Method for predicating gene age and disease susceptibility and kit

A susceptibility, kit technology for medical biotechnology and genetic diagnosis

Inactive Publication Date: 2015-06-10
冯文峰 +3
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no related reports on methods and reagents for predicting genetic age and disease susceptibility by using genetic polymorphisms at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for predicating gene age and disease susceptibility and kit
  • Method for predicating gene age and disease susceptibility and kit
  • Method for predicating gene age and disease susceptibility and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Method for Predicting Genetic Age and Disease Susceptibility

[0028] The sample used in this embodiment is: the subject's genomic DNA, and the sample number is DNA011.

[0029] Among them: collection of blood samples and extraction of genomic DNA

[0030] Blood samples were collected from healthy individuals with their consent. Take 0.5ml of anticoagulated whole blood and put it into a 1.5ml centrifuge tube, add double volume of red blood cell lysate (0.15mol / L NH4CL, 0.13mol / L EDTA), and mix well. Let stand at room temperature for 20 minutes, add DNA extraction solution (10mmol / L TrisCl, 0.1mmol / L EDTA, 1% SDS), boil in boiling water, set aside.

[0031] The method for predicting genetic age and disease susceptibility includes the following steps:

[0032] 1. Select the genetic locus to predict the genetic age

[0033] 1) Enter the PubMed database http: / / www.ncbi.nlm.nih.gov / pubmed / ;

[0034] 2) Click "Advanced" advanced search;

[0035] 3) Select...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for predicating gene age and disease susceptibility and a kit, and belongs to the technical field of medical biology and gene diagnosis. The method comprises the following steps: extracting cellular genome DNA of a host; testing polymorphic sites of genes (RTEL1 gene, ACYP2 gene, TERC gene, TERT gene, NAF1 gene, OBFC1 gene and ZNF208 gene) of a subject; and predicating the gene age and the disease susceptibility of the subject. According to the method, the correlation between the polymorphic sites of seven genes and senescence gene is described for the first time; the method can be applied to predication, auxiliary diagnosis and treatment of diseases caused by the senescence gene, and can be also applied to research and development of new drugs in order to guide the subject to start a healthy life.

Description

technical field [0001] The invention belongs to the technical fields of medical biotechnology and gene diagnosis, and more specifically, the invention relates to a method and a kit for predicting genetic age and disease susceptibility. Background technique [0002] Aging in humans is a complex phenotype with multiple determinants. Although non-genetic factors (including environmental pollution, disease, irregular living habits, etc.) are very important, up to 40% of the changes in human aging can be explained by genetic differences. [0003] A better understanding of the mechanisms of aging has important implications for reducing our risk of age-related diseases and disabilities. There are many biologically plausible candidate genes for human longevity, but so far only one discovery, that of the ApoE gene, can be broadly replicated across multiple populations. This gene has a pervasive effect on aging phenotypes, particularly cardiovascular disease and dementia, thereby af...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 冯文峰佘志勇陈建兴方海庆
Owner 冯文峰
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap