16 results about "Skin biopsy" patented technology

The application discloses a skin biopsy sampling device and belongs to the technical field of medical devices, which comprises two adjustable-height mounting racks, spring telescopic rods are mounted on the two mounting racks, L-shaped fixing rods are fixed at one end of the spring telescopic rods, a positioning ring is fixed at the bottom ends of the two fixing rods, a sampling mechanism is arranged between the two fixing rods, a piston sampling cylinder is movably arranged on the sampling mechanism, a ring cutter is fixed at the bottom end of the piston sampling cylinder, and a scale is arranged on the side wall of the piston sampling cylinder; the application is simple to operate and high in precision, has a pre-cooling function, directly reduces the sensitivity of skin nerve endings by using low temperature, reduces the discomfort of a patient, can realize automatic replacement of gauze at the same time, uses brand-new gauze to contact the skin every time, completely avoids the risk of bacterial residue and cross infection, and is suitable for multiple sampling.

The invention provides a skin cutting device, and belongs to the technical field of medical equipment.The skin cutting device comprises a shell, a linear motion mechanism and a tool bit, and the shell is provided with a containing space with a first opening; the linear motion mechanism is installed in the containing space, and the motion end of the linear motion mechanism can do reciprocating linear motion in the direction pointing to the first opening. The tool bit is detachably connected to the moving end from the first opening, and the tool bit can be driven by the moving end to do reciprocating rectilinear motion so as to cut the skin. According to the skin cutting device provided by the invention, compared with bare-handed skin cutting, a medical worker can conveniently and quickly cut the skin of a patient through the skin cutting device, so that the operation efficiency is improved, and the pain of the patient is effectively reduced; by reasonably designing the specification and size of the cutter head, a skin sample with standard size and thickness can be cut, and the inspection precision of skin biopsy is improved.

The invention relates to the field of diagnostic kits, in particular to application of an IGHV family molecule as a diagnostic marker in preparation of a diagnostic kit for psoriasis (PSO) and arthropathy type psoriasis (PsA), and the IGHV family molecule is selected from IGHV3-72, IGHV5-51, IGHG3, IGHV1-69, IGHV3-73 and / or IGHV3-9. Wherein the prediction efficiency of the IGHG3 is the highest, and the area under the curve (AUC) is 0.826 (95% CI: 0.693-0.959). IGHV family molecules are specifically expressed in psoriasis, the diagnosis specificity is high, and the cross reaction with other autoimmune diseases can be reduced; the invasive operation of skin biopsy can be avoided through nail sample detection; the kit is suitable for early screening of PSO, early diagnosis and prediction of PsA and dynamic monitoring of curative effect, and is widely applied.

Vitiligo is a complex auto-immune skin disease resulting in melanocytes loss and skin depigmentation. Following-up on their previous work identifying mitochondrial DNA (mtDNA) in skin biopsies from some vitiligo patients, the inventors demonstrate here that melanocytes from non-lesional skin present elevated numbers of mtDNA variants associated with mtDNA release and can be classified as function of their number of mtDNA variants in low-variant (LV) and high-variant (HV) load. Vitiligo HV melanocytes display increased ROS production, reduced antioxidant capacity, elevated mitochondrial function and mtDNA release as compared to Vitiligo LV and healthy melanocytes. Sensing of mtDNA by the cGAS-STING pathway results in pro-inflammatory cytokine and chemokines production promoting the recruitment of cytotoxic CD8+ T cells. This cascade of events can be blocked with TBK1 inhibitors suggesting new therapeutic approaches for sub-population of vitiligo patients displaying HV mtDNA phenotype. The present invention relates to method for the treatment of vitiligo in a subject in need thereof comprising a step of administrating to the subject a therapeutically effective amount of TANK-binding kinase 1 (TBK1) inhibitor.

The invention provides a human skin sweat gland three-dimensional shape obtaining method and system and electronic equipment, and relates to the technical field of optical coherence tomography and medical image segmentation, and the method comprises the steps: obtaining an initial to-be-detected region skin OCT image through a spectral domain optical coherence tomography system; preprocessing the skin OCT image of the initial to-be-detected area, wherein preprocessing comprises but is not limited to one or more of cutting, overturning, size adjustment and contrast adjustment; inputting the preprocessed skin OCT image of the initial to-be-detected area into a three-dimensional segmented skin sweat gland network model trained in advance based on deep learning, and performing three-dimensional segmentation on the preprocessed skin OCT image of the initial to-be-detected area by the deep learning model to obtain a three-dimensional segmentation result of sweat glands; according to the three-dimensional shape obtaining method, the three-dimensional shape information of the sweat gland of the human skin can be obtained in a real-time and non-invasive mode, the two-dimensional, in-vitro and destructive limitation of a traditional skin biopsy technology is overcome, and the accuracy and efficiency of sweat gland shape analysis are remarkably improved.

The utility model discloses a skin biopsy sampling tool, which relates to the technical field of medical treatment, and comprises a tool main body, two cutting plates fixedly connected to the lower end of the main body and symmetrically arranged, and sampling tweezers, the lower end of the main body is provided with a mounting groove, the upper end of the sampling tweezers is mounted in the mounting groove, and the lower end of the sampling tweezers extends out of the lower end of the main body. The extending end of the sampling tweezers and the two cutting plates are combined into an annular structure, a limiting column used for limiting the sampling tweezers is fixedly installed in the installation groove, the annular structure formed by the sampling tweezers and the cutting plates is rotatably inserted into the skin sampling position, the two sides of the sampling tweezers are pressed, the sampling tweezers clamp a skin sample, and then sampling tissue can be pulled out from the skin surface. According to the skin biopsy sampling tool, the combination of the punching sampler and the tweezers is completed, and the problems that when an existing skin biopsy sampling tool is used for sampling, the punching sampler and the tweezers need to be alternately used, the operation time is prolonged, and meanwhile the operation complexity is increased are solved.

Various methods for producing skin grafts for rehabilitation of skin defects are, carried out in a closed system operated and controlled in an automated manner. A skin biopsy may be separated into a dermis layer and an epidermis layer prior to formation of a skin graft. A total skin graft area produced may be 9 times to 1000 times greater than a total skin biopsy area that provides cells for the graft. Differences in density between dermis and epidermis may aid in separation between a dermis and an epidermis layer.

Various methods for producing skin grafts for rehabilitation of skin defects are carried out in a closed system operated and controlled in an automated manner. A skin biopsy may be separated into a dermis layer and an epidermis layer prior to formation of a skin graft. A total skin graft area produced may be 9 times to 1000 times greater than a total skin biopsy area that provides cells for the graft. Differences in density between dermis and epidermis may aid in separation between a dermis layer and an epidermis layer.

The invention discloses a skin biopsy sampling device, and belongs to the technical field of medical apparatuses and instruments, the skin biopsy sampling device comprises two height-adjustable mounting racks, spring telescopic rods are mounted on the two mounting racks, L-shaped fixing rods are fixed to one ends of the spring telescopic rods, a positioning ring is fixed to the bottom ends of the two fixing rods, and the positioning ring is fixed to the bottom ends of the spring telescopic rods. A sampling mechanism is placed between the two fixing rods, a piston sampling barrel is movably arranged on the sampling mechanism, an annular cutter is fixed at the bottom end of the piston sampling barrel, and scales are arranged on the side wall of the piston sampling barrel; during sampling, the operation is simple, the precision is high, the precooling function is achieved, the sensitivity of skin nerve endings is directly reduced through low temperature, the discomfort of a patient is relieved, automatic gauze replacement can be synchronously achieved, brand new gauze is used for making contact with the skin during sampling each time, the risks of bacterial residues and cross infection are thoroughly avoided, and the device is suitable for multiple times of sampling.

In the present study, the Inventors propose that characterizing fibroblasts derived from individuals of very different ages, regarding their Structure, Inflammation, and Metabolism (SIM) signatures, and assessing their stress response to challenges mimicking life stresses, would enhance their understanding of the aging process and frailty syndrome and intrinsic capacity. To address these inquiries, they used fibroblasts obtained from skin biopsies of 133 male and female volunteers from the INSPIRE human translational cohort (INSPIRE-T cohort) 21, spanning ages 20 to 96 years, and encompassing various frailty statuses (robust, pre-frail, frail). Through this thorough characterization, they pinpointed markers specifically associated with aging, intrinsic capacity or indicators of frailty syndrome. The present invention relates to an in vitro method for predicting intrinsic capacity decline and / or frailty in a subject comprising the step of determining the level of extracellular periostin in a sample comprising fibroblasts obtained from said subject.

The invention provides a tool bit and a skin cutting device, and belongs to the technical field of medical equipment.The tool bit comprises a tool bit body, a cover plate and a tool handle, the tool bit body is in a thin-wall barrel shape, one end of the tool bit is arranged to be a tool edge, and the tool bit body linearly shrinks from the end provided with the tool edge to the other end; the cover plate covers the end, away from the knife edge, of the knife head body, and an exhaust port is formed in the cover plate. The cutter handle is arranged on the side, away from the cutter head body, of the cover plate. The knife head and the skin cutting device provided by the invention are beneficial to formation of a skin edge slight eversion upheaval state in a skin suturing process, are convenient to carry out embedded mattress type subcutaneous suturing, form a state that an incision forms a section slight outward inclination state from a body surface to a dermis direction, are beneficial to beauty healing of the skin incision, improve the operation precision of skin biopsy, and improve the operation efficiency of the skin biopsy. And the benign healing rate and the beauty healing rate of the incision are improved.

The application discloses a kind of for neuron intranuclear inclusion disease vascular sympathetic nerve injury evaluation method;Belong to the field of biological science and technology, its operating steps are as follows: skin biopsy, immunofluorescence staining, slide scanning, calculate vascular adrenergic small fiber density.The application obtains tissue by skin biopsy, is photographed after being given immunofluorescence staining, and the density of the sympathetic adrenergic small nerve of the superficial layer of dermis blood vessel marked by TH antibody is calculated using Image J software, to accurately assess whether the receptor of neuron intranuclear inclusion disease exists sympathetic nerve injury and its severity.

The invention discloses a portable skin biopsy gun. The portable skin biopsy gun comprises a mounting base, a launching base, two hammers, two triggering units and two elastic pieces, wherein the hammers, the triggering units and the elastic pieces are in one-to-one correspondence. The launching base is mounted on the mounting seat; an inner launching groove is formed in the launching base, and the inner launching groove comprises a needle tube section, a sliding section and a power section in the first linear direction; the biopsy needle is installed on the needle tube section and extends into the sliding section. The two hammers are slidably connected to the sliding section in the first linear direction, and the front ends of the hammers are aligned with an outer sheath needle and an inner core needle in the biopsy needle respectively. The trigger unit is movably connected to the mounting seat, and the working end of the trigger unit extends into the sliding section and extends into a first positioning hole / second positioning hole formed in the side wall of the corresponding outer sheath needle / inner core needle. The elastic piece is installed on the power section and connected with the driving hammer and the launching base, and when the trigger unit enables the working end of the trigger unit to retreat from the first positioning hole / the second positioning hole under the action of external force, the elastic piece drives the driving hammer to slide forwards and pushes the outer sheath needle / the inner core needle to move forwards.

The invention relates to the field of diagnostic kits, in particular to application of a complement component as a diagnostic marker in preparation of a psoriasis diagnostic kit, and the complement component is selected from C1R, C2, C1QC, C7, C6, C9, C4A and / or C1QB. The complement component is specifically expressed in psoriasis, the diagnosis specificity is high, and the cross reaction with other autoimmune diseases can be reduced; the invasive operation of skin biopsy can be avoided through nail sample detection; the kit is suitable for PSO early screening, PSA differential diagnosis and dynamic curative effect monitoring, and is wide in application.

The invention relates to a kit for detecting transthyretin amyloidosis in vitro and a preparation and purification method of a substrate protein, the kit comprises the substrate protein, a sample extraction reagent of total protein in skin tissue, a reaction reagent for real-time oscillation induced conversion (RT-QuIC), a negative reference substance and a positive reference substance, the substrate protein comprises at least one of a WT-TTR recombinant protein and a D38A-TTR recombinant protein, the WT-TTR recombinant protein and the D38A-TTR recombinant protein correspond to a sequence SEQ ID NO 1 and a sequence SEQ ID NO 2 respectively, and accurate diagnosis of transthyretin amyloidosis is achieved through skin biopsy in combination with an RT-QuIC technology.

Vitiligo is a complex auto-immune skin disease resulting in melanocytes loss and skin depigmentation. Following-up on their previous work identifying mitochondrial DNA (mtDNA) in skin biopsies from some vitiligo patients, the inventors demonstrate here that melanocytes from non-lesional skin present elevated numbers of mtDNA variants associated with mtDNA release and can be classified as function of their number of mtDNA variants in low-variant (LV) and high-variant (HV) load. Vitiligo HV melanocytes display increased ROS production, reduced antioxidant capacity, elevated mitochondrial function and mtDNA release as compared to Vitiligo LV and healthy melanocytes. Sensing of mtDNA by the cGAS-STING pathway results in pro-inflammatory cytokine and chemokines production promoting the recruitment of cytotoxic CD8+ T cells. This cascade of events can be blocked with NRF2 activators suggesting new therapeutic approaches for sub-population of vitiligo patients displaying HV mtDNA phenotype. The present invention relates to method for the treatment of vitiligo in a subject in need thereof comprising a step of administrating to the subject a therapeutically effective amount of Nuclear factor erythroid 2 -related factor 2 (NRF2) activator.