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Creatinine detection kit

A kit and creatinine technology, applied in the field of medical testing and determination, can solve problems such as poor mixing stability, and achieve the effects of good stability, wide application range and guaranteed accuracy

Active Publication Date: 2011-11-23
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of this, the object of the present invention is to solve the problem of poor stability of mixing 2,4,6-trinitrophenol and alkaline buffer solution in the existing kits for detecting creatinine, and to provide a reagent for detecting creatinine with good stability box

Method used

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Examples

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Effect test

Embodiment 1

[0023] Example 1: The kit for detecting creatinine of the present invention.

[0024] The kit for detecting creatinine of the present invention is a single liquid reagent, including: 0.1% Triton X-100, volume fraction of 10% dimethyl sulfoxide, 6mmol / L 2,4,6-trinitrophenol, 100mmol / L sodium silicate, 50mmol / L Tris, 28mmol / L sodium carbonate, 2.8mmol / L sodium bicarbonate and 100mmol / L sodium hydroxide.

[0025] Standard solution: 200μmol / L creatinine.

Embodiment 2

[0026] Example 2: The kit for detecting creatinine of the present invention.

[0027] The kit for detecting creatinine of the present invention is a single liquid reagent, including: 0.15% Triton X-100, volume fraction of 10.5% dimethyl sulfoxide, 10mmol / L 2,4,6-trinitrophenol, 100mmol / L Tris, 30mmol / L sodium carbonate, 3.0mmol / L sodium bicarbonate and 120mmol / L sodium hydroxide.

[0028] Standard solution: 150μmol / L creatinine.

Embodiment 3

[0029] Example 3: The kit for detecting creatinine of the present invention.

[0030] The kit for detecting creatinine of the present invention is a single liquid reagent, including: 0.2% Triton X-100, 9.5% dimethyl sulfoxide, 3mmol / L 2,4,6-trinitrophenol, 120mmol / L sodium silicate, 50mmol / L Tris, 25mmol / L sodium carbonate, 2.5mmol / L sodium bicarbonate and 80mmol / L sodium hydroxide.

[0031] Standard solution: 250μmol / L creatinine.

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Abstract

The invention relates to the technical field of medical detection and discloses a creatinine detection kit. The creatinine detection kit provided by the invention comprises 2,4,6-trinitrophenol, a buffer solution with the pH ranging from 12.5 to 13.5 and a mixture of triton X-100 and dimethyl sulfoxide. The mixture of triton X-100 and dimethyl sulfoxide can surround the molecules of 2,4,6-trinitrophenol so as to slow down the fast contact of 2,4,6-trinitrophenol with NaOH in a buffer system with the pH ranging from 12.5 to 13.5, so that the stability of reagents is increased and the accuracy of a creatinine detection result is guaranteed. Tests show that the kit disclosed by the invention has the advantages of good stability, long retention period, high creatinine detection accuracy, goodcreatinine detection precision and wide linear range. The kit disclosed by the invention has a wide applicable scope, is convenient for popularization and use, and can be applied to the determinationof creatinine content in serum in hospitals at all levels, departments of health and epidemic prevention and research and development institutes of medical biology.

Description

Technical field [0001] The invention relates to the technical field of medical testing and determination, and in particular to a kit for detecting creatinine. Background technique [0002] Creatinine (Cr) is a product of muscle metabolism in the human body, and 1mg creatinine can be produced for every 20g of muscle metabolism. Blood creatinine comes from both exogenous and endogenous. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of metabolism of muscle tissue in the body. When the meat intake is stable, the body's muscle metabolism does not change significantly, and the production of creatinine will be relatively constant. [0003] In muscles, creatine slowly forms creatinine through an irreversible non-enzymatic dehydration reaction, which is then released into the blood and excreted in urine. Therefore, blood creatinine is closely related to the total amount of muscle in the body and is not easily affected by diet. Cr...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD
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