of Group A was closer to that of Group C (negative control, 0.012), indicating that the formulation system of Example 5 had extremely low solubility for Zein protein.

[0080] One of the innovations of this technical solution lies in the scientific compounding of surfactants; the solution uses sodium C14-16 olefin sulfonate as the main cleaning agent, as shown in Group B, which has high irritation when used alone; in Example 5, it is compounded with amphoteric, nonionic and amino acid surfactants such as cocamidomethyl MEA, cocamidopropyl hydroxysulfobetaine, and sodium lauroyl glutamate in a specific ratio.

[0081] The data in Table 4 confirms that this compounding strategy is not simply a combination of the properties of each component; the solubility of Zein in group A (complete formulation) is much lower than that in group B (single key anion); this indicates that the compounding system effectively reduces the affinity and denaturing ability of sodium C14-16 olefin sulfonate for proteins through the interaction between different types of surfactants (e.g., forming mixed micelles, reducing the concentration of monomeric surfactants).

[0082] Therefore, Test Example 2 confirms that, while ensuring cleaning power, this solution controls the overall in vitro irritation of the formulation to an extremely low level through the synergistic compounding of the surfactant system, thus verifying the mildness of the solution.

[0083] Test 3: Hair Bundle Moisturizing Effect Test Test basis: ALSSOP-042 "Operating Procedure for Weighing Hair Moisture Content" Experimental objective and principle: The product's hair ties moisturizing effect was verified by weighing the hair ties using the test samples, positive control, and negative control group (before use, 1h, 2h, and 4h after use) and comparing the hair ties moisturizing rate after use.

[0084] Hair strands have the ability to absorb moisture, and their moisture content can be determined by weighing them. Changes in the moisture content of the hair strands reflect the effect of the test sample on their moisturizing ability. In the hair strand moisturizing test, glycerin solution was used as a positive control. After using the test sample, the moisture retention rate of the hair strands was higher than that of the positive control group, indicating that the test sample has a moisturizing effect on the hair strands.

[0085] Experimental materials and instruments: Black, flat hair strands in their original state, 15cm, 2.5g; electronic balance (accuracy 0.001g).

[0086] Test environment: Temperature: 21.0℃±1.0℃; Humidity: 50%±10%RH.

[0087] Test plan: 1. Randomly select flat hair bundles (2.5g, 15cm, healthy hair bundles) matching the number of test samples and equilibrate them for 24 hours in a constant temperature and humidity environment of (21±1)℃ and (50±10)%RH.

[0088] 2. The hair bundles were randomly divided into a negative control group (deionized water), a positive control group (3% glycerol solution), an example group (Examples 1-8), and a comparative group (Comparative Examples 1-6).

[0089] 3. Weigh all hair bundles initially and record the mass m0.

[0090] 4. After washing the hair, wipe off any excess water.

[0091] Example group and comparative group: Take 0.1g / g of the corresponding sample from each hair strand and apply it to the hair strand. Leave the sample on for 2 minutes and rinse with running water for 30 seconds. Repeat the operation 3 times.

[0092] Positive control group: The hair strands were immersed in the positive control for 2 minutes and rinsed with running water for 30 seconds; the procedure was repeated 3 times.

[0093] Negative control group: each hair strand was rinsed with running water for 30 seconds; the procedure was repeated 3 times.

[0094] 5. The sample group and the control group were placed in a constant temperature and humidity environment.

[0095] 6. Weigh the hair bundles at 1h, 2h, and 4h respectively, and record the masses m1, m2, and m4.

[0096] The hair bundle moisture retention rate at different time points is calculated using the formula: Moisturizing rate (%) = [(mt-m0) / m0] × 100; where mt is the mass of the hair bundle after using the sample for time t; and m0 is the mass of the hair bundle before using the sample.

[0097] The experimental data are shown in Table 5: Table 5: Results of Hair Strand Moisture Retention Rate Test ; Conclusion: Refer to Appendix Figure 1 The gravimetric moisturizing test data in Table 5 objectively evaluated the water-holding capacity of different formulation compositions for hair strands.

[0098] First, the moisturizing rate (T4h value between 11.28% and 16.85%) of all examples (1-8) and comparative examples (1-6) after 4 hours was significantly higher than that of the positive control (glycerol solution, 7.42%); this indicates that the basic formulation platform used in this invention has good moisturizing properties.

[0099] Secondly, the data clearly point to the superiority of Example 5; Example 5 showed the highest moisturizing rate at all time points (T1h, T2h, T4h), with a T4h value of 16.85%, which was significantly higher than all other groups.

[0100] By comparing Example 5 with Comparative Examples 4, 5, and 6, the synergistic effect of the active ingredient combination can be confirmed. Example 5 (16.85%) contains three extracts: lychee, aloe vera, and rosemary. The T4h moisturizing rates of Comparative Examples 4 (lychee missing, 14.35%), 5 (aloe vera missing, 14.10%), and 6 (rosemary missing, 13.90%) all showed a significant decrease. This indicates that the simultaneous presence of the three extracts is necessary to achieve the best moisturizing effect, and the specific compound combination in the formulation has a synergistic effect.

[0101] Furthermore, the comparison between Example Groups (1-8) and Comparative Groups (1-3) further supports the effectiveness of this specific combination of plant extracts; Example 5 (16.85%) showed better moisturizing performance than formulations using conventional soothing agents dipotassium glycyrrhizate (Comparative Example 1, 15.27%) or allantoin (Comparative Example 2, 14.28%), and was far superior to formulations using ginger root extract (Comparative Example 3, 11.28%).

[0102] Furthermore, the synergistic effect of the compound can be verified again by comparing the examples with the complete components and the examples with missing components. For example, the moisturizing effect of the complete components in Example 5 (T4h: 16.85%) and Example 1 (T4h: 12.75%) is better than that of the examples with missing single components in Example 2 (11.71%), Example 3 (11.65%), Example 4 (11.57%), Example 6 (14.65%), Example 7 (14.39%) and Example 8 (14.18%). This set of internal comparison data once again confirms the effectiveness of the three-component compound, in which the specific ratio of Example 5 (1% lychee, 0.8% aloe vera, and 1% rosemary) achieved the best effect.

[0103] In summary, the data from Test Example 3 confirms that the specific combination of litchi peel extract, aloe vera extract, and rosemary extract used in the embodiments of the present invention, especially the ratio in Example 5, can provide hair styling moisturizing effects superior to those of single-component absence, conventional active ingredients, or positive control groups.

[0104] Test 4: External Soothing Efficacy Test Test basis: HMC-WI-029 "Hyalurase Inhibition Rate" Experimental objective and principle: Hyaluronidase is a specific enzyme that cleaves hyaluronic acid and is involved in allergic reactions, showing a strong correlation with histamine release from mast cells. The soothing effect of a test sample can be determined using the hyaluronidase inhibition rate; a higher inhibition rate indicates a stronger soothing effect, and vice versa.

[0105] This experiment references the laboratory method (HMC-WI-029 hyaluronidase inhibition rate). The results of the hyaluronidase inhibition rate test of the test sample and the negative control were compared. If the inhibition rate of the test sample was higher than that of the negative control and the difference was statistically significant (P<0.05), the test sample could be considered to have a soothing effect.

[0106] This test method is an in vitro method, applicable to evaluating cosmetics that claim to achieve soothing effects by inhibiting hyaluronidase.

[0107] Experimental indicators: Experimental indicator: Hyaluronidase inhibition rate.

[0108] Judgment criteria: If the hyaluronidase inhibition rate of the sample is higher than that of the negative control and the difference is statistically significant (P<0.05), then the test sample can be considered to have a soothing effect.

[0109] Experimental materials and methods: 1. Instruments and equipment: BSA224S analytical balance; L6s ultraviolet spectrophotometer.

[0110] 2. Reagents: Hyaluronidase, BR; Sodium hyaluronate, BR.

[0111] 3. Test methods: (1) Processing of control materials and test samples: Sample group: Dilute with pure water to a sample concentration of 2%, let stand, and then take the supernatant for testing.

[0112] Control (dipotassium glycyrrhizate, purity ≥98%): diluted with pure water to a positive control concentration of 3%.

[0113] Negative control: pure water.

[0114] (2) Experimental operation steps: Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group should have 3 replicates. Add different reagent solutions to each of the four groups, shake well, and let stand at room temperature for 30 minutes to develop color. Measure the absorbance value at a wavelength of 528 nm using a UV spectrophotometer.

[0115] (3) Calculation formula: In the formula, The absorbance of the reaction solution without the sample; The absorbance is the absorbance of the reaction solution that does not contain the sample or enzyme. The absorbance is the absorbance of the reaction solution containing the sample and the enzyme. The absorbance is for the reaction solution containing the sample and the reaction solution without the enzyme. (4) Data Analysis: Statistical analysis software was used to perform statistical analysis of the data. Two-tailed tests were used for all statistical methods, with a significance level of α=0.050. The Shapiro-Wilk Test was used to test the significance of the normality of the data. If Sig. (two-tailed) > 0.050, the data were normally distributed, and a T-test was performed; if Sig. (two-tailed) ≤ 0.050, the data were not normally distributed, and a Wilcoxon test was performed. The comparison between the test samples, positive controls, and negative controls was performed using independent samples T-tests or rank-sum tests. All the above statistical analyses were two-tailed tests with a significance level of α=0.05; P>0.05 indicated no significant difference between the two groups; P<0.05 indicated a significant difference between the two groups.

[0116] The experimental data are shown in Table 6: Table 6: Results of in vitro soothing efficacy (hyaluronidase inhibition rate) test ; Conclusion: The data in Table 6 provide a quantitative assessment of the in vitro soothing efficacy of the formulation; the p-values ​​for all examples (1-8) and comparative examples (1-6) were <0.05, indicating that they all had statistically significant hyaluronidase inhibitory activity compared with the negative control (-2.436%); the positive control (66.071%) showed the expected strong inhibitory effect.

[0117] Example 5 exhibited the highest inhibition rate (15.624%) among all examples and comparative examples, demonstrating the superiority of its formulation.

[0118] Comparing Example 5 with Comparative Examples 4, 5, and 6 confirms the synergistic effect of the E-phase active ingredient combination. The inhibition rates of Comparative Example 4 (lychee deficiency, 14.695%), Comparative Example 5 (aloe deficiency, 14.268%), and Comparative Example 6 (rosemary deficiency, 14.069%) were all lower than those of Example 5. This data indicates that the simultaneous presence of the three components—lychee peel extract, aloe extract, and rosemary extract—is necessary to achieve optimal in vitro soothing activity, confirming the synergistic effect of this specific compound combination.

[0119] When Example 5 was compared with Comparative Examples 1, 2, and 3, the inhibition rate of Example 5 (15.624%) was higher than that of the formulations containing conventional soothing agents such as dipotassium glycyrrhizate (Comparative Example 1, 14.069%), allantoin (Comparative Example 2, 11.069%), or ginger root extract (Comparative Example 3, 11.068%).

[0120] Furthermore, by comparing the examples with the complete components and the examples with missing components, the synergistic effect of the compound can be verified again. For example, the soothing activity of the complete components in Example 5 (inhibition rate: 15.624%) and Example 1 (11.265%) is generally better than that of the examples with missing single components in Example 2 (10.965%), Example 3 (10.268%), Example 4 (10.068%), Example 6 (13.695%), Example 7 (13.059%), and Example 8 (12.956%). This set of internal comparison data once again confirms the effectiveness of the three-component compound, in which the specific ratio of Example 5 achieves the optimal effect.

[0121] Data from Test Example 4 confirms that the specific combination of litchi peel extract, aloe vera extract, and rosemary extract used in the embodiments of the present invention, especially the ratio in Example 5, can provide superior in vitro soothing effects compared to single-component deficiencies or the addition of other conventional active ingredients.

[0122] Test 5: Foam Performance Test Test basis: Refer to the foam volume determination method (Ross-Miles method) in QB / T1974 "Shampoo".

[0123] Experimental Objective and Principle: To evaluate the foaming ability and foam persistence of shampoo products by determining the foam volume and foam stability of the sample dilution using the Ross-Miles method. Foam performance is a key indicator of the user experience of shampoo products.

[0124] Experimental materials and instruments: Ross-Miles foaming apparatus (with constant temperature jacket); constant temperature water bath; stopwatch; 1000mL graduated cylinder.

[0125] Test environment: Temperature: (25.0±2.0)℃.

[0126] Test plan: (1) Sample preparation: All test samples (examples and comparative examples) were prepared into a 1.0% (w / v) test solution using standard hard water (150ppm CaCO3) and placed in a 25°C water bath for constant temperature.

[0127] (2) Instrument preparation: Ensure that the Ross-Miles foaming instrument is clean and dry, and circulate 25°C constant temperature water to maintain the jacket temperature.

[0128] (3) V0 determination (initial foam volume): Accurately transfer 50.0 mL of test solution into the instrument’s reservoir tube (receiving tube); accurately transfer 200.0 mL of the same test solution into the dropper tube above.

[0129] (4) Open the stopcock of the dropper so that the solution flows down within a specified time and impacts the solution in the storage tube; after the liquid surface stops undulating, immediately read the total volume of the foam column (including 50 mL of liquid) and record it as V0.

[0130] (5) V5 measurement (foam volume in 5 minutes): Keep it in a static state, time for 5 minutes after reading V0, and read the total volume of the foam column again, which is recorded as V5.

[0131] The experimental data are shown in Table 7: Table 7: Test Results of Foam Performance (Ross-Miles Method) for Test Example 5 ; Conclusion: The data from Test Example 5 (Table 7) evaluated the foaming performance of the formulation, which is a fundamental indicator of the user experience of shampoo products.

[0132] Data shows that all tested samples exhibited high initial foam volume (V0 value greater than 400 mL), indicating that the selected surfactant system (mainly sodium C14-16 olefin sulfonate) has high foaming ability.

[0133] Compared with Comparative Example 5 (V0=412mL) and Comparative Example 7 (V0=441mL, without polyquaternium-10), the initial foam volume of Example 5 was slightly lower; this phenomenon is as expected because the cationic polymer (polyquaternium-10) in this scheme interacts with the anionic surfactant during dilution to form a flocculent complex; this process consumes some of the free surfactant, which may slightly affect the initial foaming rate.

[0134] The V0 value of Comparative Example 8 (V0=426mL, without E-phase active ingredient) was higher than that of Example 5, indicating that the addition of E-phase active ingredient (plant extract) also had a slight inhibitory effect on foam.

[0135] By comparing Example 5 (V0=412mL), Comparative Example 9 (V0=415mL, lychee only), and Comparative Example 10 (V0=410mL, aloe only), the foam volume data of the three were very close; this indicates that the effect on foam performance is similar whether a compound extract (Example 5) or a single extract (Comparative Examples 9 and 10) is used; this confirms that the slight decrease in foam performance is mainly due to the introduction of the active ingredient (extract) itself, rather than a specific compound combination.

[0136] Despite slight effects on V0, all formulations (including Examples 5 and 10) maintained high foam volumes (V5) after 5 minutes, and foam stability (V5 / V0 ratio) remained in the excellent range (approximately 90% to 93%).

[0137] The conclusion of Test Example 5 is that this technical solution, while introducing a flocculation deposition system to achieve efficient deposition of active ingredients (Test Example 1) and efficacy (Test Examples 3 and 4), does not sacrifice the product's foaming performance. The formulation maintains a high level of foam volume and excellent foam stability, ensuring a good user experience.

Claims

1. A moisturizing and soothing composition, characterized in that, The composition comprises: Sodium C14-16 olefin sulfonate, with a content of 20% to 25%; Cationic polymer, with a content of 0.3% to 0.7%; Lychee peel extract; Aloe vera extract; Rosemary extract; The total weight percentage of the litchi peel extract, the aloe vera extract, and the rosemary extract is 1.9% to 3.3%.

2. The moisturizing and soothing composition according to claim 1, characterized in that, The composition comprises: Lychee peel extract, content 1%; Aloe vera extract, content 0.8%; Rosemary extract, content 1%.

3. The moisturizing and soothing composition according to claim 1, characterized in that, The cationic polymer comprises polyquaternary ammonium salt-10.

4. The moisturizing and soothing composition according to claim 3, characterized in that, The content of the polyquaternium-10 is 0.3% to 0.5%.

5. The moisturizing and soothing composition according to claim 1, characterized in that, The composition further comprises at least one auxiliary surfactant selected from cocamidomethyl MEA, cocamidopropyl hydroxysulfonate betaine, cocamidopropyl betaine, sodium lauroyl glutamate, and sodium lauroyl sarcosinate.

6. The moisturizing and soothing composition according to claim 1, characterized in that, The composition further comprises pyrrolidone ethanolamine salt in a content of 0.25%.

7. The moisturizing and soothing composition according to claim 1, characterized in that, The composition comprises, by weight percentage, the following components: Sodium C14-16 olefin sulfonate: 23%; Cocamidopropyl hydroxysulfonate betaine: 11%; Sodium lauroyl sarcosinate: 8%; Cocamidopropyl betaine: 4.4%; Sodium lauroyl glutamate: 4%; Cocamide methyl MEA: 2.5%; Polyquaternium-10: 0.3%–0.5%; Guar gum hydroxypropyltrimethylammonium chloride: 0%–0.2%; Piroctone ethanolamine salt: 0.25%; PPG-3 octyl ether: 0.5%; Polyquaternium-7: 0.5%; Lychee peel extract: 0.3%–1.2%; Aloe vera extract: 0.4%–0.9%; Rosemary extract: 0.5%–1.2%; Glycerin: 1%; Citric acid: 0.4%; Disodium EDTA: 0.1%; Phenoxyethanol: 0.5%; Sodium benzoate: 0.4%; Fragrance: 0.8%; Menthol: 0.1%; The remaining water.

8. A method for preparing the moisturizing and soothing composition according to any one of claims 1 to 7, characterized in that, Includes the following steps: The A-phase raw materials are mixed, which include water, sodium C14-16 olefin sulfonate, cationic polymer, and at least one auxiliary surfactant. The mixture is heated to 80°C to 85°C and homogenized at a speed of 2000 rpm to 2800 rpm to obtain the A-phase mixture. Cool the A-phase mixture to 42℃~45℃; Add phase E, which contains lychee peel extract, aloe vera extract and rosemary extract, to the cooled phase A mixture, and stir until homogeneous to obtain the moisturizing and soothing composition.

9. The method according to claim 8, characterized in that, The heating temperature of the A-phase mixture is 80°C, the homogenization speed is 2000 rpm, and the cooling temperature of the A-phase mixture is 45°C.

10. Use of the composition according to any one of claims 1 to 7 in the preparation of a shampoo product for moisturizing or soothing the scalp.

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