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Extracting technology of dictyophora indusiata volva colloid polysaccharide

An extraction process, the technology of bamboo fungus, is applied in the field of extraction technology of bamboo fungus colloidal polysaccharide, which can solve the problems of polysaccharide loss, separation of difficult polysaccharide solution and insoluble impurities, etc., so as to reduce workload, save centrifugal precipitation, The effect of improving efficiency

Active Publication Date: 2017-02-22
INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, no matter which part of Dictyophora is used to extract polysaccharides, it is only used to crush the raw materials, add water according to a certain ratio of material to liquid, and then heat and extract directly. After extraction, it needs to be centrifuged to obtain the upper polysaccharide solution; Due to its special physical and chemical structure, impurities and polysaccharides are easy to form jelly after extraction according to conventional methods, and it is extremely difficult to separate the polysaccharide solution from insoluble impurities, and the extraction process is likely to cause loss of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of extracting process of bamboo fungus fungus pectin polysaccharide, specifically comprises the following steps:

[0019] (1) Take the bamboo fungus fungus holder, wash it and remove the outer skin to obtain the middle bamboo fungus fungus holder gelatin, set aside;

[0020] (2) Crack the Dictyophora jelly obtained in step (1), homogenize it into a homogenate, and adjust the pH of the homogenate to 4;

[0021] (3) Freeze the Dictyophora jelly with the pH adjusted in step (2) at -20°C for 1 hour, then dissolve it completely at 20°C, and repeat the freezing and thawing process 10 times;

[0022] (4) Extract the Dictyophora japonicum after repeated freezing and thawing in step (3) at 60°C for 5 hours, filter, add 4 times the weight of 95wt% ethanol after the filtrate is concentrated, let it stand, and centrifuge to obtain a precipitate. After removing the protein Preparation of Dictyophora mycoplasma polysaccharide.

Embodiment 2

[0024] A kind of extracting process of bamboo fungus fungus pectin polysaccharide, specifically comprises the following steps:

[0025] (1) Take the bamboo fungus fungus holder, wash it and remove the outer skin to obtain the middle bamboo fungus fungus holder gelatin, set aside;

[0026] (2) Crack the Dictyophora jelly obtained in step (1), homogenize it into a homogenate, and adjust the pH of the homogenate to 10;

[0027] (3) Freeze the Dictyophora jelly with the pH adjusted in step (2) at 0°C for 24 hours, then dissolve it completely at 80°C, and repeat the freezing and thawing process once;

[0028] (4) Extract the Dictyophora jelly after repeated freezing and thawing in step (3) at 90°C for 1 hour, filter, add 6 times the weight of 95wt% ethanol after the filtrate is concentrated, let it stand, and centrifuge to obtain a precipitate. After removing the protein Preparation of Dictyophora mycoplasma polysaccharide.

Embodiment 3

[0030] A kind of extracting process of bamboo fungus fungus pectin polysaccharide, specifically comprises the following steps:

[0031] (1) Take the bamboo fungus fungus holder, wash it and remove the outer skin to obtain the middle bamboo fungus fungus holder gelatin, set aside;

[0032] (2) Break up the Dictyophora jelly obtained in step (1), homogenize into a homogenate, and adjust the pH of the homogenate to 7;

[0033] (3) Freeze the Dictyophora jelly with the pH adjusted in step (2) at -10°C for 12 hours, then dissolve it completely at 50°C, and repeat the freezing and thawing process 5 times;

[0034] (4) Extract the Dictyophora japonicum after repeated freezing and thawing in step (3) at 75°C for 2.5 hours, filter, add 5 times the weight of 95wt% ethanol after the filtrate is concentrated, let it stand, and centrifuge to obtain a precipitate and remove the protein Finally, Dictyophora japonicus pectin polysaccharide was obtained.

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PUM

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Abstract

The invention discloses an extracting technology of dictyophora indusiata volva colloid polysaccharide, and belongs to the technical field of medical biology extraction. A freeze-thaw method is adopted for extracting colloid polysaccharide, dictyophora indusiata volva is taken to remove cortex to obtain intermediate dictyophora indusiata volva colloid, homogenizing is performed, the pH is adjusted, then, freezing is performed for 1-24 h under the temperature from subzero 20 DEG C to 0 DEG C, then, thorough dissolution is performed under the temperature of 20-80 DEG C, freezing and thawing are performed one to ten times repeatedly, and finally extraction, concentration and filtering are performed to obtain the dictyophora indusiata volva colloid polysaccharide. The advantage that the dictyophora indusiata colloid is high in water content is sufficiently utilized, no water needs to be added, the workload of follow-up concentration is lowered, efficiency is greatly improved, the extracting technology belongs to pure physical methods, no other chemical components are added, and the technology is safe and convenient to implement.

Description

technical field [0001] The invention belongs to the technical field of medical bio-extraction, and in particular relates to an extraction process of Dictyophora japonicus jelly polysaccharide. Background technique [0002] In the bamboo fungus receptacle, there is a layer of glue between the outer skin and the inner membrane of the receptacle, which is called the receptacle jelly. Dictyophora jelly accounts for 10% of the fresh weight of the whole Dictyophora fruiting body, and Dictyophora fungus (edible part) accounts for 30%-40%, that is, for every 3-4 catties of fresh Dictyophora produced, there is 1 catties colloid. Dictyophora jelly is in the middle of the bamboo fungus tray, without contact with the outside world, safe and pollution-free, easy to obtain, just collect the fungus tray and remove the outer skin. Gum is rich in active substances such as polysaccharides, and studies have shown that the extract of Dictyophora gum has antioxidant activity. [0003] At prese...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 林陈强陈济琛张慧林戎斌郑力林新坚
Owner INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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